Resource data sheet
DNA Bank Top
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pBKV501 (#RDB01020)

Human BK virus 501

Alternative name Human BK virus 501
Clone info. Human DNA virus BKV 501
Vector backbone pBR325 (Plasmid)
Size of vector backbone 6.0 kb
Selectable markers Amp^r
Gene/insert name Hum_Polyoma BKV transforming gene Genomic DNA
Depositor|Developer Soeda, Eiichi |
 
Additional map

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB01020 pBKV501 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pBKV501 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB01020).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB12318_A3Kj.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer:
Sequence file: RDB12318_A3Kja.seq check
>01020_12318_A3Kj_Amp_R_H10_22.ab1
    1 TTCCCCTTTT TTAAATTTAT TGGAAGCCTT TTATCCAGGG TTATTGTCTC ATGAGCGGAT
   61 ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA
  121 AAGTGCCACC TGACGTCTAA GAAACCATTA TTATCATGAC ATTAACCTAT AAAAATAGGC
  181 GTATCACGAG GCCCTTTCGT CTTCAAGAAT TCTCATGTTT GACAGCTTAT CATCGATAAG
  241 CTTGTCGTGA CAGCTGGCGC AGAACCATGG CCTTTGTCCA GTTTAACTGG GGACAAGGCC
  301 AAGATTCCTA GGCTCGCAAA ACATGTCTGT CGGCGGGGTT TCACTATTAA CTGCCACTGG
  361 CTGGCTGCCC AGTCATGCAC TTTCCTTCCT GAGGTCATGG TTTGGCTGCA TTCCATGGGT
  421 AAGCAGCTCC TCCCTGTGAG TCATGCACTT TCCTTCCTGA GGTCATGGTT TGGCTGCATT
  481 CCCCTGTGAG TAATGCACTT TCCTTCCTGA GGTCATGGTT TGGCTGCATT CCATGGGTAA
  541 GCAGCTCCTC CCTGTGGCCT TTTTTTTTAT AATATATAAG AGGCCGAGGC CGCCTCTGCC
  601 TCCACCCTTT CTCTCAAGTA GTAAGGGTGT GGAGGCTTTT TCTGAGGCCT AGCAAAACTA
  661 TTTGGGGAAA TCCCTATTCT TTTGCAATTT TTGCAAAAAT GGATAAAGTT CTTAACAGGG
  721 AAGAATCCAT GGAGCTCATG GACCTTTTAG GCCTTGACAG AGCTGCCTGG GGAAATCTTC
  781 CCTTAATGAG AAAAAGCTTT AATGCGGTAG TTTATCACAG TTAAATTGCT AACGCAGTCA
  841 GGCACCGTGT ATGAAATCTA AACAATGCGC TTCATCGTCA TCCCTCGGCA CCGTCACCCC
  901 TGGAATGCTG TAAGGCTTAG GCTTGGTTAT GCCCGGTACT GCCGGGCCCT CTTGCGGGGA
  961 TTATCGTCAT TCCCGACAGG CATCGCCAGT TCACTATGGC GTGCTTGCTA GCGCTATATG
 1021 CGTGATGCAA ATTCCTATGC GCCACCCGGT TCTCGGAGCA CTGTCCGACC GCTTTGGACC
 1081 GCCGCCCAGT CCTGGCTTCG CTTCCTTACT TGGACCCACT ATCGACTACC CGGAATCAAT
 1141 GGGCGGACCA CACCCGGTCC TTGAGTGGAT CTCCTCTATC ACGCCCAGGG AAGGCAATTC
 1201 TTGGTCCGGA CATTCCACGC GGCGCCTACC AGGAGAGTGC GGGGAATTGC TAGTGCAGTG
//
Primer:
Sequence file: RDB12318_A3Kjb.seq check
>01020_12318_A3Kj_pBR322_BamHI_R_E08_14.ab1
    1 CAATTTTTGG TCGCCAATGA ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA
   61 GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT
  121 GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA
  181 CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA
  241 GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC
  301 TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTTTCTCAT TAAGGGAAGA
  361 TTTCCCCAGG CAGCTCTTTC AAGGCCTAAA AGGTCCATGA GCTCCATGGA TTCTTCCCTG
  421 TTAAGAACTT TATCCATTTT TGCAAAAATT GCAAAAGAAT AGGGATTTCC CCAAATAGTT
  481 TTGCTAGGCC TCAGAAAAAG CCTCCACACC CTTACTACTT GAGAGAAAGG GTGGAGGCAG
  541 AGGCGGCCTC GGCCTCTTAT ATATTATAAA AAAAAAGGCC ACAGGGAGGA GCTGCTTACC
  601 CATGGAATGC AGCCAAACCA TGACCTCAGG AAGGAAAGTG CATGACTCAC AGGGGAATGC
  661 AGCCAAACCA TGACCTCAGG AAGGAAAGTG CATGACTCAC AGGGAGGAGC TGCTTACCCA
  721 TGGAATGCAG CCAAACCATG ACCTCAGGAA GGAAAGTGCA TGACTGGGCA GCCAGCCAGT
  781 GGCAGTTAAT AGTGAAACCC CGCCGACAGA CATGTTTTGC GAGCCTAGGA ATCTTGGCCT
  841 TGTCCCCAGT TAAACTGGAC AAAGGCCATG GTTCTGCGCC AGCTGTCACG ACAAGCTTAT
  901 CGATGATAAG CTGTCAAACA TGAGAATTCT TGAAGACGAA AGGGCCTCGT GATACGCCTA
  961 TTTTTATAGG TTAATGTCAT GATAATAATG GGTTTCTTAG ACGTCAGGTG GCACTTTTCG
 1021 GGGAAATGTG CGCGGAAACC CCTATTTGTT ATTTTTTTCT AAAATACAAT TCAAATATGT
 1081 ATCCGCTCAT GAGACCATAA CCTGATAAAT GCTTCATTAT ATTGGAAACG GAAGAGTATG
1141 AGTATCCACA TTCCGTGTCG CCTTATATTC CCATTTTTTT TGCGGGACA
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

reference Weiher, H., Multiple point mutations affecting the simian virus 40 enhancer. Science, 219, 626-631 (1983). PMID 6297005.