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CSII-CMV-cofilin

Lentivirus expression vector of human cofilin.

Catalog number RDB20019
Resource name CSII-CMV-cofilin
Clone info. Lentivirus expression vector of human cofilin. See Fig.4 of Nakajima, M. et al., FEBS Lett. 597 (8): 1114-1124 (2023).
Comment Full-length human CFL cDNA was amplified from an HT1080 cell cDNA library.
Vector backbone CSII-CMV-MCS (plasmid)
Selectable markers Ampicillin (E. coli)
Growth remarks Please note that Zeo resistance marker is not included in the lentivirus produced from this vector. E. coli: Amp^r. NEB Stable or an equivalent host is recommended for amplifying this clone.
Gene/insert name human CFL1 cDNA.
Depositor|Developer Simizu, Siro |
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human CFL1
          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.(Nakajima, M. et al. FEBS Lett. 597(8): 1114-1124, 2023)
          Additional terms and conditions:
          Regarding lentivirus vector: The RECIPIENT agrees to expressly describe Dr. Hiroyuki Miyoshi as the Developer of the lentivirus vector. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose.
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          Remarks Remember that you will be working with samples containing infectious virus.
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用すること(Nakajima, M. et al. FEBS Lett. 597(8): 1114-1124, 2023)。
          付加的提供条件：
          レンチウイルスベクターについて：本件研究材料を利用した研究結果等を発表する際は、レンチウイルスベクターが三好浩之博士により開発されたことを明示する。本件リソースの使用は学術機関での学術研究に限る。
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          遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
          手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
          備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

          Catalog # Resource name Availability Shipping form Fee (non-profit org.)
          RDB20019 CSII-CMV-cofilin Under QC test. Please contact us. DNA solution

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.
          Ordering instruction of plasmids [in Japanese] [in English]

          How to cite this biological resource

          Materials & Methods section:

          The CSII-CMV-cofilin was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB20019).

          Reference section:

          Nakajima, M., Kawahara, R., Simizu, S., Cofilin promotes vasculogenic mimicry by regulating the actin cytoskeleton in human breast cancer cells. FEBS Lett. 597 (8): 1114-1124 (2023). PMID 36737242. [link to RRC of NBRP]

          Further references such as user reports and related articles (go to bottom)

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          Featured content Lentiviral plasmid list (English text)

          QC test results

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          References

          Original, user report and related articles

          original Nakajima, M., Cofilin promotes vasculogenic mimicry by regulating the actin cytoskeleton in human breast cancer cells. FEBS Lett. 597 (8): 1114-1124 (2023). PMID 36737242. [link to RRC of NBRP]

          2024.04.04

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