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Yellow Cameleon-Nano15/pcDNA3

Vector of Yellow Cameleon-Nano15, an ultrasensitive Ca2+ indicator with Kd = 15 nM, for mammalian expression.

Catalog number RDB19880
Resource name Yellow Cameleon-Nano15/pcDNA3
Clone info. Vector of Yellow Cameleon-Nano15, an ultrasensitive Ca2+ indicator with Kd = 15 nM, for mammalian expression. For more information, see Fig.1 of Horikawa, K. et al., Nat. Methods, 7 (9): 729-732, 2010.
Vector backbone pcDNA3 (plasmid)
Size of vector backbone 5.4 kb
Selectable markers Ampicillin (E. coli), Neomycin (mammalian cell)
Gene/insert name Aequorea victoria GFP (CFP), GFP (Venus); Xenopus laevis calmodulin; synthetic myosin light chain kinase peptide (M13).
Depositor|Developer Nagai, Takeharu |
Other clones in our bank

External Database
Aequorea victoria GFP (CFP)
    Aequorea victoria GFP (Venus)
      Xenopus laevis calmodulin Synthetic DNA synthetic myosin light chain kinase peptide (M13)

        Reference sequence
          
         
        Sequence (full) RDB19880hts01.seqcheck assembled from experimentally sequenced data
        Test sheet RDB19880_B3Acp1-1.pdf 

        Distribution information

        Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
        Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Horikawa, K. et al., Nat. Methods 7 (9): 729-732, 2010) The RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University.
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        提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Horikawa, K. et al., Nat. Methods 7 (9): 729-732, 2010)。利用者が非営利目的の教育・研究以外の目的に用いる場合は、大阪大学と別途MTAを締結すること。

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        Catalog # Resource name Shipping form Fee (non-profit org.)
        RDB19880 Yellow Cameleon-Nano15/pcDNA3 DNA solution


        How to cite this biological resource

        Materials & Methods section:

        The Yellow Cameleon-Nano15/pcDNA3 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19880).

        Reference section:

        Horikawa, K., Yamada, Y., Matsuda, T., Kobayashi, K., Hashimoto, M., Matsu-ura, T., Miyawaki, A., Michikawa, T., Mikoshiba, K., Nagai, T., Spontaneous network activity visualized by ultrasensitive Ca(2+) indicators, yellow Cameleon-Nano. Nat. Methods. 7 (9): 729-732 (2010). PMID 20693999. [link to RRC of NBRP]

        Further references such as user reports and related articles (go to bottom)


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        References

        Original, user report and related articles

        original Horikawa, K., Spontaneous network activity visualized by ultrasensitive Ca(2+) indicators, yellow Cameleon-Nano. Nat. Methods. 7 (9): 729-732 (2010). PMID 20693999. [link to RRC of NBRP]

        2023.10.05

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