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pMRX-No-HaloTag7-mGFP-LC3

Retroviral vector for stable expression of HaloTag7-mGFP-LC3 to measure autophagic flux.

Catalog number RDB19966
Resource name pMRX-No-HaloTag7-mGFP-LC3
Clone info. Retroviral vector for stable expression of HaloTag7-mGFP-rat LC3 without a drug resistance marker to measure autophagic flux. See, Yim, W.WY. et al., eLife 11: e78923, 2022.
Comment Expression was confirmed by the depositor with western blotting.
Vector backbone pMRX-No (plasmid)
Selectable markers Ampicillin (E. coli)
Gene/insert name Rhodococcus rhodochrous HaloTag7; Aequorea victoria GFP (mGFP); rat LC3 cDNA.
Depositor|Developer Mizushima, Noboru |
Other clones in our bank
External Database
rat Map1lc3b
          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.(Willa Wen-You Yim et al. eLife. 11:e78923, 2022)
          Additional terms and conditions:
          Regarding pMRX vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literatures for pMX and pMRX vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003; Saitoh T., J. Biol. Chem. 278 (38): 36005-36012, 2003) is requested.
          The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
          Ordering Please visit Information of Request for Distribution.[link] 
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          Remarks Remember that you will be working with samples containing infectious virus.
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用すること(Willa Wen-You Yim et al. eLife. 11:e78923, 2022)。
          付加的提供条件：
          pMRXベクターについて：利用者は、研究成果の公表にあたって pMXならびにpMRXベクターの文献 （Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003; Saitoh T., J. Biol. Chem. 278 (38): 36005-36012, 2003) の引用を必要とする。
          本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

          Catalog # Resource name Availability Shipping form Fee (non-profit org.)
          RDB19966 pMRX-No-HaloTag7-mGFP-LC3 Under QC test. Please contact us. DNA solution

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          How to cite this biological resource

          Materials & Methods section:

          The pMRX-No-HaloTag7-mGFP-LC3 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19966).

          Reference section:

          Yim, W.W., Yamamoto, H., Mizushima, N., A pulse-chasable reporter processing assay for mammalian autophagic flux with HaloTag. Elife 11: e78923 (2022). PMID 35938926. [link to RRC of NBRP]

          Further references such as user reports and related articles (go to bottom)

          References and tips

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          QC test results

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          References

          Original, user report and related articles

          original Yim, W.W., A pulse-chasable reporter processing assay for mammalian autophagic flux with HaloTag. Elife 11: e78923 (2022). PMID 35938926. [link to RRC of NBRP]

          2023.06.05

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