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pMAMtracker-Green

A reporter plasmid based on split-GFP for evaluating intracellular organelle (mitochondria-associated membrane).

Catalog number RDB19896
Resource name pMAMtracker-Green
Clone info. A reporter plasmid based on split-GFP for evaluating intracellular organelle (mitochondria-associated membrane). See Fig.1 of Sakai, S. et al., FASEB J. 35 (7): e21688, 2021.
Comment Expression was confirmed by the depositor with western blotting, immunocytochemistry, live cell imaging.
Vector backbone pEGFP-C1 (plasmid)
Selectable markers Kanamycin (E. coli), Neomycin (mammalian cell)
Gene/insert name mouse Akap1 cDNA (mito signal), Discosoma sp. RFP (ddGFP), S. cerevisiae UBC6 (ER anchor).
Depositor|Developer Yamanaka, Koji |
Other clones in our bank
External Database
mouse Akap1 S. cerevisiae UBC6
        Reference sequence
          
         
        Sequence (full) RDB19896hts01.seqcheck assembled from experimentally sequenced data
        Test sheet RDB19896_B3D4p1-1.pdf 

        Distribution information

        Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
        Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (Sakai S. et al., FASEB J. 35(7):e21688, 2021, doi: 10.1096/fj.202100137R); (Alford SC. et al., ACS Synth Biol. 1(12):569-575, 2012, doi: 10.1021/sb300050j) Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT shall obtain approval from the DEPOSITOR using the Approval Form. The availability of the BIOLOGICAL RESOURCE is restricted to a RECIPIENT of a not-for profit organization for a not-for-profit research. The Recipient acknowledges and agrees the Limited Use Label License of Promega Corporation for the use of SmBiT vector DNA incorporated in the BIOLOGICAL RESOURCE. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
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        Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
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        Remarks
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        提供条件 利用者は、研究成果の公表にあたって開発者の指定する文献を引用する(Sakai S. et al., FASEB J. 35(7):e21688, 2021, doi: 10.1096/fj.202100137R); Alford SC. et al., ACS Synth Biol. 1(12):569-575, 2012, doi: 10.1021/sb300050j)。利用者は提供承諾書を用いて、事前に寄託者の承諾を得る。利用者は、非営利機関の学術研究に限る。利用者はプロメガ社が示すLimited Use Label Licenseに従って本件リソースに含まれるSmBiT vector DNAを取り扱う。利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び事業活動を行う場合は、寄託者と別途協議を行う。
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        提供依頼書 [Word]
        提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
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        手続きの概要は、「提供申込みについて[link]」をご覧ください。
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        寄託者の書面による提供承諾が必要です。

        Catalog # Resource name Shipping form Fee (non-profit org.)
        RDB19896 pMAMtracker-Green DNA solution

        How to cite this biological resource

        Materials & Methods section:

        The pMAMtracker-Green was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19896).

        Reference section:

        Sakai, S., Watanabe, S., Komine, O., Sobue, A., Yamanaka, K., Novel reporters of mitochondria-associated membranes (MAM), MAMtrackers, demonstrate MAM disruption as a common pathological feature in amyotrophic lateral sclerosis. FASEB J. 35 (7): e21688 (2021). PMID 34143516. [link to RRC of NBRP]

        Further references such as user reports and related articles (go to bottom)

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        References

        Original, user report and related articles

        original Sakai, S., Novel reporters of mitochondria-associated membranes (MAM), MAMtrackers, demonstrate MAM disruption as a common pathological feature in amyotrophic lateral sclerosis. FASEB J. 35 (7): e21688 (2021). PMID 34143516. [link to RRC of NBRP]

        2023.07.06

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