S. pombe ORFeome plasmid | Gene Engineering Division - DNA BANK

Schizosaccharomyces pombe ORFeome plasmids were deposited by Dr. Minoru Yoshida, Chemical Genetics Lagoratory, RIKEN; These consists of 4,900 entry plasmids. Plasmids are provided individually.

Establishing an entire protein assay system of “fission yeast”: Trends from genetic information to entire products in genomic analysis [2006.06.26 RIKEN Press release (pdf)]
Localization data is available at
S. pombe Postgenome Database

The Schizosaccharomyces pombe Postgenome Database (SPD) contains the results of systematic analysis of protein expression and localization based on fission yeast ORFeome cloning as well as basic information on genes and proteins.
The ORF collection currently covers 99.2% of the fission yeast protein-coding genes and pseudogenes that are reported by the Wellcome Trust Sanger Institute.

Distribution of plasmids

in Japanese

1. . Distribution of plasmids

1.1 Terms and conditions for distribution

In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the USER must cite the following literature(s). (Matsuyama et al., 2006, Nature Biotech., 24, 841-847.).
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the USER must state an acknowledgement to the DEPOSITOR.
When the USER wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the USER must obtain a prior written permission from the DEPOSITOR .
When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the USER’s name and his/her affiliation.

1.2 How to obtain plasmids

Plasmids are shipped as DNA solution in TE buffer in an individual tube.

Order Forms

Please specify ID of individual plasmids.

There are three kinds of plasmids as follows.
Entry plasmid (SpENT), Cat#. RDB 5820
FLAG2-His6-tagged ORF (SpFFH), Cat#. RDB 6106
YFP-FLAG-His6-tagged ORF (SpYFH), Cat#. RDB 6107
Please reffer Data Sheet for datail.

Please write plasmid ID in FormA, for example,
01/A01 SPBC1711.15c (SpENT)
01/A01 SPBC1711.15c (SpYFH)
01/B11 SPAC16A10.05c (SpYFH)
01/E01 SPCC191.04c (SpFFH)

Order Form

Order Form:
Please complete the form with your shipping information including your account number of an international courier
(FedEx. World Courier, TNT Express, DHL Global Forwarding and others).
See detail in Information of Request for Distribution

Note:
The resident of the European Economic Area (EEA) and China, please read Special distribution Information to Residents of the Foreign Countries

Order Form for Credit Card Payment. (Visa or Master Card only) [Word] [Example of order form]

Order Form for Bank Transfer Payment. [Word] [Example of order form]

Material Transfer Agreement for S. pombe ORFeome plasmids:
We would like to ask a signature of the Authorized Representative of a recipient institution.
Regarding description of specificpurpose in the Section 2(a):
Please write your research purpose in detail. We need description specifically how and for what purpose you are going to use the DNA Bank resource(s). If the information is considered insufficient, we may ask you to add more or rewrite it.
We can check whether the documents are filled out correctly or not in advance. Please email documents to us.

Please visit Ordering Information for further information of distribution and fees.

Send forms by regular mail or scanned PDF by e-mail:

The DNA Bank, RIKEN BioResource Research Center,
3-1-1 Koyadai, Tsukuba, Ibaraki 305-0074, Japan
E-mail: dnabank.brc@riken.jp

Distribution fee per clone (as of April 1st, 2023):
JPY 9,460 (For use in research for not-for-profit academic purpose).

The cost of shipping containers, dry ice (if required) and freight will be charged separately. These are not included in the fee.
For details for fee and payment, please visit Information of Request for Distribution.

DATA SHEET

1. Schizosaccharomyces pombe ORFeome plasmids

1.2 Plasmid information

Entry plasmids
Vector	pDONR201 (Kan^r), if not specified individually
Insert	PCR amplification of GENOMIC DNA of the wild-type strain JY3 Amplified with 5′-G GGG ACA AGT TTG TAC AAA AAA GCA GGC TCT CAT ATG-(template specific sequence)-3′ and 5′-GGG GAC CAC TTT GTA CAA GAA AGC TGG GTA-(template specific sequence)-3′ as primers.
Host	DH5alpha, E. coli

FLAG2-His6-tagged ORFs
Vector	pDUAL-FFH1c (Amp^r), if not specified individually
Insert	Corresponding gene was cloned from the entry plasmid to pDUAL-FFH1c (RDB06142) using the Gateway technology.
Host	DH5alpha, E. coli

YFH-tagged ORFs
Vector	pDUAL-YFH1c (Amp^r), if not specified individually
Insert	Corresponding gene was cloned from the entry plasmid to pDUAL-YFH1c (RDB06151) using the Gateway technology.
Host	DH5alpha, E. coli

1.2 Reference

ORFeome_Localizome_data.csv (1.2 MB; 2007.07.02), provided by the depositor of S. pombe ORFeome plasmid.

Reference

Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Horaoka Y., Horinouchi S., Yoshida M. (2006) ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe. Nature Biotech., 24, 841-847.(link)
A dataset including the cloning status of the ORFeome entry clones, protein expression levels, and localizome data is available. Please obtain “Supplementary Table 6” of Nat. Biotechnol. 24 (7): 841-847 (2006).
Bauer F, Matsuyama A, Candiracci J, Dieu M, Scheliga J, Wolf DA, Yoshida M, Hermand D. (2012) Translational control of cell division by elongator. Cell Rep. 1 (5): 424-433.
Bauer F, Matsuyama A, Yoshida M, Hermand D. (2012) Determining proteome-wide expression levels using reverse protein arrays in fission yeast. Nat. Protoc. 7 (10): 1830-1835.
Users’ publication Informaion site
Entry plasmid
FLAG2-His6-tagged ORF
YFP-FLAG-His6-tagged ORF .

Related information

pDUAL vector series
pDUAL2 vector series
pREP vector series
Please find vectors.
Plasmid is shipped as DNA solution in TE buffer in an individual tube.
Please specify ID of individual plasmid.

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2025.05.03 (T.M. GRP0054e)