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pCMV-myc-RNAi refractory shootin1a (S101D, S249D)

Expression vector to express RNAi refractory constitutively active (phosphomimic) human shootin1a tagged with myc at N-terminus.

Catalog number RDB19951
Resource name pCMV-myc-RNAi refractory shootin1a (S101D, S249D)
Clone info. Expression vector to express RNAi refractory constitutively active (phosphomimic) human shootin1a tagged with myc at N-terminus. The Pak1 phosphorylation sites Ser101 and Ser249 are replaced with aspartate. See Figs. 3 and 4 of Toriyama, M. et al., Curr. Biol., 23(6): 529-534, 2013.
Vector backbone pCMV-myc (pCMV-tag 3B) (plasmid)
Selectable markers Kanamycin (E. coli), Neomycin (mammalian cell)
Gene/insert name human SHTN1 cDNA; synthetic Myc-tag.
Depositor|Developer Inagaki, Naoyuki |
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human SHTN1
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          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Toriyama M., et al. Curr Biol. 23(6):529-34, 2013) and an acknowledgment to the DEPOSITOR are requested. For use of the BIOLOGICAL RESOURCE by a for-profit organization　and by a not-for profit organization for a for-profit research, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献(Toriyama M., et al. Curr Biol. 23(6):529-34, 2013)を引用し、謝辞の表明を必要とする。営利機関並びに学術機関の営利目的利用の希望者は、事前に利用条件等につき寄託者と合意し、提供承諾を得ること。
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          RDB19951 pCMV-myc-RNAi refractory shootin1a (S101D, S249D) Under QC test. Please contact us. DNA solution

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          How to cite this biological resource

          Materials & Methods section:

          The pCMV-myc-RNAi refractory shootin1a (S101D, S249D) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19951).

          Reference section:

          Toriyama, M., Kozawa, S., Sakumura, Y., Inagaki, N., Conversion of a signal into forces for axon outgrowth through Pak1-mediated shootin1 phosphorylation. Curr. Biol. 23 (6): 529-534 (2013). PMID 23453953. [link to RRC of NBRP]

          Further references such as user reports and related articles (go to bottom)

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          QC test results

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          References

          Original, user report and related articles

          original Toriyama, M., Conversion of a signal into forces for axon outgrowth through Pak1-mediated shootin1 phosphorylation. Curr. Biol. 23 (6): 529-534 (2013). PMID 23453953. [link to RRC of NBRP]

          2023.12.11

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