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pMRX-No-HaloTag7-mGFP-LC3-mRFP

Retroviral vector for stable expression of HaloTag7-mGFP-LC3-mRFP to measure autophagic flux.

Catalog number RDB19965
Resource name pMRX-No-HaloTag7-mGFP-LC3-mRFP
Clone info. Retroviral vector for stable expression of HaloTag7-mGFP-rat LC3-mRFP without a drug resistance marker to measure autophagic flux. See, Yim, W.WY. et al., eLife 11: e78923, 2022.
Comment Expression was confirmed by the depositor with western blotting.
Vector backbone pMRX-No (plasmid)
Selectable markers Ampicillin (E. coli)
Gene/insert name Rhodococcus rhodochrous HaloTag7; Aequorea victoria GFP (mGFP); rat LC3 cDNA; Discosoma sp. RFP (mRFP).
Depositor|Developer Mizushima, Noboru |
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External Database
rat Map1lc3b
          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.(Willa Wen-You Yim et al. eLife. 11:e78923, 2022)
          Additional terms and conditions:
          Regarding pMRX vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literatures for pMX and pMRX vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003; Saitoh T., J. Biol. Chem. 278 (38): 36005-36012, 2003) is requested.
          The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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          Remarks Remember that you will be working with samples containing infectious virus.
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用すること(Willa Wen-You Yim et al. eLife. 11:e78923, 2022)。
          付加的提供条件：
          pMRXベクターについて：利用者は、研究成果の公表にあたって pMXならびにpMRXベクターの文献 （Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003; Saitoh T., J. Biol. Chem. 278 (38): 36005-36012, 2003) の引用を必要とする。
          本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。
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          備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

          Catalog # Resource name Availability Shipping form Fee (non-profit org.)
          RDB19965 pMRX-No-HaloTag7-mGFP-LC3-mRFP Under QC test. Please contact us. DNA solution

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          How to cite this biological resource

          Materials & Methods section:

          The pMRX-No-HaloTag7-mGFP-LC3-mRFP was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19965).

          Reference section:

          Yim, W.W., Yamamoto, H., Mizushima, N., A pulse-chasable reporter processing assay for mammalian autophagic flux with HaloTag. Elife 11: e78923 (2022). PMID 35938926. [link to RRC of NBRP]

          Further references such as user reports and related articles (go to bottom)

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          QC test results

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          References

          Original, user report and related articles

          original Yim, W.W., A pulse-chasable reporter processing assay for mammalian autophagic flux with HaloTag. Elife 11: e78923 (2022). PMID 35938926. [link to RRC of NBRP]

          2023.12.13

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