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pAC201 (#RDB01177)

Human HLA - Cx52 (Cw12) genomic, p201-31

Alternative name Human HLA-Cx52 (Cw12) genomic clone (p201-31)
Clone info. Human HLA-Cx52 (Cw12) genomic DNA clone from AKIBA library; allele:HLA-Cw*1201
Vector backbone pUC19 (Plasmid)
Size of vector backbone 2.7 kb
Selectable markers Amp^r
Gene/insert name Human major histocompatibility complex, class I, C Genomic DNA
Reference of insert sequence D12471, D12472 (DDBJ accession number)
Depositor|Developer Takata, Hajime |
 
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB01177 pAC201 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pAC201 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB01177).

Reference section:

Ando, H., Miyata, Y., Takata, H., Inoko, H., Identification of alloantisera reacting with HLA-C blank (Cx52) using a mouse L-cell transfected with the HLA-Cw*1201 allele. Hum. Immunol., 36, 76-80 (1993). PMID 8463124.

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB13726_A5Llp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pUC_M13_-100
Sequence file: RDB13726_A5Lla.seq check
>01177_13726_A5Ll_pUC_M13_-100_C03_09.ab1
    1 GGCCAGCCAG GCGAAATAAG GTTGGGGTAC GCCAGGGTTT TCCCAGTCAC GACGTTGTAA
   61 AACGACGGGC CAGTGAATTC TAAAGCAGTA GAAGAGCCTG GCAGGGGGTT CTTTGCATCC
  121 GGTATTTAAT ACATTTTGTG TGACTGCCTT AAAACTAATG GCCTCCTTAT GATTTTTTTT
  181 AAAAAGGTGT TACAAAAATA TCACAAAAAT ATCAAGTGTC CAAATAAAAT ATGCACACTG
  241 CTTAGATGTG CATAGTTCAC GAAAACGGGC AGTGCTGGAG CGCTGGTGAA GAGCATTGGG
  301 ACTGCATGGA GCCCTCGCAA CTTTGAGGTG ATGACTACAG GCTCCCGGTT GCAATAGACG
  361 GTAACAAACC CTGCTTCTTT GTATTCAGGA GATGTTCTGG ACTCACACAA GGAAACTCGG
  421 GCTAGAGAAT GAGGATAATT TTAAATGCAA CAACCCAGAG TCACAGATCC ATAGTCTGCG
  481 AAAGTAAAAC AGGAGCTTTG AGAATTTAAT TGTAATGCAG TTTTGACACA GGTCTTTCAC
  541 AGATTGGAAT TCTAATCATT CAGGGATTAC CAATATTGTG CTACCTACTG TATCAATAAA
  601 CAAAAAGGAA ACTGGTCTCT ATGAGAATCT CTACCTGGTG CTTTCAGACA AAACTTCACC
  661 AGGTTTAAAG AGAAAACTCC TGACTCTACA CGTCCATTCC CAGGGCGAGC TCACTGTCTG
  721 GCATCAAGTT CCCCATGGTG AGTTTCCCTG TACAAGAGTC CAAGGGGAGA GGTAAGTGTC
  781 CTTTATTTTG CTGGATGTAG TTTAATATTA CCTGAGGTAA GGTAAGGCAA AGAGTGGGAG
  841 GCAGGGAGTC CAGTTCAGGG ACGGGGATTC CAGGAGAAGT GAAGGGGAAG GGGCTGGGCG
  901 CAGCCTGGGG GGTCTCTCCC TGGTTTCCAC AGACAGATCC TTGGCCAAGG ACTCAGGCAC
  961 ACAGTGTGAC AAAGATGCTT GGTGTAGGAA GAAGAGGGAT CAGGACGAAG TCCCAGGTCC
 1021 CGGGCCGGGG GCTCTCAGGT CTCAAGGCTT CAAGGTCCGT GTCTGCACTG GGGAAGCGCC
 1081 GCGTTTGAAG GAATCTCCAC TCCCCTTGAA GTTTCACTTC TTCTCCCAAA CCTGCGTCCG
1141 GGGTTCCTTC CTTCCTGGAA ATAAACTTCA AGTGAACCCG CGCGGTTCCC CCC
//
Primer: Reverse2
Sequence file: RDB13726_A5Llb.seq check
>01177_13726_A5Ll_Reverse2_B07_04.ab1
    1 ATGACCGGGG AATTGCGCCA AGCTTGCATG CCTGCAGGTC GACTCTAGAG GATCCCCGGG
   61 TACCGAGCTC GAATTCCGGA TCCCTTAGAA CTGGAGGTCC AGGGTTCAGT CCTTGCGCTG
  121 ATTCTTTTCC ACAGTCAATC ACTCCCTTGG TGCTTCATCC ATGCTTGAGG TTTTAAGTAT
  181 CGTTTATGTG GTGTGAGCTC CTAAATCTAT TTCTCCAGCC CAGTCCTTTC CCCTGAACTG
  241 TGGAGTTGTC TGCCCAGCTG CCACCCCAGC TCCCCCACCT GCATTCCTAG TAGACATCTC
  301 CTCCACTGAG TGCCTGTGAT GCCCCCTCCT CAGGATGCTC CTGCCAGAGT CTCCCCATCT
  361 CCACTGACAG CAGCTCCACC CTTCCTTCTA CTCACTCATT TTACAACTAT GGGTGTCCTT
  421 GATTCGTCTT TCTCACACCA CAGATACAAT CCATTGGCAA ATGCTGTGAG TCCATCTTCA
  481 AATGCATCCA GAATCCCCTC ACGTCCCACT ATTTCCTGTG CTCACACCCC AGACATCTCC
  541 AGCCTGGAAT ACTGCACTCG ATTCCTACTG TTTTCCCTTC TGCCTCCCTC ATCCCTCACC
  601 TCTCAATTCT GTTCTCAGCA CAGCCATCAG AGATCCTTTT AAGAAAGAAG TCATATCATG
  661 GCTCTCTTCT GCTCCAAACT GTCCTCTAAC TCCCCCATCC CACTCAGAGC AGAGGTCAGA
  721 CGCAACCCCA CTCCCCTCAA GCCCACCTGC TCTGGCCATA CCTCTGACCT CATCTAGTTT
  781 CTCTGTCCAG CCCTCCTGGC CTCCTTGCTC TTCTGGGAAC ACAGACACCT TCCTGCCCTA
  841 GTGCATTTGG ACTGGAGTTT CCTTGCCTAG AAAGAACTTC CCCCAGACAT CCTCATGTCC
  901 CTCAAATCTT TTCCTTCAAA GGGTCATCTT TGCAACAAGC CCACACATTG ACAAACTCCT
  961 GTCCAACAGC CACCTTCCCT GTCCCCCACT TGCCCATACC CGATCACCTG CCTCATGGCA
1021 CTTACAACCG TTCAATCACT TGTTTCTACT GTGATTATTA GTGCCA
//
Primer:
Sequence file: RDB13726_A5Llc.seq check
1

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Ando, H., Identification of alloantisera reacting with HLA-C blank (Cx52) using a mouse L-cell transfected with the HLA-Cw*1201 allele. Hum. Immunol., 36, 76-80 (1993). PMID 8463124.