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07/D07 - S. pombe Postgenome Database Clone

 Plasmid request
Clone ID at the S. pombe Postgenome Database 07/D07
NCBI Gene ID 2539083
Gene Symbol rpb6
Protein Name DNA-directed RNA polymerase I, II and III subunit Rpb6
Synonym SPCC1020.04c|rpo15
RefSeq Protein NP_587956.1
PomBase(1) SPCC1020.04c
KEGG(2) spo:SPCC1020.04c
Depositor-supplied data(3) 07D07
Note 9C:T / 399T:deletion(4)

(1) The PomBase, funded by the Wellcome Trust and is run by a consortium comprising the University of Cambridge, the European Bioinformatics Institute and University College London.

(2) The Kyoto Encyclopedia of Genes and Genomes of the Kanehisa Laboratories in the Bioinformatics Center of Kyoto University and the Human Genome Center of the University of Tokyo.

(3) Primer sequences for constructing an entry clone and known sequence variation are available at this site, the S. pombe Postgenome Database of the Chemical Geneitcs Laboratory, RIKEN.

(4) Remarks by the Depositor.

Please note that the information was bioinformatically annotated and that this information may not reflect the most recent annotation of the reference sequence. You should carefully check the insert sequence provided by us to make sure that it matches the sequence you expect.
Gene Engineering Division has digested by restriction enzymes and sequenced a portion of the resource for verification. The result is shown below if available.

Please note that they are GENOMIC clones.

Entry vector

Catalog number Clone name Clone status
SPW002879 SpENT07D07 confirmation required

FFH Vector (FLAG2-His6-tag clone)

Catalog number Clone name Clone status
SPW042879 SpFFH07D07 confirmation required

YFH Vector (YFP-FLAG-His6-tag clone)

Catalog number Clone name Clone status
SPW082879 SpYFH07D07 confirmation required
user_report - reference Weston, L., Genome-wide screen for cell growth regulators in fission yeast. J. Cell Sci. 130 (12): 2049-2055 (2017). PMID 28476936.

Reference of clones: Matsuyama, A., Nature Biotech., 24, 841-847 (2006).

Please visit Data Sheet for the information of vectors and sequence primers.


2023.10.31

pombe_clone_primer_ver2_2023Apr26.csv - GNP_filter3_pombe_html_191224.pl