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30/G03 - S. pombe Postgenome Database Clone

 Plasmid request
Clone ID at the S. pombe Postgenome Database 30/G03
NCBI Gene ID 2542144
Gene Symbol rif1
Protein Name telomere length regulator protein Rif1
Synonym SPAC6F6.17|tap1,tap11,SPAPJ736.01
RefSeq Protein
PomBase(1) SPAC6F6.17
KEGG(2) spo:SPAC6F6.17
Depositor-supplied data(3) 30G03
Note 13A:G / 1953T:C / 3658T:C(4)

(1) The PomBase, funded by the Wellcome Trust and is run by a consortium comprising the University of Cambridge, the European Bioinformatics Institute and University College London.

(2) The Kyoto Encyclopedia of Genes and Genomes of the Kanehisa Laboratories in the Bioinformatics Center of Kyoto University and the Human Genome Center of the University of Tokyo.

(3) Primer sequences for constructing an entry clone and known sequence variation are available at this site, the S. pombe Postgenome Database of the Chemical Geneitcs Laboratory, RIKEN.

(4) Remarks by the Depositor.

Please note that the information was bioinformatically annotated and that this information may not reflect the most recent annotation of the reference sequence. You should carefully check the insert sequence provided by us to make sure that it matches the sequence you expect.
Gene Engineering Division has digested by restriction enzymes and sequenced a portion of the resource for verification. The result is shown below if available.

Please note that they are GENOMIC clones.

Entry vector

Catalog number Clone name Clone status
SPW012147 SpENT30G03 available
Seq File
(by primer A)
Seq File
(by primer B)
Seq File
(by primer C)
PDF File
SpENT30G03a.seq SpENT30G03b.seq   SpENT30G03.pdf
user_report - reference Toteva, T., Establishment of expression-state boundaries by Rif1 and Taz1 in fission yeast. Proc. Natl. Acad. Sci. U.S.A. 114 (5): 1093-1098 (2017). PMID 28096402.

FFH Vector (FLAG2-His6-tag clone)

Catalog number Clone name Clone status
SPW052147 SpFFH30G03 confirmation required

YFH Vector (YFP-FLAG-His6-tag clone)

Catalog number Clone name Clone status
SPW092147 SpYFH30G03 confirmation required

Reference of clones: Matsuyama, A., Nature Biotech., 24, 841-847 (2006).

Please visit Data Sheet for the information of vectors and sequence primers.


2023.11.07

pombe_clone_primer_ver2_2023Apr26.csv - GNP_filter3_pombe_html_191224.pl