Please review the QC test results indicated by icon below as well as clone information before placing your order.

pHM31

Human mu-calpain large subunit cDNA

Sequence (full)	RDB01652zzt01.seq compiled by us
Remarks, protocol and/or map (gif)	RDB01652.gif

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Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

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Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB01652_B2D7p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pUC-CAPbs_up_F (Pr0536) Region: insert 5'(5' UTR,CDS) Sequence file: RDB01652_B2D7a.seq
>01652_01652_B2D7_2_pUC-CAPbs_up_F_B01_02_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNGNNNNNN NNCAATTAAT GTGAGTTAGC TCACTCATTA    61 GGCACCCCAG GCTTTACACT TTATGCTTCC GGCTCGTATG TTGTGTGGAA TTGTGAGCGG   121 ATAACAATTT CACACAGGAA ACAGCTATGA CCATGATTAC GCCAAGCTTG CATGCCTGCA   181 GGTCGACTCT AGAGGATCCC CGGGTACCGA GCTCGAATTC CGGGCCTTTT ATTATTTGGG   241 GTTCCTCTAT ATGGAACACG GGACCTCCCC ACCCCAAGCC TAGTGCCTCT GGCACCCCCA   301 TAACCAGTAC ACAAGTCCCT GAAGAGTCCC CTTTAAAATC ACTAATATAA AAAAGGAGCG   361 AAGGAGGGAA GGCCGACCGC CCACAGGCTG GGCAGCTCCT GGTCGGCAGC AGGGCCAGGG   421 AAGGACAGAG GGGGCGTTGG CTTCGGCGCA GGAAGGCACA GGCGTTCCCA GCCCCGGGGC   481 CTCCCCGAGC CGGGAGGCGG CTCCACACGC CTGGACTGCA GACTGTGTCG AGCTAGTGGT   541 TATAGTTTAT AGTCTGCAGG CAAGGCCAGG CCGGTGGTGC TGAGTGGGTG GCCTCTGAAG   601 TGGGGAGAGG GGACACATCA GGATGCTGGG GAAACCCGGG GCTGTCCCGA GGCAGGAACA   661 AGCGAAAGCT GCCTTCCTGG CTTGGCAATG GGTGGGGCCC AGGGAGTCCA TCCCGAGCCC   721 ATGGCTGGCA CAGGCAGGGG CCACACAGTT CTGCCCGGAG CAGATGAACG ATGGTGGCTG   781 GGANGAGAGG GGACAGGANG ANGANGCTGC CTCTCTGCTC CNAGGAANGC ACTTGCAGCT   841 GGGGTGGNCT GNTGTGGTGT GGTANGAGGN GANGCTTGNC ANANNANGAN GGANGGGANC   901 ACGGNAANGG GGANCNANTC CCTGNNTCAT GNNAACATGG NCAGCTGCAA CCACTTAAAC   961 NAGNCNAAGG TCNNNACTCN NTCCNNATCT GTGTNNNNAN TTTTGAAAAA TCGNNNNNNN 1021 GNNCNCNANN CNNCNNNNNN NNNCAAACNA ANNNNNNNAN NNNNNNNNN //
Primer: pUC-M13_-100 (Pr0310) Region: insert 3' (3' UTR) Sequence file: RDB01652_B2D7b.seq
>01652_01652_B2D7_2_pUC-M13_-100_B03_06_ABI24.ab1     1 NNNNNNNNNN NNGNANGCNN GGGTTTTCCN NGTCNCGACG TTGTAAAACG ACGGCCAGTG    61 AATTCCGGGG GGGGGAAGGA GAGAGGGAGG GCGGAGGGCG GAGGGGCGGC GGGAGGAGGG   121 CGGGGAGGAG CGCTCTTCCT GGTTGGGCCC TGCCCTGAGC TGCCACCGGG AAGCCAGCCT   181 CAGGGACTGC AGCGACCCCC AAACACCCCT CCCCCAGGAT GTCGGAGGAG ATCATCACGC   241 CGGTGTACTG CACTGGGGTG TCAGCCCAAG TGCAGAAGCA GCGGGCCAGG GAGCTGGGCC   301 TGGGCCGCCA TGAGAATGCC ATCAAGTACC TGGGCCAGGA TTATGAGCAG CTGCGGGTGC   361 GATGCCTGCA NAGTGNGACC NTCNTCCGNG ANGANNNCTT NCCCCCCGNN NNNNNNNANN 421 NNNNGTTACN NNGNNNNGGN NNNNNNNNN //
Primer: HM31-B_R (Pr1193) Region: insert mid(3' UTR,CDS) Sequence file: RDB01652_B2D7c.seq
>01652_01652_B2D7_2_HM31-B_R_B02_02_ABI08.ab1     1 NNNNNNNNNN NNNNNNNGNN GNGNNGCTTG GCAGANGAGG AGGGAGGGGA GCACGGCAAG    61 GGGGACCGAG TCCCTGCCTC ATGCAAACAT GGTCAGCTGC AACCACTTAA ACAAGTCAAA   121 GGTCACAACT CCATCCAGAT CTGTGTCCAG AGTTTTGAAA AATCGGAACA TGGTCTCTAG   181 CCGCACCAGG CAGCAAACGA AATTGTCAAA GTCGACCGCC AGGTCGGGCT CCGAGTAGCG   241 GGTGATGATG AGCTCGTACA GCTTCTTGTT GAGCTTGAAG CCTGCCGACT CAATGGCCAT   301 CCGCATCTCG TAGGCACTCA TGCTGCCCGA CTTGTCCAGG TCAAACTTCC GGAAGATGGA   361 CAGGTAATTC CGGATGCGGT TCCACAGGAT GTTGAACTCC ACCAGGCCCA GCTTCCCATT   421 GCCATCACGA TCCATGAGGT TCACCATGCT GCGGCACGAC TCTAGGCTGA AGCCCTTGGT   481 CCGCAGGTCT TTGTGTTTGC TGATGATCCT ATTGAGGATT GTCCGCAACT CCTTCACGCT   541 GATCTCCATG TCCTCCCCTG CCAGCTGCCT GAAGAGGGCC TTGAAGTTCT CGTCAATCTC   601 CTCTTCTGAG AGCACTTGCT CATCGGGGAN ANTGGCCTGN ANCTGGTCNT CCNGCTCCNC   661 AGTCCCNGCA CTCTTCTCNG ANAAGAANCN CANCACGAAN TCNCCCNNNN NNNNNGGNTC 721 NNNNNNNNNG NNNNNNNNCN TNNNNCNNNN NNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.01

GNP_filter3_RDBDEP_html_230501.pl