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pcDNA3/Cox8a=mStayGold

Expression vector of mStayGold as a fluorescent marker of the inner mitochondrial membrane.

Catalog number RDB20228
Resource name pcDNA3/Cox8a=mStayGold
Clone info. A fluorescent marker of the inner mitochondrial membrane. Cytochrome c oxidase subunit 8A (COX8A) was fused to the N-terminus of mStayGold through a Coupler linker: (GGGGS)×3, denoted as ‘=’. See Ando, R. et al. Nat. Methods, 2023. https://doi.org/10.1038/s41592-023-02085-6 for more information.
Vector backbone pcDNA3 (plasmid)
Gene/insert name Cytaeis uchidae GFP (StayGold) cDNA; mouse Cox8a cDNA.
Depositor|Developer Miyawaki, Atsushi | Ando, Ryoko |
Other clones in our bank
External Database
Cytaeis uchidae GFP (StayGold)
    mouse Cox8a
          Reference sequence
            
           
          Sequence (full) RDB20228hts01.seqcheck assembled from experimentally sequenced data
          Test sheet RDB20228_B3Lsp1-1.pdf 

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution 1.The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
          2.A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
          3.In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
          Ando, R. et al., Nat. Methods. 2023 Nov 30. doi: 10.1038/s41592-023-02085-6.
          4.The Recipient’s Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
          5.For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with RIKEN Innovation Co., Ltd. and must obtain a prior written consent from the DEPOSITOR.
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          Approval form [Word] by Dr. Atsushi Miyawaki
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          Remarks
          Please obtain written approval of Dr. Atsushi Miyawaki.
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          提供条件 1.The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
          2.A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
          3.In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
          Ando, R. et al., Nat. Methods. 2023 Nov 30. doi: 10.1038/s41592-023-02085-6.
          4.The Recipient’s Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
          5.For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with RIKEN Innovation Co., Ltd. and must obtain a prior written consent from the DEPOSITOR.
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          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB20228 pcDNA3/Cox8a=mStayGold DNA solution

          How to cite this biological resource

          Materials & Methods section:

          The pcDNA3/Cox8a=mStayGold was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB20228).

          Reference section:

          Ando, R., Shimozono, S., Ago, H., Takagi, M., Sugiyama, M., Kurokawa, H., Hirano, M., Niino, Y., Ueno, G., Ishidate, F., Fujiwara, T., Okada, Y., Yamamoto, M., Miyawaki, A., StayGold variants for molecular fusion and membrane-targeting applications. Nat Methods 2023 Nov 30. doi: 10.1038/s41592-023-02085-6. Epub ahead of print PMID 38036853. [link to RRC of NBRP]

          Further references such as user reports and related articles (go to bottom)

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          References

          Original, user report and related articles

          original Ando, R., StayGold variants for molecular fusion and membrane-targeting applications. Nat Methods 2023 Nov 30. doi: 10.1038/s41592-023-02085-6. Epub ahead of print PMID 38036853. [link to RRC of NBRP]

          2024.04.04

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