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40/E03 - S. pombe Postgenome Database Clone

 Plasmid request
Clone ID at the S. pombe Postgenome Database 40/E03
NCBI Gene ID 2541193
Gene Symbol dis2
Protein Name serine/threonine protein phosphatase PP1
Synonym SPBC776.02c|sds1,bws1
RefSeq Protein NP_596317.1
PomBase(1) SPBC776.02c
KEGG(2) spo:SPBC776.02c
Depositor-supplied data(3) 40E03
Note 1119T:C / 1192T:C(4)

(1) The PomBase, funded by the Wellcome Trust and is run by a consortium comprising the University of Cambridge, the European Bioinformatics Institute and University College London.

(2) The Kyoto Encyclopedia of Genes and Genomes of the Kanehisa Laboratories in the Bioinformatics Center of Kyoto University and the Human Genome Center of the University of Tokyo.

(3) Primer sequences for constructing an entry clone and known sequence variation are available at this site, the S. pombe Postgenome Database of the Chemical Geneitcs Laboratory, RIKEN.

(4) Remarks by the Depositor.

Please note that the information was bioinformatically annotated and that this information may not reflect the most recent annotation of the reference sequence. You should carefully check the insert sequence provided by us to make sure that it matches the sequence you expect.
Gene Engineering Division has digested by restriction enzymes and sequenced a portion of the resource for verification. The result is shown below if available.

Please note that they are GENOMIC clones.

Entry vector

Catalog number Clone name Clone status
SPW016099 SpENT40E03 confirmation required
user_report - reference Toteva, T., Establishment of expression-state boundaries by Rif1 and Taz1 in fission yeast. Proc. Natl. Acad. Sci. U.S.A. 114 (5): 1093-1098 (2017). PMID 28096402.

FFH Vector (FLAG2-His6-tag clone)

Catalog number Clone name Clone status
SPW056099 SpFFH40E03 confirmation required

YFH Vector (YFP-FLAG-His6-tag clone)

Catalog number Clone name Clone status
SPW096099 SpYFH40E03 confirmation required

Reference of clones: Matsuyama, A., Nature Biotech., 24, 841-847 (2006).

Please visit Data Sheet for the information of vectors and sequence primers.


2023.11.07

pombe_clone_primer_ver2_2023Apr26.csv - GNP_filter3_pombe_html_191224.pl