ATP imaging

Development of fluorescence lifetime biosensors for ATP, cAMP, citrate, and glucose using the mTurquoise2-based platform.
Zhong, C., Arai, S., Okada, Y.
Cell Rep. Methods 4 (11): 100902 (2024). PubMed PMID 39561716.

Catalog number	Name of Resource	Description
RDB20448	pcDNA3.1(-)-qmTQ2-ATP	Mammalian expression of qmTQ2-ATP, a cyan fluorescent FLIM biosensor for ATP imaging.
RDB20447	pRSETA-qmTQ2-ATP	Bacterial expression of N-terminal His-tagged qmTQ2-ATP, a cyan fluorescent FLIM biosensor for ATP imaging.

Diversity in ATP concentrations in a single bacterial cell population revealed by quantitative single-cell imaging.
Yaginuma, H., Kawai, S., Tabata, K.V., Tomiyama, K., Kakizuka, A., Komatsuzaki, T., Noji, H., Imamura, H.
Sci. Rep. 4: 6522 (2014). PubMed PMID 25283467.

Catalog number	Name of Resource	Description
RDB17766	pN1-QUEEN7mu	Low concentration ATP measurement
RDB17783	pN1-ITPKA-Q7mu	Low concentration ATP measurement, actin
RDB17777	pN1-COX-Q7mu	Low concentration ATP measurement, mitochondria matrix
RDB17784	pN1-TOMM20-Q7mu	Low concentration ATP measurement, mitochondria outer membrane
RDB17771	pN1-NLS-Q7mu	Low concentration ATP measurement, nucleus
RDB17758	pN1-PTS2-Q7mu	Low concentration ATP measurement, peroxisome
RDB17773	pN1-SSTRv2-Q7mu	Low concentration ATP measurement, primary cilia
RDB17760	pN1-Tubbeta5-Q7mu	Low concentration ATP measurement, Tubulin
RDB17762	pT2P-UB-Q7mu	Low concentration ATP measurement, Tol2 transposon
RDB17761	pT2P-EF-Q7mu	Low concentration ATP measurement, Tol2 transposon
RDB17780	pT2MP.EF-ITPKA-Q7mu	Low concentration ATP measurement, actin, Tol2 transposon
RDB17772	pT2MP.EF-TOMM-Q7mu	Low concentration ATP measurement, mitochondria outer membrane, Tol2 transposon
RDB17770	pRS-QUE7mu	Low concentration ATP measurement
RDB17767	pN1-QUE2m	QUEEN ATP sensor for 25degC
RDB17781	pN1-ITPKA-Q2m	QUEEN ATP sensor for 25degC, actin
RDB17779	pN1-COX-Q2m	QUEEN ATP sensor for 25degC, mitochondria matrix
RDB17775	pN1-NLS-Q2m	QUEEN ATP sensor for 25degC, nucleus
RDB17769	pT2MP.EF-Q2m	QUEEN ATP sensor for 25degC, Tol2 transposon
RDB17763	pT2MP.UB-Q2m	QUEEN ATP sensor for 25degC, Tol2 transposon
RDB17785	pT2MP.EF-NLS-Q2m	QUEEN ATP sensor for 25degC, nucleus, Tol2 transposon
RDB17768	pN1-QNA	Negative control for ATP measurement
RDB17755	pN1-QUEEN7mu R122K R126K	Negative control for ATP measurement
RDB17782	pN1-ITPKA-QNA	Negative control for ATP measurement, actin
RDB17778	pN1-COX-QNA	Negative control for ATP measurement, mitochondria matrix
RDB17757	pN1-TOMM20-QNA	Negative control for ATP measurement, mitochondria outer membrane
RDB17774	pN1-NLS-QNA	Negative control for ATP measurement, Nucleus
RDB17756	pN1-PTS2-QNA	Negative control for ATP measurement, Peroxisome
RDB17759	pN1-Tubbeta5-QNA	Negative control for ATP measurement, Tubulin
RDB17764	pRSET-QNA	Negative control for ATP measurement, G3C mutation fixed (2019.11.19)
RDB17765	pT2MP.EF-QNA	Negative control for ATP measurement, Tol2 transposon
RDB17776	pT2MP.EF-TOMM-QNA	Negative control for ATP measurement, mitochondria outer membrane, Tol2 transposon

(GRP0066e 2020.02.05 T.M.)

2024.12.05