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gRNA_B (#RDB20779)

Expression vector of sgRNA of mouse Sox9 distal enhancer, mXYSRa.


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Ogawa, Y., Commun. Biol. 8 (1): 81 (2025). PMID 39827212. [PubMed] [Article] [RRC of NBRP]
Test sheet Data Sheet open 
 
Clone info. Expression vector of sgRNA of mouse Sox9 distal enhancer, mXYSRa.
Vector backbone pCR-Blunt II-TOPO (plasmid)
Selectable markers Kanamycin, Zeocin (E. coli)
Gene/insert name Synthetic mouse Sox9 oligo DNA; human U6 promoter; gRNA scafold.
Depositor|Developer Takada, Shuji |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (Ogawa, Y. et al., Commun. Biol. 8(1):81 , 2025 doi:10.1038/s42003-025-07504-2) For use of the BIOLOGICAL RESOURCE by a for-profit organization or for use of the BIOLOGICAL RESOURCE for for-profit research by not-for-profit organization, the RECIPIENT must obtain a prior written consent from the National Research Institute for Child Health and Development.
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必要書類
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提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
提供同意書 (MTA, 営利目的用)[Word]
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Ogawa, Y. et al., Commun. Biol. 8(1):81 , 2025 doi:10.1038/s42003-025-07504-2)。営利機関の利用もしくは非営利学術機関による営利目的研究利用の場合は、国立成育医療研究センターの許可を得ること。　

Catalog # Resource name Shipping form Fee
RDB20779 gRNA_B DNA solution JPY 9,460 (not-for-profit academic purpose)
JPY 18,920 (for-profit-research purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

How to cite this biological resource

Materials & Methods section:

The gRNA_B was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB20779).

Reference section:

Ogawa, Y., Tsuchiya, I., Yanai, S., Baba, T., Morohashi, K.I., Sasaki, T., Sasaki, J., Terao, M., Tsuji-Hosokawa, A., Takada, S., GATA4 binding to the Sox9 enhancer mXYSRa/Enh13 is critical for testis differentiation in mouse. Commun. Biol. 8 (1): 81 (2025). PMID 39827212. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Ogawa, Y., GATA4 binding to the Sox9 enhancer mXYSRa/Enh13 is critical for testis differentiation in mouse. Commun. Biol. 8 (1): 81 (2025). PMID 39827212. [PubMed] [Article] [RRC of NBRP]