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MFCS1-pshh-luciferace

Luciferace reporter vector under the control of Xenopus MFCS1 enhancer and shh promoter.

Catalog number RDB19990
Resource name MFCS1-pshh-luciferace
Alternative name MFCS1-shh promoter-luc2
Clone info. Luciferace reporter vector under the control of Xenopus MFCS1 enhancer and shh promoter.
Comment Expression was confirmed by the depositor by luciferase assay
Vector backbone pGL4.23 (plasmid)
Selectable markers Ampicillin (E. coli)
Gene/insert name Xenopus laevis Zrs (MFCS1) genomic DNA; Xenopus tropicalis shh enomic DNA.
Depositor|Developer Amano, Takanori |
Other clones in our bank
External Database
Xenopus laevis Zrs (MFCS1)
    Xenopus tropicalis shh
          Reference sequence
            
           
          Sequence (full) RDB19990hts01.seqcheck assembled from experimentally sequenced data
          Test sheet RDB19990_B3G7p1-1.pdf 

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a citation of literature(s) designated by the DEPOSITOR are requested. (Tada R et al., Dev Biol. S0012-1606(23)00093-3. doi: 10.1016/j.ydbio.2023.05.009. 2023)
          Additional terms and conditions:
          The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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          提供条件 利用者は、研究成果の公表にあたって謝辞の表明を必要とし、寄託者の指定する文献を引用すること(Tada R et al., Dev Biol. S0012-1606(23)00093-3. doi: 10.1016/j.ydbio.2023.05.009. 2023)。
          付加的提供条件：
          本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。
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          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB19990 MFCS1-pshh-luciferace DNA solution

          How to cite this biological resource

          Materials & Methods section:

          The MFCS1-pshh-luciferace was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19990).

          Reference section:

          Tada, R., Higashidate, T., Amano, T., Ishikawa, S., Yokoyama, C., Kobari, S., Nara, S., Ishida, K., Kawaguchi, A., Ochi, H., Ogino, H., Yakushiji-Kaminatsui, N., Sakamoto, J., Kamei, Y., Tamura, K., Yokoyama, H., The shh limb enhancer is activated in patterned limb regeneration but not in hypomorphic limb regeneration in Xenopus laevis. Dev. Biol. 500: 22-30 (2023). PMID 37247832. [link to RRC of NBRP]

          Further references such as user reports and related articles (go to bottom)

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          References

          Original, user report and related articles

          original Tada, R., The shh limb enhancer is activated in patterned limb regeneration but not in hypomorphic limb regeneration in Xenopus laevis. Dev. Biol. 500: 22-30 (2023). PMID 37247832. [link to RRC of NBRP]

          2024.04.04

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