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pCSIIbsr-PKG-hNo.7

Lentivirus vector of cGMP biosensor, protein kinase G (PKG) #7 with blasticidine resistance gene.

Catalog number RDB19906
Resource name pCSIIbsr-PKG-hNo.7
Clone info. Lentivirus vector of cGMP biosensor, protein kinase G (PKG) #7 with blasticidine resistance gene. PKG #7 is composed of cGMP-binding domain B from cGMP-dependent protein kinase IB, which binds specifically to intracellular cGMP, and of the ECFP and cp variant of Venus, cp173Venus, as the FRET donor and acceptor, respectively. See Fig.1 of Hirota, K. et al., Endocrinology, 163 (3): bqac005 (2022).
Vector backbone pCSIIbsr (plasmid)
Selectable markers Ampicillin (E. coli), BSD (mammalian cell). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Gene/insert name human PRKG1 cDNA; Aequorea victoria GFP (ECFP, cp173Venus).
Depositor|Developer Matsuda, Michiyuki | Hirota, Keisho |
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External Database
human PRKG1 Aequorea victoria GFP
    Aequorea victoria GFP
          Reference sequence
            
           
          Sequence (full) RDB19906hts01.seqcheck assembled from experimentally sequenced data
          Test sheet RDB19906_B3C6p1-1.pdf 

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested.((Hirota, K et al. Endocrinology. 163(3): bqac005, 2022) For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Hirota, K et al. Endocrinology. 163(3): bqac005, 2022)。営利機関の利用希望者は、事前に利用条件等につき寄託者と合意し、提供承諾を得ること。
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          備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

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          RDB19906 pCSIIbsr-PKG-hNo.7 DNA solution

          Ordering instruction of plasmids [in Japanese] [in English]

          How to cite this biological resource

          Materials & Methods section:

          The pCSIIbsr-PKG-hNo.7 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19906).

          Reference section:

          Hirota, K., Hirashima, T., Horikawa, K., Yasoda, A., Matsuda, M., C-type Natriuretic Peptide-induced PKA Activation Promotes Endochondral Bone Formation in Hypertrophic Chondrocytes. Endocrinology 163 (3): bqac005 (2022). PMID 35041746. [link to RRC of NBRP]

          Further references such as user reports and related articles (go to bottom)

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          References

          Original, user report and related articles

          original Hirota, K., C-type Natriuretic Peptide-induced PKA Activation Promotes Endochondral Bone Formation in Hypertrophic Chondrocytes. Endocrinology 163 (3): bqac005 (2022). PMID 35041746. [link to RRC of NBRP]

          2023.11.23

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