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20/F06 - S. pombe Postgenome Database Clone

 Plasmid request
Clone ID at the S. pombe Postgenome Database 20/F06
NCBI Gene ID 2542099
Gene Symbol uba3
Protein Name NEDD8 activating enzyme
Synonym SPAC24H6.12c
RefSeq Protein NP_592940.1
PomBase(1) SPAC24H6.12c
KEGG(2) spo:SPAC24H6.12c
Depositor-supplied data(3) 20F06
Note 99A:G / 336T:C / 501C:T / 820A:G / 1287T:C(4)

(1) The PomBase, funded by the Wellcome Trust and is run by a consortium comprising the University of Cambridge, the European Bioinformatics Institute and University College London.

(2) The Kyoto Encyclopedia of Genes and Genomes of the Kanehisa Laboratories in the Bioinformatics Center of Kyoto University and the Human Genome Center of the University of Tokyo.

(3) Primer sequences for constructing an entry clone and known sequence variation are available at this site, the S. pombe Postgenome Database of the Chemical Geneitcs Laboratory, RIKEN.

(4) Remarks by the Depositor.

Please note that the information was bioinformatically annotated and that this information may not reflect the most recent annotation of the reference sequence. You should carefully check the insert sequence provided by us to make sure that it matches the sequence you expect.
Gene Engineering Division has digested by restriction enzymes and sequenced a portion of the resource for verification. The result is shown below if available.

Please note that they are GENOMIC clones.

Entry vector

Catalog number Clone name Clone status
SPW008126 SpENT20F06 confirmation required

FFH Vector (FLAG2-His6-tag clone)

Catalog number Clone name Clone status
SPW048126 SpFFH20F06 available
Seq File
(by primer A)		 Seq File
(by primer B)		 Seq File
(by primer C)		 PDF File
SpFFH20F06a.seq SpFFH20F06b.seq   SpFFH20F06.pdf
user_report - reference Girdwood, D., Constitutively active Cullin-RING-Ligases fail to rescue loss of NEDD8 conjugation in Schizosaccharomyces pombe. FEBS Lett., 586 (10): 1522-1528 (2012). PMID 22673520.

YFH Vector (YFP-FLAG-His6-tag clone)

Catalog number Clone name Clone status
SPW088126 SpYFH20F06 confirmation required

Reference of clones: Matsuyama, A., Nature Biotech., 24, 841-847 (2006).

Please visit Data Sheet for the information of vectors and sequence primers.

2023.11.07

pombe_clone_primer_ver2_2023Apr26.csv - GNP_filter3_pombe_html_191224.pl