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pBS Coupler4/td8ox2StayGold (c4)

Plasmid vector of td8ox2StayGold(c4) for fusion to the N-terminus of a target molecule (X) through a Coupler linker: (GGGGS)x3.

Catalog number RDB20226
Resource name pBS Coupler4/td8ox2StayGold (c4)
Clone info. For fusion of td8ox2StayGold(c4) to the N-terminus of a target molecule (X) through a Coupler linker: (GGGGS)x3. td8ox2StayGold is an intramolecular dimeric unit that performs as a monomer, and is composed of n1, oxStayGold(Y169F), PT, a 21-residue linker, n1, oxStayGold(Y169F), and c4. n1 is the appendage that facilitates fusions to the N-terminus of oxStayGold(Y169F). PT and c4 are the appendages that facilitate fusions to the C-terminus of oxStayGold(Y169F). td8ox2StayGold(c4) encoded by this plasmid DNA is followed by a Coupler linker: (GGGGS)×3. See, Supp. Fig.14 of Ando, R. et al. Nat. Methods, 2023. https://doi.org/10.1038/s41592-023-02085-6 for more information.
Vector backbone pBS Coulper4 (plasmid)
Gene/insert name Cytaeis uchidae GFP (StayGold) cDNA.
Depositor|Developer Miyawaki, Atsushi | Ando, Ryoko |
Other clones in our bank
External Database
Cytaeis uchidae GFP (StayGold)
            Reference sequence
              
             
            Sequence (full) RDB20226hts01.seqcheck assembled from experimentally sequenced data
            Test sheet RDB20226_B3Lsp1-1.pdf 

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution 1.The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
            2.A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
            3.In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
            Ando, R. et al., Nat. Methods. 2023 Nov 30. doi: 10.1038/s41592-023-02085-6.
            4.The Recipient’s Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
            5.For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with RIKEN Innovation Co., Ltd. and must obtain a prior written consent from the DEPOSITOR.
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            Approval form [Word] by Dr. Atsushi Miyawaki
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            Please obtain written approval of Dr. Atsushi Miyawaki.
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            提供条件 1.The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
            2.A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
            3.In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
            Ando, R. et al., Nat. Methods. 2023 Nov 30. doi: 10.1038/s41592-023-02085-6.
            4.The Recipient’s Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
            5.For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with RIKEN Innovation Co., Ltd. and must obtain a prior written consent from the DEPOSITOR.
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            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB20226 pBS Coupler4/td8ox2StayGold (c4) DNA solution

            How to cite this biological resource

            Materials & Methods section:

            The pBS Coupler4/td8ox2StayGold (c4) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB20226).

            Reference section:

            Ando, R., Shimozono, S., Ago, H., Takagi, M., Sugiyama, M., Kurokawa, H., Hirano, M., Niino, Y., Ueno, G., Ishidate, F., Fujiwara, T., Okada, Y., Yamamoto, M., Miyawaki, A., StayGold variants for molecular fusion and membrane-targeting applications. Nat Methods 2023 Nov 30. doi: 10.1038/s41592-023-02085-6. Epub ahead of print PMID 38036853. [link to RRC of NBRP]

            Further references such as user reports and related articles (go to bottom)

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            References

            Original, user report and related articles

            original Ando, R., StayGold variants for molecular fusion and membrane-targeting applications. Nat Methods 2023 Nov 30. doi: 10.1038/s41592-023-02085-6. Epub ahead of print PMID 38036853. [link to RRC of NBRP]

            2024.04.04

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