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pCAmB7

Expression vector of mouse B7 (CD80) cDNA

Catalog number RDB01530
Resource name pCAmB7
Alternative name Mouse B7 (CD80)
Clone info. Expression vector of mouse B7 (CD80) gene in mammalian cells
Vector backbone pCAGGS (Plasmid)
Size of vector backbone 4.8 kb
Selectable markers Amp^r (E. coli)
Gene/insert name Mouse CD80 (B7-1) cDNA
Depositor|Developer Hamada, Hirofumi |
Other clones in our bank
External Database
Mouse CD80 (B7-1)
          Reference sequence
            
           
          Remarks, protocol and/or map (gif) RDB01530.gif

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.(Cell, 33, 135-143 (1983); J. Virol., 45, 555-564 (1983).) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
          Additional terms and conditions:
          Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          Please visit Information of Request for Distribution.[link] 
          提供案内 (日本国内) [open/close]

          提供条件 非営利機関の非営利学術研究へ提供する。利用者が本件リソースを利用した研究成果に基づき特許申請、商業活動等を行う場合は、利用者は事前に寄託者に連絡する。利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Cell, 33, 135-143 (1983); J. Virol., 45, 555-564 (1983).)。利用者は、研究成果の公表にあたって謝辞の表明を必要とする。
          付加的提供条件：
          CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。
          提供依頼
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          手続きの概要は、「提供申込みについて[link]」をご覧ください。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB01530 pCAmB7 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The pCAmB7 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB01530).

          Reference section:

          Further references such as user reports and related articles (go to bottom)

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          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB18580_B0J1p1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CAG_R1 (Pr0584)
          Region: CAG pro,insert 5'
          Sequence file: RDB18580_B0J1a.seq check
          >01530_18580_B0J1_1_CAG_R1_2.5_A12_03_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NTCNNNCGGG CGCGGGGCGA ANCGGTGCGG CGCCGGCAGG
             61 AAGGAAATGG GCGGGGAGGG CCTTCGTGCG TCGCCGCGCC GCCGTCCCCT TCTCCCTCTC
            121 CAGCCTCGGG GCTGTCCGCG GGGGGACGGC TGCCTTCGGG GGGGACGGGG CAGGGCGGGG
            181 TTCGGCTTCT GGCGTGTGAC CGGCGGCTCT AGAGCCTCTG CTAACCATGT TCATGCCTTC
            241 TTCTTTTTCC TACAGCTCCT GGGCAACGTG CTGGTTATTG TGCTGTCTCA TCATTTTGGC
            301 AAAGAATTAG ACGGCATCGC AGCTTGGATA CACGCCGCCC ACGTGAAGGC TGCCGACCCC
            361 GGGGGTGGAC CATCCTCTAG ACTGCCATGG CTTGCAATTG TCAGTTGATG CAGGATACAC
            421 CACTCCTCAA GTTTCCATGT CCAAGGCTCA TTCTTCTCTT TGTGCTGCTG ATTCGTCTTT
            481 CACAAGTGTC TTCAGATGTT GATGAACAAC TGTCCAAGTC AGTGAAAGAT AAGGTATTGC
            541 TGCCTTGCCG TTACAACTCT CCTCATGAAN ANGAGTCTGA AGANCGAATC TACTGNCAAA
            601 AACATGACAA AGTGGTGCTG TCTGTCATTG CTGGGAAACT AAAAGTGTGG NCCGANTATN
            661 AGAACCGGAN TTTATATGAN NACACTACCT ACTCTCTTAT CNTCNTNGGN CNNNNNNNTT
          721 NNNANNNNGG NNNNNNNNNN
          //
          Primer: pCAGGS_R1 (Pr0614)
          Region: insert 3'
          Sequence file: RDB18580_B0J1b.seq check
          >01530_18580_B0J1_1_pCAGGS_R1_B02_02_ABI08.ab1
              1 NNNNNNNNNN NNNNNCNATT GNATGNAAAN AAATTTCCTT TATTAGCCNG AAGTCAGATG
             61 CTCAAGGGGC TTCATGATGT CCCCATAATT TTTGGCAGAG GGAAAAAGAT CTCAGTGGTA
            121 TTTGTGAGCC AGGGCATTGG CCACACCAGC CACCACCTTC TGATAGGCAG CCTGCACCTG
            181 AGGAGTGAAT TATCCTGTCT TTAACAAATT GGACTAATCC GGATCTAAAG GAAGACGGTC
            241 TGTTCAGCTA ATGCTTCTTC AGGCCCGAAG GTAAGGCTGT TGTTTGTTTC TCTGCTTGCC
            301 TCATTTCTTC TGAAACAGCT TCTGTGCTTA CAGAAGCATT TGATGATGAC AACGATGACG
            361 ACGACTGTTA TTACTGCGCC GAATCCTGCC CCAAAGAGCA CAAGTGTGTT CTTGCTATCA
            421 GGAGGGTCTT CTGGGGGTTT TTCCCAGGTG AAGTCCTCTG ACACGTGAGC ATCTCCATAT
            481 TTAATGAGAC ACTTAATGGT GTGGTTGCGA GTCGTATTGA AATCTAGTTG GCTACTAATG
            541 GTGTACAATT CAGATTCAGG ATCCTGGGAA ATTGTCGTAT TGATGCCAGG TAATTCTCTT
            601 CCATTTTCCA ACCAAGAGAA GCGAGGCTTT GGGAAACCCC CGGAAGCAAA GCAGGTAATC
            661 CTTTTAGTGT CTGCAGATGG GTTTCCAGAC TCAGTTATGT TGGGGGTAGA GAAGTCAGCT
            721 TTGATGGACA ACTTTACTAA AGCCAAGTGT TTAACTTCAT ACGTTCCTCT TTCCTTCTTT
            781 TGAACGACAC AGCTGTATGT GCCCCGGTCT GAAAGGACCA GGCCCAGGNT GANAAGAGAG
            841 TANGTAGTGT TGTCATATAA AGTCCGGTTC TTATACTCNG GCCACACTTT TANTTNCCCA
            901 GCAATGACAG ACAGCACCAC TTNNNCATGT TTTTGCNNGT ANANTCNGNC TTCNNNNTCA
          961 TCTTNNTNNN GNNNNNNGNN NNNGNNNNGN NNNNNNNNN
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          2023.08.18

          GNP_filter3_RDBDEP_html_230702.pl