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pBluescript SKII+mLIF (#RDB01484)

Plasmid clone of mouse LIF (Leukemia inhibitory factor) cDNA

Alternative name Mouse LIF (leukemia inhibitory factor) cDNA
Clone info. Mouse LIF (leukemia inhibitory factor) cDNA
Vector backbone pBluescript II SK (+) (Plasmid)
Size of vector backbone 3.0 kb
Selectable markers Amp^r
Gene/insert name Mouse Leukemia inhibitory factor (LIF) cDNA
Depositor|Developer Hamada, Hirofumi |
 
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Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
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MTAに書く使用条件 非営利機関の非営利学術研究へ提供する。利用者が本件リソースを利用した研究成果に基づき特許申請、商業活動等を行う場合は、利用者は事前に寄託者に連絡する。利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する。利用者は、研究成果の公表にあたって謝辞の表明を必要とする。

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RDB01484 pBluescript SKII+mLIF DNA solution Please contact us.

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pBluescript SKII+mLIF was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB01484).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB19265_B1Itp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pUC-M13_-100 (Pr0310)
Region: T7 pro,insert 5'
Sequence file: RDB19265_B1Ita.seq check
>01484_19265_B1It_2_pUC-M13_-100_G01_07_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN ANGCCNGGGT TTTCCCNGTC NCGACGTTGT AAAACGACGG
   61 CCAGTGAGCG CGCGTAATAC GACTCACTAT AGGGCGAATT GGGTACCGGG CCCCCCCTCG
  121 AGGTCGACGG TATCGATAAG CTTGATATCG AATTCTAGAC ACCATGAAGG TCTTGGCCGC
  181 AGGGATTGTG CCCTTACTGC TGCTGGTTCT GCACTGGAAA CACGGGGCAG GGAGCCCTCT
  241 TCCCATCACC CCTGTAAATG CCACCTGTGC CATACGCCAC CCATGCCACG GCAACCTCAT
  301 GAACCAGATC AAGAATCAAC TGGCACAGCT CAATGGCAGC GCCAATGCTC TCTTCATTTC
  361 CTATTACACA GCTCAAGGGG AGCCGTTTCC CAACAACGTG GAAAAGCTAT GTGCGCCTAA
  421 CATGACAGAC TTCCCATCTT TCCATGGCAA CGGGACAGAG AAGACCAAGT TGGTGGAGCT
  481 GTATCGGATG GTCGCATACC TGAGCGCCTC CCTGACCAAT ATCACCCGGG ACCAGAAGGT
  541 CCTGAACCCC ACTGCCGTGA GCCTCCAGGT CAAGCTCAAT GCTACTATAG ACGTCATGAN
  601 GGGCCTCCTC AGCAATGTGC TTTGCCGTCT GTGCNACAAG TANCGTGTGG GCNACGNNNA
  661 NGTGCNANNN GTCCCNNANN ACTCNNNNNA ANANNCNNTN NNANNNNNAA NNNTNNGNNT
721 NNNNNNNCNT NNNNGGGNNN NNNNNNN
//
Primer: reverse-2 (Pr0100)
Region: T3 pro,insert 3'
Sequence file: RDB19265_B1Itb.seq check
>01484_19265_B1It_2_reverse2_H01_08_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN CTCNNNNNNN NGGAACAAAA GCTGGAGCTC
   61 CACCGCGGTG GCGGCCGCTC TAGAACTAGT GGATCCTAGA AGGCCTGGAC CACCACACTT
  121 ATGACTTGCT TGTATGTCCC CAGAAGCTGG CAACCCAACT TTTTCCTTTG GAAGGCTTCT
  181 TTGTCAGAGT GGTCGGGGAC AGGTGGCACA TCCACGTGGC CCACACGGTA CTTGTTGCAC
  241 AGACGGCAAA GCACATTGCT GAGGAGGCCC CTCATGACGT CTATAGTAGC ATTGAGCTTG
  301 ACCTGGAGGC TCACGGCAGT GGGGTTCAGG ACCTTCTGGT CCCGGGTGAT ATTGGTCAGG
  361 GAGGCGCTCA GGTATGCGAC CATCCGATAC AGCTCCACCA ACTTGGTCTT CTCTGTCCCG
  421 TTGCCATGGA AAGATGGGAA GTCTGTCATG TTAGGCGCAC ATAGCTTTTC CACGTTGTTG
  481 GGAAACGGCT CCCCTTGAGC TGTGTAATAG GAAATGAAGA GAGCATTGGC GCTGCCATTG
  541 AGCTGTGCCA GTTGATTCTT GATCTGGTTC ATGAGGTTGC CGTGGCATGG GTGGCGTATG
  601 GCACAGGTGG CATTTACAGG GGTGNNNNNN NAGANGGCTC CCNGCCCCGT GTTTCCNGTG
  661 CANNNNNNGC AGCNNNNNNN GCNNNNTCCC TGCGGNNNNN ANNTTNATGN NGNCNANNNN
721 TNNNNNNNNN N
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

user_report Nakamura H., Alteration of the timing of implantation by in vivo gene transfer: delay of implantation by suppression of nuclear factor kappaB activity and partial rescue by leukemia inhibitory factor. Biochem. Biophys. Res. Commun., 321, 886-892 (2004). PMID 15358109. [link to RRC of NBRP]