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EGFP-VSVG-KDELR2 (#RDB20107)

Expression vector for mammalian cells. Preproglucagon signal sequence, EGFP, luminal region of VSVG (tsO45), TM and cytoplasmic region of human KDELR2.


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Saitoh, A., J. Biol. Chem. 284 (20): 13948-13957 (2009). PMID 19299515. [PubMed] [Article] [RRC of NBRP]
Test sheet Data Sheet open 
 
Clone info. Expression vector for mammalian cells. Preproglucagon signal sequence, EGFP, luminal region of VSVG (tsO45), TM and cytoplasmic region of human KDELR2.
Vector backbone pCI-neo (plasmid)
Selectable markers Ampicillin (E. coli), Neomycin (mammalian cells).
Growth conditions LB+Ampicilin, 37oC
Gene/insert name human KDELR2 cDNA; Aequorea victoria GFP (EGFP) cDNA; Vesicular stomatitis G-protein luminal region-tag; golden hamster preproglucagon signal sequence cDNA.
Depositor|Developer Nakayama, Kazuhisa |

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Saitoh A., et al. J. Biol. Chem. 284(20):13948-13957, 2009) The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit organization for a not-for-profit research.　
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Saitoh A., et al. J. Biol. Chem. 284(20):13948-13957, 2009)。非営利学術機関の学術研究に限る。

Catalog # Resource name Shipping form Fee
RDB20107 EGFP-VSVG-KDELR2 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

How to cite this biological resource

Materials & Methods section:

The EGFP-VSVG-KDELR2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB20107).

Reference section:

Saitoh, A., Shin, H.W., Yamada, A., Waguri, S., Nakayama, K., Three homologous ArfGAPs participate in coat protein I-mediated transport. J. Biol. Chem. 284 (20): 13948-13957 (2009). PMID 19299515. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Saitoh, A., Three homologous ArfGAPs participate in coat protein I-mediated transport. J. Biol. Chem. 284 (20): 13948-13957 (2009). PMID 19299515. [PubMed] [Article] [RRC of NBRP]