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pH-2K^b (C1-4-1) (#RDB01048)

Mouse H-2Kb genome clone

Alternative name Mouse H-2K^b genome clone
Clone info. Mouse B6 mice spleen H-2K^b gene
Vector backbone pBR327 (Plasmid)
Size of vector backbone 3.27 kb
Selectable markers Amp^r, Tet^r
Gene/insert name Mouse H-2Kb antigen Genomic DNA
Depositor|Developer Yokoyama, Kazunari |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Proc. Natl. Acad. Sci. USA, 79, 3270-3274 (1982). J. Biol. Chem., 270, 2327-2336 (1995). Nucleic Acids Res., 18, 4185-4190 (1990)).
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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Proc. Natl. Acad. Sci. USA, 79, 3270-3274 (1982). J. Biol. Chem., 270, 2327-2336 (1995). Nucleic Acids Res., 18, 4185-4190 (1990))。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB01048 pH-2K^b (C1-4-1) DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pH-2K^b (C1-4-1) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB01048).

Reference section:

Tang, X., Li, H.O., Sakatsume, O., Ohta, T., Tsutsui, H., Smit, A.F., Horikoshi, M., Kourilsky, P., Israel, A., Gachelin, G., et al., Cooperatively between an upstream TATA-like sequence and a CAA repeated element mediates E1A-dependent negative repression of the H-2Kb class I gene. J. Biol. Chem., 270, 2327-2336 (1995). PMID 7836466.
Ozawa, K., Saka, F., Kitabayashi, I., Imai, T., Soeda, E., Israel, A., Gachelin, G., Yokoyama, K., Comparison of the promoter regions of H-2Kb and H-2Kbm1 class I MHC genes. Nucleic Acids Res., 18, 4185-4190 (1990). PMID 2377459.
Reyes, A.A., Schöld, M., Itakura, K., Wallace, R.B., Isolation of a cDNA clone for the murine transplantation antigen H-2Kb. Proc. Natl. Acad. Sci. USA, 79, 3270-3274 (1982). PMID 6954478.

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB12804_A4F5.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: Amp_R
Sequence file: RDB12804_A4F5a.seq check
>01048_12804_A4F5_Amp_R_A10_01.ab1
    1 TTCTTTTTCA TATTATTGAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT
   61 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA
  121 CCTGACGTCT AAGAAACCAT TATTATCATG ACATTAACCT ATAAAAATAG GCGTATCACG
  181 AGGCCCTTTC GTCTTCAAGA ATTCTTGCTG CTTCTGCAGT GTGATGATTG GCAGAGTCGA
  241 GTGAAAGTCT CTGGATATGT GAGCTGATAC AGACTCGGGT GGTGTTTTGC TGAGGCAAGT
  301 CTCATGGAAG GCAAGACTCG AGTGAGGACA CGTGATGTTT GGAGGGAGTA TAATGAGACA
  361 CAATGGACAG TGATGGTGGG CTCGCATAGC TTGCTTTGCA ATGCTACCGT GGTCTCAAGT
  421 CTTCATCTTC ATTGATCTTC ACTTTGTTGA GAGTCATGGC AGAGAACTCC TGGTCATTCC
  481 TGCTGACTCG GGCCGATTTG GTGGAGGCCT GGCAGTTTCT GCTGGGTCAT GCCATAGCCG
  541 ATGATTTGGG CTTGCTATCC TGACACTGCA GACCTGGACT GCTGGTATCC TGACAACTGG
  601 AGATTGAAAT TGCCCCAAGG AACTACTTCT AAACAGCAAA CAGCTGAGCC TCTTGGCTGC
  661 TTCTGCATCG AGCTGCTATT GCTGATTCTT GTGAACTGAT ATCCTGACAA TGCAGATTGG
  721 ATTTGCTTCA AAGAATCATT TCTAAACAGC TGAACTAATC CCATATCCTC ATATCCTTTT
  781 TATTCTCTAC TTCTGGTGGG TGGTGAGCTA TAAATGAGGT TAAGCATTTA AGAACCCTTA
  841 TTAAAAATGG GTTTAGAAAA ATCTAAGCCA ATTATCTCAC CTGAGGATGT GCACCTGAGA
  901 ATTTAACAAC AACAACAACA AAAAAAAAAA TCACATCCTA CACCTGAGAC CCCTGGGAAG
  961 AGAAACTGGA GAGAAAGGAG AAGCAGAAAC CACCACAGTA GCATGGGACA GGGAGAAACC
 1021 CAGGGGAGCG TTTAACATGG CATCATTAGT AGCTAAAACG AAATGGGCTG AAGAGGAAAT
 1081 GGGGTAAAGT GGTCTTCTGG CACTTGGGCA CCAATTAAAA GATCCCCTTA AGGTTTGGGA
1141 GGTTCCCTCC TA
//
Primer: pBR_EcoRI_R
Sequence file: RDB12804_A4F5b.seq check
>01048_12804_A4F5_pBR_EcoRI_R_B10_04.ab1
    1 GCGTAGCCGG GGATGAAGAA TGGGGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC
   61 GTTAGCAATT TAACTGTCCC TTAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA
  121 ACATGAGAAT TCTGGTCAGT TAGAGGAACT CTGGGAAGAG AGAAGCATGG GGGATTTGTC
  181 ACACGGGCTC ATAGAAAGTG GTATGTGTGA AACTGAGGAG AGGTGACCAG TCACATGGCA
  241 GAACTTAGAA TAGTATGAAT GGGATAACTG AATTATGAGA TAGCTGGAAA CAGGCCTAGC
  301 ATAAAGCATA GGCATTCAAA AACAAATGTA AGTCTGCCGT CATTATTCAG AGCAAGGGAG
  361 GACAAGCAGG AATCTCCGAC AGATAACAGG AGCAGGAGCA AGCAGACACA GAGCAAGCCT
  421 TTCCTTCTTC CATGTCTTCA TGTAGGCTTC CAGCAGAAGG CGTGGCCCAG ATCAGAAAGT
  481 GGGAGGGGCT CCCGCTCAAT TATTTTGGAT TACAAAGGGG GAGGGGCTCC CGCTCAAATA
  541 TTTTGGATTA CAAAGGGGGA GGGGCTCCTG CTCAAATATT CTGGATTACA GGTGTGTCTT
  601 CCTACCTCAA AGAAAAGGGT CAGAAATTGA TCTTCCCACT TCAAATTAAG CAAAAACGCC
  661 TCACAGATGG TGTCCTCCAT TTTGAGGTTT TAGTTAGCCC CACATGTAGC TGGCATCAAA
  721 GAACCAACAT CATGGTCCCT GTCCGACTTC TGGAACTGCT CTGTTTGGGG CTTTGCAGCT
  781 CCTTTTAGCA CTCATGACTC TGCCTCCTGC CTCCCCTCTT ACCGGGAGTT TTCTTTCCAC
  841 GGGTGGAAAA GCACGTAAAG TTCTTTGGGA TGTAAGTTTG ATGAGAGCAG GGCAGCCTGA
  901 GACCTGAAAG CCCTGGAGAA GTGCACACAG GAAGGAGGCC CTGGGGGCAG CTCAGCAGCC
  961 CTGGATCCCC TTTTACCCTT CAGCTCTGTG GGCTCAGACG CTGTTCCTCT TTTGTTTCCT
 1021 TCCACCAGGG CGGCGCTGTT GCCCAAAGAA TGTGATTTCT TCCCTTGTCA TCCTTCAACA
 1081 GGTGAAGACC CTGCAGGTTC TTGGCCAGTG GGGAGAAAGA ATTGGGGAAC GGGGAACACC
1141 TTTTCCTTCT TGCAGGATTG CTCTGATATT GCGCTTACTC ACAG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Tang, X., Cooperatively between an upstream TATA-like sequence and a CAA repeated element mediates E1A-dependent negative repression of the H-2Kb class I gene. J. Biol. Chem., 270, 2327-2336 (1995). PMID 7836466.
original Ozawa, K., Comparison of the promoter regions of H-2Kb and H-2Kbm1 class I MHC genes. Nucleic Acids Res., 18, 4185-4190 (1990). PMID 2377459.
original Reyes, A.A., Isolation of a cDNA clone for the murine transplantation antigen H-2Kb. Proc. Natl. Acad. Sci. USA, 79, 3270-3274 (1982). PMID 6954478.