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pUC18_delLcho3510

A vector for gene replacement of Lcho_3510 with kanamycin-registance gene (Kmr).

Catalog number RDB19817
Resource name pUC18_delLcho3510
Clone info. A vector for gene replacement of Lcho_3510 with kanamycin-registance gene (Kmr).
Vector backbone pUC18-mob (plasmid)
Selectable markers Kanamycin
Gene/insert name Leptothrix cholodnii (SP-6 strain) Lcho_3510 genomic DNA; Bacillus subtilis spoVG genomic DNA; E. coli kanamycin resistance gene genomic DNA.
Depositor|Developer Kunoh, Tatsuki |
Other clones in our bank
External Database
Pseudomonas aeruginosa mob
    Leptothrix cholodnii (SP-6 strain) Lcho_3510
      Bacillus subtilis spoVG
        E. coli kanamycin resistance gene
            Reference sequence
              
             

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to DEPOSITOR and a citation of the literature designated by the DEPOSITOR are requested. (Kunoh, T. et al. Appl. Environ. Microbiol. e0134122, 2022. doi: 10.1128/aem.01341-22.)
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            提供条件 利用者は、研究成果の公表にあたって寄託者への謝辞の表明を必要とし、寄託者の指定する文献を引用すること(Kunoh, T. et al. Appl. Environ. Microbiol. e0134122, 2022. doi: 10.1128/aem.01341-22.)。
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            RDB19817 pUC18_delLcho3510 Under QC test. Please contact us. DNA solution

            check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

            How to cite this biological resource

            Materials & Methods section:

            The pUC18_delLcho3510 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19817).

            Reference section:

            Kunoh, T., Yamamoto, T., Prasad, M., Ono, E., Li, X., Sugimoto, S., Iida, E., Obana, N., Takeda, M., Nomura, N., Utada, A.S., Porous Pellicle Formation of a Filamentous Bacterium, Leptothrix. Appl. Environ. Microbiol. 88 (23): e0134122 (2022). PMID 36416549. [link to RRC of NBRP]

            Further references such as user reports and related articles (go to bottom)

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            QC test results

            check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

            References

            Original, user report and related articles

            original Kunoh, T., Porous Pellicle Formation of a Filamentous Bacterium, Leptothrix. Appl. Environ. Microbiol. 88 (23): e0134122 (2022). PMID 36416549. [link to RRC of NBRP]
            protocol Kunoh, T., Development of a Gene Replacement Method for the Filamentous Bacterium Leptothrix cholodnii SP-6. Bio Protoc. (8): e4652 (2023). PMID 37113333.

            2024.04.04

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