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pCDF-Mm2

Expression vector of the variant of Methanosarcina mazei PylRS specific for pyrrolysine derivatives (Boc-Lys, Az-ZLys, AzAmZLys) and its cognate Pyl tRNA.

Catalog number RDB20116
Resource name pCDF-Mm2
Clone info. Expression vector of the variant of Methanosarcina mazei PylRS specific for pyrrolysine derivatives (Boc-Lys, Az-ZLys, AzAmZLys) and its cognate Pyl tRNA. See, Yamaguchi, A. et al., Bioconjug. Chem., 27 (1): 198-206, 2016.
Comment MutationLys61, Glu131, Ala306, Phe384, Glu444 (PylRS)
Vector backbone pCDF-1b (kanamycin-resistant derivative) (plasmid)
Selectable markers E. coli (Kanamycin)
Growth remarks Drug resistance has been replaced by the kanamycin resistance gene.
Gene/insert name Methanosarcina mazei PylRS, tRNA
Depositor|Developer Tagami, Shunsuke | Sakamoto, Kensaku |
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External Database
Methanosarcina mazei PylRS, tRNA
            Reference sequence
              
             
            Sequence (full) RDB20116hts01.seqcheck assembled from experimentally sequenced data
            Test sheet RDB20116_B3Jcp1-1.pdf 

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Atsushi Y. et al., Bioconjug Chem. 27(1): 198-206, 2015) and an acknowledgment to the DEPOSITOR are requested. The same Terms and Conditions apply to for-profit use as to not-for-profit use conducted by academic institutions.
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            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献(Atsushi Y. et al., Bioconjug Chem. 27(1): 198-206, 2015)を引用し、謝辞の表明を必要とする。営利目的利用についても、学術機関の学術利用目的利用と同じ提供条件を適用する。
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            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB20116 pCDF-Mm2 DNA solution

            How to cite this biological resource

            Materials & Methods section:

            The pCDF-Mm2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB20116).

            Reference section:

            Yamaguchi, A., Matsuda, T., Ohtake, K., Yanagisawa, T., Yokoyama, S., Fujiwara, Y., Watanabe, T., Hohsaka, T., Sakamoto, K., Incorporation of a Doubly Functionalized Synthetic Amino Acid into Proteins for Creating Chemical and Light-Induced Conjugates. Bioconjug. Chem. 27 (1): 198-206 (2016). PMID 26625213. [link to RRC of NBRP]

            Further references such as user reports and related articles (go to bottom)

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            References

            Original, user report and related articles

            original Yamaguchi, A., Incorporation of a Doubly Functionalized Synthetic Amino Acid into Proteins for Creating Chemical and Light-Induced Conjugates. Bioconjug. Chem. 27 (1): 198-206 (2016). PMID 26625213. [link to RRC of NBRP]

            2023.11.14

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