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pcDNA3-HA-GBF1 (#RDB20111)

Expression vector of human GBF1 for mammalian cells, N-teminal HA-tag.


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Kawamoto, K., Traffic 3 (7): 483-495 (2002). PMID 12047556. [PubMed] [Article] [RRC of NBRP]
Test sheet Data Sheet open 
 
Clone info. Expression vector of human GBF1 for mammalian cells, N-teminal HA-tag.
Vector backbone pcDNA3 (plasmid)
Selectable markers Ampicillin (E. coli), Neomycin (mammalian cell)
Growth conditions LB+Ampicilin, 37℃
Gene/insert name human GBF1 cDNA.
Depositor|Developer Nakayama, Kazuhisa |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kawamoto A., et al. Traffic, 3(7):483-495, 2022) The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit organization for a not-for-profit research.　
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kawamoto A., et al. Traffic, 3(7):483-495, 2022)。非営利学術機関の学術研究に限る。

Catalog # Resource name Shipping form Fee
RDB20111 pcDNA3-HA-GBF1 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

How to cite this biological resource

Materials & Methods section:

The pcDNA3-HA-GBF1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB20111).

Reference section:

Kawamoto, K., Yoshida, Y., Tamaki, H., Torii, S., Shinotsuka, C., Yamashina, S., Nakayama, K., GBF1, a guanine nucleotide exchange factor for ADP-ribosylation factors, is localized to the cis-Golgi and involved in membrane association of the COPI coat. Traffic 3 (7): 483-495 (2002). PMID 12047556. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Kawamoto, K., GBF1, a guanine nucleotide exchange factor for ADP-ribosylation factors, is localized to the cis-Golgi and involved in membrane association of the COPI coat. Traffic 3 (7): 483-495 (2002). PMID 12047556. [PubMed] [Article] [RRC of NBRP]