Please review the QC test results indicated by icon below as well as clone information before placing your order.

pCAhMIF

Expression vector of human macrophage migration inhibitory factor

Remarks, protocol and/or map (gif)	RDB01526.gif

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution

The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR and an acknowledgment to the DEPOSITOR are requested. Additional terms and conditions: Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.

Ordering

Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

提供案内 (日本国内) [open/close]

提供条件	非営利機関の非営利学術研究へ提供する。利用者が本件リソースを利用した研究成果に基づき特許申請、商業活動等を行う場合は、利用者は事前に寄託者に連絡する。利用者は、研究成果の公表にあたって寄託者の指定する文献を引用し、研究成果の公表にあたって謝辞の表明を必要とする。 付加的提供条件： CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB15998_A8Gop1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pCAG-F Sequence file: RDB15998_A8Goa.seq
>01526_15998_A8Go_1_pCAG-F_A01_01_ABI24.ab1     1 GGGGGTTTTC CGGGGCTTTC TGGCGTGTGA CCGGCGGCTC TACTATCCTC TGCTAACCAT    61 GTTCATGCCT TCTTCTTTTT CCTACAGCTC CTGGGCAACG TGCTGGTTAT TGTGCTGTCT   121 CATCATTTTG GCAAAGAATT CTAGACCATC ATGCCGATGT TCATCGTAAA CACCAACGTG   181 CCCCGCGCCT CCGTGCCGGA CGGGTTCCTC TCCGAGCTCA CCCAGCAGCT GGCGCAGGCC   241 ACCGGCAAGC CCCCCCAGTA CATCGCGGTG CACGTGGTCC CGGACCAGCT CATGGCCTTC   301 GGCGGCTCCA GCGAGCCGTG CGCGCTCTGC AGCCTGCACA GCATCGGCAA GATCGGCGGC   361 GCGCAGAACC GCTCCTACAG CAAGCTGCTG TGCGGCCTGC TGGCCGAGCG CCTGCGCATC   421 AGCCCGGACA GGGTCTACAT CAACTATTAC GACATGAACG CGGCCAATGT GGGCTGGAAC   481 AACTCCACCT TCGCCTAAGG ATCTTTTTCC CTCTGCCAAA AATTATGGGG ACATCATGAA   541 GCCCCTTGAG CATCTGACTT CTGGCTAATA AAGGAAATTT ATTTTCATTG CAATAGTGTG   601 TTGGAATTTT TTGTGTCTCT CACTCGGAAG GACATATGGG AGGGCAAATC ATTTAAAACA   661 TCAGAATGAG TATTTGGTTT AGAGTTTGGC AACATATGCC CATATGCTGG CTGCCATGAA   721 CAAAGGTTTG GCTATAAAGA GGTCATCAGT ATATGAAACA GCCCCCTGCT GTCCATTCCG   781 TTATTCCATA GAAAAGCCTT GACTTGAGGT TTAGATTTTT TTTTTATATT TTGTTTTGTG   841 TTATTTTTGT TTCGTTTTAA CATCCCCTAA AAAATTTTTC CCTTTACATG GTTTTTTACT 901 AAGCCAGAAA TTTTTTCCAT CCCTGCTCCC TGAACTTACG TCCT //
Primer: pCA-bgter Sequence file: RDB15998_A8Gob.seq
>01526_15998_A8Go_1_pCA-bgter_B01_04_ABI24.ab1     1 AGCCCGGAAG TCCATAATTT TTGGCAGAGG GAAAAAGATC CTTAGGCGAA GGTGGAGTTG    61 TTCCAGCCCA CATTGGCCGC GTTCATGTCG TAATAGTTGA TGTAGACCCT GTCCGGGCTG   121 ATGCGCAGGC GCTCGGCCAG CAGGCCGCAC AGCAGCTTGC TGTAGGAGCG GTTCTGCGCG   181 CCGCCGATCT TGCCGATGCT GTGCAGGCTG CAGAGCGCGC ACGGCTCGCT GGAGCCGCCG   241 AAGGCCATGA GCTGGTCCGG GACCACGTGC ACCGCGATGT ACTGGGGGGG CTTGCCGGTG   301 GCCTGCGCCA GCTGCTGGGT GAGCTCGGAG AGGAACCCGT CCGGCACGGA GGCGCGGGGC   361 ACGTTGGTGT TTACGATGAA CATCGGCATG ATGGTCTAGA ATTCTTTGCC AAAATGATGA   421 GACAGCACAA TAACCAGCAC GTTGCCCAGG AGCTGTAGGA AAAAGAAGAA GGCATGAACA   481 TGGTTAGCAG AGGCTCTAGA GCCGCCGGTC ACACGCCAGA AGCCGAACCC CGCCCTGCCC   541 CGTCCCCCCC GAAGGCAGCC GTCCCCCCGC GGACAGCCCC GAGGCTGGAG AGGGAGAAGG   601 GGACGGCGGC GCGGCGACGC ACGAAGGCCC TCCCCGCCCA TTTCCTTCCT GCCGGCGCCG   661 CACCGCTTCG CCCCGCGCCC GCTAGAGGGG GTGCGGCGGC GCCTCCCAGA TTTCGGCTCC   721 GCACAGATTT GGGACAAAGG AAGTCCCTGC GCCCTCTCGC ACGATTACCA TAAAAGGCAA   781 TGGCTGCGGC TCGCCGCGCC TCGACAGCCG CCGGCGCTCC GGGGGGCCGC CGCGCCCCCT   841 CCCCCGAGCC CTCCCCGGCC CGAGGCGGCC CCGCCCCCGC CCGGGCACCC CCCACCCTGC   901 CGCCACCCCC CCGCCCGGGC ACGGCGAGCC CCGCGCCCAC GCCCCCGTAC GGGAGCCCCG   961 CACCCGAAGC CCGGGCCCGT GCTCAGCAAC TCTGGAGGGG GGTGCAGCAG TGGGGGTGCC 1021 GGGCGGAGCG GGGCCGCCTT CGGGCCGAGA GGCTCGGGGG AGGGGCGTCG G //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.10.12

GNP_filter3_RDBDEP_html_221010.pl