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pMx/ATP8B4-HA

Retroviral vector for expression of human ATP8B4 in mammalian cells, C-terminal HA-tag.

Catalog number RDB20151
Resource name pMx/ATP8B4-HA
Clone info. Retroviral vector for expression of human ATP8B4 in mammalian cells, C-terminal HA-tag. The plasmid requires co-transfection with RDB20145 pcDNA3/HF-CDC50A to ensure the cellular localization of the encoded protein upon overexpression. (References: Takatsu et al., JBC 2011, 286, 38159; Bryde et al., JBC 2010, 285, 40562; van der Velden et al., JBC 2010, 285, 40088)
Vector backbone pMX (plasmid)
Gene/insert name human ATP8B4 cDNA; HA-tag.
Depositor|Developer Shin, Hye-Won |
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human ATP8B4
          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Takatsu, H. et al., J. Biol. Chem. 289(48): 33543-33556, 2014) The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for-profit organization for a not-for-profit research.
          Additional terms and conditions:
          Regarding pMXs vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs vector (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.
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          Remarks Remember that you will be working with samples containing infectious virus.
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Takatsu, H. et al., J. Biol. Chem. 289(48): 33543-33556, 2014)。非営利学術機関の学術研究に限る。
          付加的提供条件：
          pMXsベクターについて：利用者は、研究成果の公表にあたって pMXsベクターの文献 （Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) を引用し、東京大学医科学研究所　北村俊雄先生への謝辞の表明を必要とする。
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          提供同意書 (MTA, 非営利学術目的用)[Word]
          手続きの概要は、「提供申込みについて[link]」をご覧ください。
          備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

          Catalog # Resource name Availability Shipping form Fee (non-profit org.)
          RDB20151 pMx/ATP8B4-HA Under QC test. Please contact us. DNA solution

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          How to cite this biological resource

          Materials & Methods section:

          The pMx/ATP8B4-HA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB20151).

          Reference section:

          Takatsu, H., Tanaka, G., Segawa, K., Suzuki, J., Nagata, S., Nakayama, K., Shin, H.W., Phospholipid flippase activities and substrate specificities of human type IV P-type ATPases localized to the plasma membrane. J. Biol. Chem. 289 (48): 33543-33556 (2014). PMID 25315773. [link to RRC of NBRP]

          Further references such as user reports and related articles (go to bottom)

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          QC test results

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          References

          Original, user report and related articles

          original Takatsu, H., Phospholipid flippase activities and substrate specificities of human type IV P-type ATPases localized to the plasma membrane. J. Biol. Chem. 289 (48): 33543-33556 (2014). PMID 25315773. [link to RRC of NBRP]

          2024.04.23

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