G1 cdks and cdk inhibitors
Somatic cell cycles consist of alternating DNA synthetic (S) and mitotic (M) phases, separated by gap phases (G1 and G2). Mammalian cells respond to extracellular mitogens and antiproliferative cytokines from the time that they exit mitosis (vertical arrow at top) until they reach the restriction point, after which they can complete the cell division cycle in the absence of extracellular growth factors. Cyclin D-dependent kinases accumulate in response to mitogenic signals and initiate the phosphorylation of Rb, a process that is completed by cyclin E-cdk2. Once cells enter S phase, cyclin E is degraded and cyclin A enters into complexes with cdk2. INK4 proteins oppose the activities of the various cyclin D-dependent kinases, whereas Cip/Kip proteins specifically inhibit cyclin E-cdk2 and cyclin A-cdk2. (Contributed by Dr. Koji Nakade)
Reporters
List of gene resources
| Locus | RDB no. | Type | Name of clone | Running title |
|---|---|---|---|---|
| CDKN1B | 5508 | DNA clone | pKM2L-phKIP1 | Promoter Bank clone, Human p27 cyclin-dependent kinase inhibitor (kip1) promoter |
| CDKN1B | 4645 | ShuttleVector | pAxCALNLhp27(forward) | Shuttle vector to generate rAd expressing human CDKN1B |
| CDKN1B | 4642 | ShuttleVector | pAxCALNLhp27(reverse) | Shuttle vector to generate rAd expressing human CDKN1B |
| CDKN1B | 2069 | ShuttleVector | pAxCAhp27 (As) | Shuttle vector to generate rAd expressing human CDKI p27 |
| CDKN1B | 2011 | ShuttleVector | pAxCAhp27 | Shuttle vector to generate rAd expressing human CDKI p27 |
| CDKN1B | 2010 | Recombinant Adenovirus | AxCAhp27 | Recombinant adenovirus expressing human CDKI p27 regulated by CAG promoter |
| Cdkn1b | 2856 | DNA clone | HA-P27 | Expression vector of HA-tagged mouse p27/Kip1 |
| CDKN1A | 5893 | DNA clone | pKM2L-phP21 | Promoter Bank clone, Human p21 cyclin dependent kinase inhibitor/WAF1/CIP1 promoter |
| CDKN1A | 3576 | ShuttleVector | pAxCALNLhp21(forward) | Shuttle vector to generate rAd harboring Sugano full-length cDNA clone |
| CDKN1A | 1999 | ShuttleVector | pAxCAhp21 | Shuttle vector to generate rAd expressing human CDKI p21 |
| Cdk4 | 6971 | H3147D06 | Cdk4, NIA/NIH Mouse 15K cDNA Clone (full CDS) | |
| CDK2 | 5250 | ShuttleVector | pAxCALNLhCDK2(reverse) | Shuttle vector to generate rAd expressing human CDK2 |
| CDK2 | 5249 | ShuttleVector | pAxCALNLhCDK2(forward) | Shuttle vector to generate rAd expressing human CDK2 |
| CDK2 | 4061 | ShuttleVector | pAxCALNLhCDK2 | Shuttle vector to generate rAd harboring Sugano full-length cDNA clone |
| CDK2 | 3492 | ShuttleVector | pAxCALNLhCDK2(forward) | Shuttle vector to generate rAd harboring Sugano full-length cDNA clone |
| CDK2 | 3472 | ShuttleVector | pAxCALNLhCDK2(forward) | Shuttle vector to generate rAd harboring Sugano full-length cDNA clone |
| Cdk2 | 6947 | H3117F10 | Cdk2, NIA/NIH Mouse 15K cDNA Clone (full CDS) | |
| CDC25C | 5908 | DNA clone | pKM2L-phCDC25C(2) | Promoter Bank clone, Human cdc25C promoter |
| CDC25C | 5841 | ShuttleVector | pAxit-phCDC25C-rLuc (R) | Shuttle vector to generate recombinant adenovirus harboring renilla luciferase reporter. |
| CDC25C | 5840 | ShuttleVector | pAxit-phCDC25C-rLuc (F) | Shuttle vector to generate recombinant adenovirus harboring renilla luciferase reporter. |
| CDC25C | 5468 | DNA clone | pKM2L-phCDC25C | Promoter Bank clone, Human cdc25C promoter |
| CDC25B | 2668 | ShuttleVector | pAxCALNLhCDC25B (reverse) | Shuttle vector to produce rAd expressing human CDC25B |
| CDC25B | 2667 | ShuttleVector | pAxCALNLhCDC25B (forward) | Shuttle vector to produce rAd expressing human CDC25B |
| CDC25B | 2334 | ShuttleVector | pAxCAhCDC25B (reverse) | Shuttle vector to produce rAd expressing human CDC25B |
| CDC25B | 2333 | ShuttleVector | pAxCAhCDC25B (forward) | Shuttle vector to produce rAd expressing human CDC25B |
| CDC25B | 1203 | DNA clone | CDC25Hu2 | Human cdc 25B cDNA |
| CDC25A | 5476 | DNA clone | pKM2L-phCDC25A | Promoter Bank clone, Human cdc25A promoter |
| CDC25A | 4653 | ShuttleVector | pAxCALNLhCDC25A(forward) | Shuttle vector to generate rAd expressing human CDC25A |
| CDC25A | 4646 | ShuttleVector | pAxCALNLhCDC25A(reverse) | Shuttle vector to generate rAd expressing human CDC25A |
| cdc2 | 1570 | DNA clone | pIBI delta Xe-cdc2-33R | Xenopus cdc2 (kinase-deficient) cDNA |
| cdc2 | 1569 | DNA clone | pIBI delta Xe-cdc2 | Xenopus cdc2 cDNA |
| CCNH | 6416 | DNA clone | pCMFlag_hsCCNH | Expression vector of human CCNH |
| CCNH | 4093 | ShuttleVector | pAxCALNLhCyclin H | Shuttle vector to generate rAd harboring Sugano full-length cDNA clone |
| CCNH | 3510 | ShuttleVector | pAxCALNLhCyclin H(forward) | Shuttle vector to generate rAd harboring Sugano full-length cDNA clone |
| CCND3 | 4589 | DNA clone | SEREX clone NGO-Br-20 (ID 859) #1 | SEREX clone NGO-Br-20 (ID 859) #1 |
| CCNB2 | 6415 | DNA clone | pCMFlag_hsCCNB2 | Expression vector of human CCNB2 |
| CCNB2 | 5448 | ShuttleVector | pAxCALNLhCCNB2(reverse) | Shuttle vector to generate rAd harboring CCNB2 Sugano full-length cDNA clone |
| CCNB2 | 5447 | ShuttleVector | pAxCALNLhCCNB2(forward) | Shuttle vector to generate rAd harboring CCNB2 Sugano full-length cDNA clone |
| CCNB2 | 5430 | ShuttleVector | pAxCALNLhCCNB2(forward) | Shuttle vector to generate rAd harboring CCNB2 Sugano full-length cDNA clone |
| CCNB2 | 5425 | ShuttleVector | pAxCALNLhCCNB2(reverse) | Shuttle vector to generate rAd harboring CCNB2 Sugano full-length cDNA clone |
| CCNB2 | 5424 | ShuttleVector | pAxCALNLhCCNB2(forward) | Shuttle vector to generate rAd harboring CCNB2 Sugano full-length cDNA clone |
| CCNB2 | 5367 | ShuttleVector | pAxCALNLhCCNB2(reverse) | Shuttle vector to generate rAd harboring CCNB2 Sugano full-length cDNA clone |
| CCNB2 | 5366 | ShuttleVector | pAxCALNLhCCNB2(forward) | Shuttle vector to generate rAd harboring CCNB2 Sugano full-length cDNA clone |
| CCNB2 | 4098 | ShuttleVector | pAxCALNLhCCNB2 | Shuttle vector to generate rAd harboring Sugano full-length cDNA clone |
| CCNB2 | 3515 | ShuttleVector | pAxCALNLhCCNB2(forward) | Shuttle vector to generate rAd harboring Sugano full-length cDNA clone |
| CCNA2 | 6665 | DNA clone | pCMFlag_hsCCNA2 | Expression vector of human CCNA2/Cyclin A2 |
| CCNA2 | 4162 | ShuttleVector | pAxCALNLhCyclin A2 | Shuttle vector to generate rAd harboring Sugano full-length cDNA clone |
| CCNA2 | 3595 | ShuttleVector | pAxCALNLhCyclin A2 (forward) | Shuttle vector to generate rAd harboring Sugano full-length cDNA clone |
| CCNA1 | 5482 | DNA clone | pKM2L-phCYCA1 | Promoter Bank clone, Human cyclin A1 promoter |
| CCNA1 | 3557 | ShuttleVector | pAxCALNLhCCNA1(forward) | Shuttle vector to generate rAd harboring Sugano full-length cDNA clone |
| CCNA1 | 3534 | ShuttleVector | pAxCALNLhCCNA1(forward) | Shuttle vector to generate rAd harboring Sugano full-length cDNA clone |
| BCCIP | 2617 | Recombinant adenovirus | AxCALNLhTOK-1 alpha | recombinant adenovirus expressing human TOK-1 alpha |
| BCCIP | 2346 | Recombinant Adenovirus | AxCAhTOK-1 alpha | Recombinant adenovirus expressing human TOK-1 alpha |
(GSB0007e, T.M. 2004.04.05)
2018.09.24
