Resource data sheet
DNA Bank Top
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pBKV525 (#RDB01019)

Human BK virus 525

Alternative name Human BK virus 525
Clone info. Human DNA virus BKV 525
Vector backbone pBR325 (Plasmid)
Size of vector backbone 6.0 kb
Selectable markers Amp^r
Gene/insert name Hum_Polyoma BKV transforming gene Genomic DNA
Depositor|Developer Soeda, Eiichi |
 
Additional map

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB01019 pBKV525 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pBKV525 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB01019).

Reference section:

Watanabe, S., Soeda, E., Uchida, S., Yoshiike, K., DNA rearrangement affecting expression of the BK virus transforming gene. J. Virol., 51, 1-6 (1984). PMID 6328028.

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB12317_A3Kj.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer:
Sequence file: RDB12317_A3Kja.seq check
>01019_12317_A3Kj_Amp_R_G10_19.ab1
    1 CTCCATTTTC ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT
   61 TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC
  121 CACCTGACGT CTAAGAAACC ATTATTATCA TGACATTAAC CTATAAAAAT AGGCGTATCA
  181 CGAGGCCCTT TCGTCTTCAA GAATTCTCAT GTTTGACAGC TTATCATCGA TAAGCTTTTC
  241 TCATTAAGGG AAGATTTCCC CAGGCAGCTC TTTCAAGGCC TAAAAGGTCC ATGAGCTCCA
  301 TGGATTCTTC CCTGTTAAGA ACTTTATCCA TTTTTGCAAA AATTGCAAAA GAATAGGGAT
  361 TTCCCCAAAT AGTTTTGCTA GGCCTCAGAA AAAGCCTCCA CACCCTTACT ACTTGAGAGA
  421 AAGGGTGGAG GCAGAGGCGG CCTCGGCCTC TTATATATTA TAAAAAAAAA GGCCACAGGG
  481 AGGAGCTGCT TACCCATGGA ATGCAGCCAA ACCATGACCT CAGGAAGGAA AGTGCATGAC
  541 TGGGCAGCCA GCCATGACCT CAGGAAGGAA AGTGCATGAC TGGGCAGCCA GCCAGTGGCA
  601 GTTAATAGTT AAACTGGACA AAGGAAAGTG CATGACTGGG CAGCCAGCCA TGACCTCAGG
  661 AAGGAAAGTG CATGACTGGG CAGCCAGCCA GTGGCAGTTA ATAGTTAAAC TGGACAAAGG
  721 CCATGGTTCT GCGCCAGCTG TCACGACAAG CTTTAATGCG GTAGTTTATC ACAGTTAAAT
  781 TGCTAACGCA GTCAGGCACC GTGTATGAAA TCTAACAATG CGCTCATCGT CATCCTCGGC
  841 ACCGTCACCC TGGATGCTGT AGGCATAGGC TTGGTTATGC CGGTACTGCC GGGCCTCTTG
  901 CGGGATATCG TCCATTCCGA CAGCATCGCC AGTCACTATG GCGTGCTGCT AGCGCTATAT
  961 GCGTTGATGC AATTTCTATG CGCACCCGTT CTCGGAGCAC TGTCCGACCG CTTTGGGCCG
 1021 CCGCCCAGTC CTGCTCGCTT CGCTACTTGG AGCCACTATC GACTACGCGA TCATGGCGAC
 1081 CACACCCGTC CCTGTGATCT CTACGCCGGG ACGCATCGTG GTCGCCATCA CCGGCGCCAC
1141 AGGTGCGATG CCTGCGCTTA TTATCGCCGA ACCTTCACCG AAATGGGGGA GAT
//
Primer:
Sequence file: RDB12317_A3Kjb.seq check
>01019_12317_A3Kj_pBR322_BamHI_R_D08_11.ab1
    1 TGGATTTAGG GCCCCCATGG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC CCAATCGAGC
   61 AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT
  121 TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG
  181 ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC
  241 AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA
  301 CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTGTCGTG ACAGCTGGCG
  361 CAGAACCATG GCCTTTGTCC AGTTTAACTA TTAACTGCCA CTGGCTGGCT GCCCAGTCAT
  421 GCACTTTCCT TCCTGAGGTC ATGGCTGGCT GCCCAGTCAT GCACTTTCCT TTGTCCAGTT
  481 TAACTATTAA CTGCCACTGG CTGGCTGCCC AGTCATGCAC TTTCCTTCCT GAGGTCATGG
  541 CTGGCTGCCC AGTCATGCAC TTTCCTTCCT GAGGTCATGG TTTGGCTGCA TTCCATGGGT
  601 AAGCAGCTCC TCCCTGTGGC CTTTTTTTTT ATAATATATA AGAGGCCGAG GCCGCCTCTG
  661 CCTCCACCCT TTCTCTCAAG TAGTAAGGGT GTGGAGGCTT TTTCTGAGGC CTAGCAAAAC
  721 TATTTGGGGA AATCCCTATT CTTTTGCAAT TTTTGCAAAA ATGGATAAAG TTCTTAACAG
  781 GGGAAGAATC CATGGAGCTC ATGGACCTTT TAGGCCCTTT GAAAGAGCTG CCTGGGGAAA
  841 TCTTCCCTTA ATGAGAAAAA GCTTATCGAT GATAAGCTGT TCAAACATGA GAAATTCTTG
  901 AAGACGAAAG GGCCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG
  961 TTTCTTAAGA CGTCAGGTGG CACTTTTTCG GGAAATGTGC GCGGAACCCC CTATTTGTTT
 1021 ATTTTTCTAA ATACATTTCA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT
 1081 TTCATAATAT TGAAAGGAAG AAGTATGAGT ATTTCAACAT TTCCGTGTCG CCTTATTCCC
 1141 TTTTTTGCGG CAATTTGCCT TCCTGATTTT GCTCCACCCA AGAAACGCCT GGGGTAAAGC
1201 TAAAAGAAAT GGCCTCTGTG CAG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Watanabe, S., DNA rearrangement affecting expression of the BK virus transforming gene. J. Virol., 51, 1-6 (1984). PMID 6328028.