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23/C11 - S. pombe Postgenome Database Clone

 Plasmid request
Clone ID at the S. pombe Postgenome Database 23/C11
NCBI Gene ID 2539179
Gene Symbol sds21
Protein Name serine/threonine protein phosphatase Sds21
Synonym SPCC31H12.05c
RefSeq Protein NP_587898.1
PomBase(1) SPCC31H12.05c
KEGG(2) spo:SPCC31H12.05c
Depositor-supplied data(3) 23C11
Note  

(1) The PomBase, funded by the Wellcome Trust and is run by a consortium comprising the University of Cambridge, the European Bioinformatics Institute and University College London.

(2) The Kyoto Encyclopedia of Genes and Genomes of the Kanehisa Laboratories in the Bioinformatics Center of Kyoto University and the Human Genome Center of the University of Tokyo.

(3) Primer sequences for constructing an entry clone and known sequence variation are available at this site, the S. pombe Postgenome Database of the Chemical Geneitcs Laboratory, RIKEN.

Please note that the information was bioinformatically annotated and that this information may not reflect the most recent annotation of the reference sequence. You should carefully check the insert sequence provided by us to make sure that it matches the sequence you expect.
Gene Engineering Division has digested by restriction enzymes and sequenced a portion of the resource for verification. The result is shown below if available.

Please note that they are GENOMIC clones.

Entry vector

Catalog number Clone name Clone status
SPW009259 SpENT23C11 confirmation required
user_report - reference Toteva, T., Establishment of expression-state boundaries by Rif1 and Taz1 in fission yeast. Proc. Natl. Acad. Sci. U.S.A. 114 (5): 1093-1098 (2017). PMID 28096402.

FFH Vector (FLAG2-His6-tag clone)

Catalog number Clone name Clone status
SPW049259 SpFFH23C11 confirmation required

YFH Vector (YFP-FLAG-His6-tag clone)

Catalog number Clone name Clone status
SPW089259 SpYFH23C11 confirmation required

Reference of clones: Matsuyama, A., Nature Biotech., 24, 841-847 (2006).

Please visit Data Sheet for the information of vectors and sequence primers.


2023.11.01

pombe_clone_primer_ver2_2023Apr26.csv - GNP_filter3_pombe_html_191224.pl