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AM2.60 (#RDB01178)

Human HLA - B44 genomic DNA

Alternative name Human HLA-B44 genomic clone
Clone info. Human HLA-B44 genomic DNA clone from AM002 cell line library; allele:HLA-B*4401
Vector backbone pUC19 (Plasmid)
Size of vector backbone 2.7 kb
Selectable markers Amp^r
Gene/insert name Human major histocompatibility complex, class I, B Genomic DNA
Depositor|Developer Takata, Hajime |
 
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB01178 AM2.60 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The AM2.60 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB01178).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB13686_A5Jgp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: Amp_R
Sequence file: RDB13686_A5Jga.seq check
>01178_13686_A5Jg_Amp_R_B08_05.ab1
    1 TCGTTTCATA TTATTGAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA
   61 ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC
  121 TGACGTCTAA GAAACCATTA TTATCATGAC ATTAACCTAT AAAAATAGGC GTATCACGAG
  181 GCCCTTTCGT CTCGCGCGTT TCGGTGATGA CGGTGAAAAC CTCTGACACA TGCAGCTCCC
  241 GGAGACGGTC ACAGCTTGTC TGTAAGCGGA TGCCGGGAGC AGACAAGCCC GTCAGGGCGC
  301 GTCAGCGGGT GTTGGCGGGT GTCGGGGCTG GCTTAACTAT GCGGCATCAG AGCAGATTGT
  361 ACTGAGAGTG CACCATATGC GGTGTGAAAT ACCGCACAGA TGCGTAAGGA GAAAATACCG
  421 CATCAGGCGC CATTCGCCAT TCAGGCTGCG CAACTGTTGG GAAGGGCGAT CGGTGCGGGC
  481 CTCTTCGCTA TTACGCCAGC TGGCGAAAGG GGGATGTGCT GCAAGGCGAT TAAGTTGGGT
  541 AACGCCAGGG TTTTCCCAGT CACGACGTTG TAAAACGACG GCCAGTGAAT TCTAATCATT
  601 CAGGGATTAC CAATATTGTG CTACCTACTG TATTAATAAA CAAAAAGGAA ACTGGTCTCT
  661 ATGAGAATCT CTGTGTGGTG GCTTCAGACA AAACTTCGCC AGGTTTAGAG AGAAAACCCC
  721 TGTCTCTACA CCTCCATTCC CAGGGCGAGC TCACTCTCTG GCATCAAGTT CCCCGTGATC
  781 AGTTTCCCTA CACAGATCCA AGAGGAGAGG TAAGGAGTGA GAGGCAGGAG TCCAGTTCAG
  841 GACAGGATTC CAGGAGAGAG TGACGATGGG CTGGCGCACC TGGGGTCTCC TCCCTGGTTC
  901 CAACAGAACG ATCCTTGTCA GAACTCAGCC AGACAGTGTG ACCAAGAAGC TGGTGTAGGA
  961 AAGAGGAATC AGACGAATTC CAGGTTCTGG ACGGGCTCTC AGGACTCAAG CCTCGAGACC
1021 GTCTGAATGG AGGCCACGGT GGAATCCCAT CCACGAATTC CAGTATACGT TCG
//
Primer: M13_-40
Sequence file: RDB13686_A5Jgb.seq check
>01178_13686_A5Jg_M13(-40)_A06_03.ab1
    1 CATCCGGCAT CTATCATTCA GGGATTACCA ATATTGTGCT ACCTACTGTA TTAATAAACA
   61 AAAAGGAAAC TGGTCTCTAT GAGAATCTCT GTGTGGTGGC TTCAGACAAA ACTTCGCCAG
  121 GTTTAGAGAG AAAACCCCTG TCTCTACACC TCCATTCCCA GGGCGAGCTC ACTCTCTGGC
  181 ATCAAGTTCC CCGTGATCAG TTTCCCTACA CAAGATCCAA GAGGAGAGGT AAGGAGTGAG
  241 AGGCAGGGAG TCCAGTTCAG GGACAGGGAT TCCAGGAGGA GAAGTGAAGG GGAAGGGGCT
  301 GGGCGCAGCC TGGGGGTCTC TCCCTGGTTT CCACAGACAG ATCCTTGTCC AGGACTCAGG
  361 CAGACAGTGT GACAAAGAGG CTGGTGTAGG AGAAGAGGGA TCAGGACGAA GTCCCAGGTC
  421 CCGGACGGGG CTCTCAGGGT CTCAGGCTCC GAGGGCCGCG TCTGCAATGG GGAGGCGCAG
  481 CGTTGGGGAT TCCCCACTCC CACGAGTTTC ACTTCTTCTC CCAACCTATG TCGGGTCCTT
  541 CTTCCAGGAT ACTCGTGACG CGTCCCCATT TCCCACTCCC ATTGGGTGTC GGGTGTCTAG
  601 AGAAGCCAAT CAGTGTCGCC GGGGTCCCAG TTCTAAAGTC CCCACGCACC CACCCGGACT
  661 CAGAATCTCC TCAGACGCCG AGATGCGGGT CACGGCGCCC CGAACCCTCC TCCTGCTGCT
  721 CTGGGGGGCA GTGGCCCTGA CCGAGACCTG GGCCGGTGAG TGCGGGGTCG GGAGGGAAAT
  781 GGCCTCTGTG GGGAGGAGAG AGGGGACCGC AGGCGGGGGC GCAGGACCCG GGGAGCCGCG
  841 CCGGGAGGAG GGTCGGGCGG GTCTCAGCCC CTCCTCGCCC CCAGGCTCCC ACTCCATGAG
  901 GTATTTCTAC ACCGCCATGT CCCGGCCCGG CCGCGGGGAG CCCCGCTTCA TCACCGTGGG
  961 CTACGTGGAC GACACGCTGT TCGTGAGGTT CGACAGCGAC GCCCACGAGT CCGAGGAAGG
 1021 GAGCCGCGGG CGCCCATGGA TAGAAGCAAG GAAGGGGCCC GGGAGTATTG GGACCGGAGA
1081 ACACAGATCT CCGAGACCAC ACACAGGACT TACCGAAGAA GGAAACCC
//
Primer: Reverse2
Sequence file: RDB13686_A5Jgc.seq check
>01178_13686_A5Jg_Reverse2_B06_06.ab1
    1 AGTAATCATT ACGCCAGCTT GCATGCCTGC AGGTCGACTC TAGAGGATCC CCGGGTACCG
   61 AGCTCGAATT CTGTTCTTGG ATGTGTAGTT TCCAGCCTGG TTCTGTGGCC TTCCCCACAA
  121 TAAAACTAGC CCCCAATACC AATATACAAC TTTATGTGTA CATTACAGAA ATTTGGTTTC
  181 CATAGTTTTC TCCAAGAAGT GAGTGAGAAA TGAGTCTGTG GGCGAGTGTC AGAGAGCGGC
  241 ATTCAGAGGT GTTCTTTGTG CGAGAGCCAC ATCCTGAATT GTCTACCTGG CCTCTACCCC
  301 ATGGTGGAGA GAACAACAGA GAATATCACC TCTCATAACT GATGATATAC ATCCTCCCTT
  361 TTCTTTCTGT GAGAAAAATC CTCTTTTAAA CAGAGTTTGA AAACCCACCC CACCCACCCA
  421 CCCTGGGCAC TCTCTGATCA CTGATCTCAG TGGTTCCCAA TCTGTCTGAG CAATAGGATT
  481 GCTGGCGGGG ACTTAGAAAA TACACAGGCC ACTCCCCAGA ACCCTTGTCT CAGAGTATTA
  541 CGCACAAGAC CAAGGAATCA TTTATATGAC AAGCCCTAGA GGTGAGGCTG ATGCTCAGAC
  601 GTGTGGGATC CTGGTGTTCT TGCTACTCCA AGTGTGATCT GGAGACCAGC AACATGAGCT
  661 CCAGCCTTGT CATAAATCCA GAATCTCTTG CTCAACTCCA GACTTCCAGG ATCTCAGCAC
  721 CACATCCAGA TGATCCTGGT GCACATGGGG TTTCCTTGTC TGAGTGTCCT CTAGACGTGG
  781 GGCCAGAACT GTGCAGTCTG CTCTGGGTGT GGTCTGATCA CACCCCCTTA GAACTGGAGG
  841 TCCAGGGTTC AGTCCTTGTG CTCATTCTTT TCCATAGTCA GTCACTCCCT TTGTGCCTCA
  901 TCCATGCTTG AGGTTTTAAG TCTCATATAT ATGGTGTGAC CTCCTAAATC TATTTCTCCA
  961 GCCCAGTCCT TTCCCCTAAA CTCTGGGAGT TGTCTGTCCA AAATCCACCC AGCTCCCCCC
 1021 CACCCGCCTT TCCTAGTAGA CATCTCTCCA CTGAGTACTG TGATGCCCCC TCTCAGACGC
1081 TCTGCAGAGT CTCCCCATCT CAACTGACAG CAGCCTCCAA TTCCCAT
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

reference Zemmour, J., HLA class I nucleotide sequences, 1991. Tissue Antigens, 37, 174-180 (1991). PMID 1926127.