Resource data sheet
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pcDNA3/mStayGold(c4)=UtrCH

Expression vector of mStayGold for filamentous actin labeling.

Catalog number RDB20221
Resource name pcDNA3/mStayGold(c4)=UtrCH
Clone info. Expression vector of mStayGold for filamentous actin labeling. Alternatively, the UtrCH gene can be replaced with a target gene for N-terminal tagging with mStayGold via a c4 adaptor and a linker [(GGGGS)3] (denoted as '=' in the resource name).
Vector backbone pcDNA3 (plasmid)
Gene/insert name Cytaeis uchidae GFP (mStayGold); human UtrCH (UTRN).
Depositor|Developer Miyawaki, Atsushi | Ando, Ryoko |
Other clones in our bank

External Database
Cytaeis uchidae GFP
    human UTRN

          Reference sequence
            
           
          Sequence (full) RDB20221hts01.seqcheck assembled from experimentally sequenced data
          Test sheet RDB20221_B3L4p1-1.pdf 

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution 1.The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
          2.A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
          3.In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
          Ando, R. et al., Nat. Methods. 2023 Nov 30. doi: 10.1038/s41592-023-02085-6.
          4.The Recipient’s Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
          5.For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with RIKEN Innovation Co., Ltd. and must obtain a prior written consent from the DEPOSITOR.
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          MTA, for use for not-for-profit academic purpose [Word]
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          Approval form [Word] by Dr. Atsushi Miyawaki
          Please visit Information of Request for Distribution.[link] 
          Remarks
          Please obtain written approval of Dr. Atsushi Miyawaki.
          提供案内 (日本国内) [open/close]

          提供条件 1.The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
          2.A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
          3.In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
          Ando, R. et al., Nat. Methods. 2023 Nov 30. doi: 10.1038/s41592-023-02085-6.
          4.The Recipient’s Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
          5.For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with RIKEN Innovation Co., Ltd. and must obtain a prior written consent from the DEPOSITOR.
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          手続きの概要は、「提供申込みについて[link]」をご覧ください。
          備考 提供条件は英文のまま記載してください。
          宮脇敦史博士の書面による提供承諾が必要です。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB20221 pcDNA3/mStayGold(c4)=UtrCH DNA solution


          How to cite this biological resource

          Materials & Methods section:

          The pcDNA3/mStayGold(c4)=UtrCH was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB20221).

          Reference section:

          Ando, R., Shimozono, S., Ago, H., Takagi, M., Sugiyama, M., Kurokawa, H., Hirano, M., Niino, Y., Ueno, G., Ishidate, F., Fujiwara, T., Okada, Y., Yamamoto, M., Miyawaki, A., StayGold variants for molecular fusion and membrane-targeting applications. Nat Methods 2023 Nov 30. doi: 10.1038/s41592-023-02085-6. Epub ahead of print PMID 38036853. [link to RRC of NBRP]

          Further references such as user reports and related articles (go to bottom)


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          References

          Original, user report and related articles

          original Ando, R., StayGold variants for molecular fusion and membrane-targeting applications. Nat Methods 2023 Nov 30. doi: 10.1038/s41592-023-02085-6. Epub ahead of print PMID 38036853. [link to RRC of NBRP]

          2024.04.04

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