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hCD4 (#RDB01137)

Human OKT4 cDNA

Alternative name Human OKT4 cDNA
Clone info. Human CD4 cDNA from human male peripheral blood lymphocytes cDNA library
Vector backbone pBluescript II SK (+) (Plasmid)
Size of vector backbone 3.0 kb
Selectable markers Amp^r
Gene/insert name Human CD4 cDNA
Depositor|Developer Nakauchi, Hiromitsu |
 
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB01137 hCD4 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The hCD4 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB01137).

Reference section:

Tokito, S., Kishi, S., Yamamoto, R., Takenaka, T., Nakauchi, H., Single amino acid substitution in the V3 domain of CD4 is responsible for OKT4 epitope deficiency. Immunogenetics, 34, 208-210 (1991). PMID 1716607. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB12826_A4Gi.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40
Sequence file: RDB12826_A4Gia.seq check
>01137_12826_A4Gi_M13(-40)_A10_01.ab1
    1 CAGCCAGTAG CGCGCGTATA CGACTCACTA TAGGGCGAAT TGGGTACCGG GCCCCCCCTC
   61 GAGGTCGACG GTATCGATAA GCTTGATGGC AAGGCCACAA TGAACCGGGG AGTCCCTTTT
  121 AGGCACTTGC TTCTGGTGCT GCAACTGGCG CTCCTCCCAG CAGCCACTCA GGGAAAGAAA
  181 GTGGTGCTGG GCAAAAAAGG GGATACAGTG GAACTGACCT GTACAGCTTC CCAGAAGAAG
  241 AGCATACAAT TCCACTGGAA AAACTCCAAC CAGATAAAGA TTCTGAGAAA TCAGGGCTCC
  301 TTCTTAACTA AAGGTCCATC CAAGCTGAAT GATCGCGCTG ACTCAAGAAG AAGCCTTTGG
  361 GACCAAGGAA ACTTTCCCCT GATCATCAAG AATCTTAAGA TAGAAGACTC AGATACTTAC
  421 ATCTGTGAAG TGGAGGACCA GAAGGAGGAG GTGCAATTGC TAGTGTTCGG ATTGACTGCC
  481 AACTCTGACA CCCACCTGCT TCAGGGGCAG AGCCTGACCC TGACCTTGGA GAGCCCCCCT
  541 GGTAGTAGCC CCTCAGTGCA ATGTAGGAGT CCAAGGGGTA AAAACATACA GGGGGGGAAG
  601 ACCCTCTCCG TGTCTCAGCT GGAGCTCCAG GATAGAGGCA CCTGGACATG CACTGTCTTG
  661 CAGAACCAGA AGAAGGTGGA GTTCAAAATA GACATCGTGG TGCTAGCTTT CCAGAAGGCC
  721 TCCAGCATAG TCTATAAGAA AGAGGGGGAA CAGGTGGAGT TCTCCTTCCC ACTCGCCTTT
  781 ACAGTTGAAA AGCTGACGGG CAGTGGCGAG CTGTGGTGGC AGGCGGAGAG GGCTTCCTCC
  841 TCCAAGTCTT GGATCACCTT TGACCTGAAG AACAAGGAAG TGTCTGTAAA ACGGGTTACC
  901 CAGGACCCTA AGCTCCAGAT GGGCAAGAAG CTCCCGCTCC ACCTCACCCT GCCCCAGGCC
  961 TTGCCTCAGT ATGCTGGCTC TGGAAACCTC ACCCTGGCCC TTGAAGCGAA AACAGGAAAG
 1021 TTGCATCAGG AAGTGAAACC TGGTGGTGAT GAGAGCCACT CAGCTCCAGA AAAATTTGGA
 1081 CCTGTGAGGT GTGGGGACCC CACCTTCCCT AGCCTGAATG CCTGAAGCTT GCAACCTGGG
1141 TAGAAACAAG GAGGCCAAAA GGTTCTTTTC
//
Primer: Reverse2
Sequence file: RDB12826_A4Gib.seq check
>01137_12826_A4Gi_Reverse2_B10_04.ab1
    1 AGGTAAATGA TTACGCCAGC GCGCAATTAA CCCTCACTAA AGGGAACAAA AGCTGGAGCT
   61 CCACCGCGGT GGCGGCCGCT CTAGAACTAG TGGATCCCCC GGGCTGCAGG AATTCGATGG
  121 CCTCGTGCCT CAAATGGGGC TACATGTCTT CTGAAACCGG TGAGGGCACT GGCAGGTCTT
  181 CTTCTCACTG AGGAGTCTCT TGATCTGAGA CATCCGCTCT GCTTGGCGCC TTCGGTGCCG
  241 GCACCTGACA CAGAAGAAGA TGCCTAGCCC AATGAAAAGC AGGAGGCCGG CGACGCCCCC
  301 CAGCACAATC AGGGCCATTG GCTGCACCGG GGTGGACCAT GTGGGCAGAA CCTTGATGTT
  361 GGATTCCAGC AGGACCTGTC CCGAGTCACT CAGCAGACAC TGCCACATCC CCGCCTCAGG
  421 GTTCAGCACC CACACCGCCT TCTCCCGCTT CGAGACCTTT GCCTCCTTGT TCTCCAGTTT
  481 CAAGCTCAGC ATCAGCTTAG GGGAGGTGGG TCCCCACACC TCACAGGTCA AATTTTTCTG
  541 GAGCTGAGTG GCTCTCATCA CCACCAGGTT CACTTCCTGA TGCAACTTTC CTGTTTTCGC
  601 TTCAAGGGCC AGGGTGAGGT TTCCAGAGCC AGCATACTGA GGCAAGGCCT GGGGCAGGGT
  661 GAGGTGGAGC GGGAGCTTCT TGCCCATCTG GAGCTTAGGG TCCTGGGTAA CCCGTTTTAC
  721 AGACACTTCC TTGTTCTTCA GGTCAAAGGT GATCCAAGAC TTGGAGGAGG AAGCCCTCTC
  781 CGCCTGCCAC CACAGCTCGC CACTGCCCGT CAGCTTTTCA ACTGTAAAGG CGAGTGGGAA
  841 GGAGAACTCC ACCTGTTCCC CCTCTTTCTT ATAGACTATG CTGGAGGCCT TCTGGAAAGC
  901 TAGCACCACG ATGTCTATTT TGAACTCCAC CTTCTTCTGG TTCTGCAAGA CAGTGCATGT
  961 CCAGGTGCCT CTATCCCTGG AGCTCCAGCT GAGACACGGG AGAGGGGTCT TTCCCCCCCC
 1021 CTGTATGTTT TTTACCCCTT TGCACTCCTA CATTTGCACT GAGGGGCTAC TACCAAGGGG
 1081 GCTCCTCCAA GGGTCAGGTT TCAGGCTCTG CCTCATGAGC CAAGTGGGTA TTCAGAAGCT
1141 GTGGGCAAGT TCCAAATC
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Tokito, S., Single amino acid substitution in the V3 domain of CD4 is responsible for OKT4 epitope deficiency. Immunogenetics, 34, 208-210 (1991). PMID 1716607. [link to RRC of NBRP]