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pEB6CAGMCS SVBsd (#RDB20296)

Gene expression vector useful for rapid semi-stable transfectio in primate cells. Keyword:mini-oriP, episomal vector.


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Tanaka, J., Biochem. Biophys. Res. Commun. 264 (3): 938-943 (1999). PMID 10544034. [PubMed] [Article] [RRC of NBRP]
Test sheet Data Sheet open 
 
Clone info. Gene expression vector useful for rapid semi-stable transfection in primate cells. Keyword:mini-oriP, episomal vector.
Vector backbone pEB6CAGMCS Kan/Neo (RDB20293) (plasmid)
Growth conditions LB+Bsd(plate_100µg/mL, liquid_50µg/mL), 37oC
Growth remarks XL-10 is recommended as host. Culturing with DH5a may cause mutations.
Depositor|Developer Kijima, Junko |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Tanaka, J. et al., Biochem. Biophys. Res. Commun. 64(3): 938-943, 1999) and an acknowledgment to the DEPOSITOR are requested.
Additional terms and conditions:
Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献(Tanaka, J. et al., Biochem. Biophys. Res. Commun. 64(3): 938-943, 1999)を引用し、謝辞の表明を必要とする。
付加的使用条件：
CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。

Catalog # Resource name Shipping form Fee
RDB20296 pEB6CAGMCS SVBsd DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

How to cite this biological resource

Materials & Methods section:

The pEB6CAGMCS SVBsd was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB20296).

Reference section:

Tanaka, J., Miwa, Y., Miyoshi, K., Ueno, A., Inoue, H., Construction of Epstein-Barr virus-based expression vector containing mini-oriP. Biochem. Biophys. Res. Commun. 264 (3): 938-943 (1999). PMID 10544034. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Tanaka, J., Construction of Epstein-Barr virus-based expression vector containing mini-oriP. Biochem. Biophys. Res. Commun. 264 (3): 938-943 (1999). PMID 10544034. [PubMed] [Article] [RRC of NBRP]