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pCALwL (#RDB01679)

A cassette plasmid to insert cDNA into downstream CAG promoter

Clone info. A cassette plasmid to insert cDNA into downstream CAG promoter under ON-expression condition, which can be switched OFF (i.e., removed) by the Cre-expressing adenovirus.
Vector backbone pCALwL (Plasmid)
Size of vector backbone 4,931 bp
Selectable markers Amp^r (E. coli)
Gene/insert name P1 phage loxP
Depositor|Developer Saito, Izumu |
 
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Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Kanegae, Y., Nucleic Acids Res., 23, 3816-3821(1995)).
Additional terms and conditions:
Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Kanegae, Y., Nucleic Acids Res., 23, 3816-3821(1995))。
MTAに書く付加的使用条件：
CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB01679 pCALwL DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCALwL was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB01679).

Reference section:

Kanegae, Y., Lee, G., Sato, Y., Tanaka, M., Nakai, M., Sakaki, T., Sugano, S., Saito, I., Efficient gene activation in mammalian cells by using recombinant adenovirus expressing site-specific Cre recombinase. Nucleic Acids Res., 23, 3816-3821 (1995). PMID 7479022. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB16804_A8Lpp2-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pCAGGS_R1 ()
Sequence file: RDB16804_A8Lpa.seq check
>01679_16804_A8Lp_3_pCAGGS_R1_G02_20_ABI24.ab1
    1 CGGCAAAAAA TTCCACACAC TATTGCAATG AAAATAAATT TCCTTTATTA GCCAGAAGTC
   61 AGATGCTCAA GGGGCTTCAT GATGTCCCCA TAATTTTTGG CAGAGGGAAA AAGATCTCAG
  121 TGGTATTTGT GAGCCAGGGC ATTGGCCACA CCAGCCACCA CCTTCTGATA GGCAGCCTGC
  181 ACCTGAGGAG TGAATTGATT TGATCCCCGG GTACCGAGCG ACTCGAGATA ACTTCGTATA
  241 ATGTATGCTA TACGAAGTTA TACGCGTTCG GATTTAAATC CGACTCGAGA TAACTTCGTA
  301 TAATGTATGC TATACGAAGT TATACGCGTT CGCTCGAATT AATCAATTCT TTGCCAAAAT
  361 GATGAGACAG CACAATAACC AGCACGTTGC CCAGGAGCTG TAGGAAAAAG AAGAAGGCAT
  421 GAACATGGTT AGCAGAGGCT CTAGAGCCGC CGGTCACACG CCAGAAGCCG AACCCCGCCC
  481 TGCCCCGTCC CCCCCGAAGG CAGCCGTCCC CCCGCGGACA GCCCCGAGGC TGGAGAGGGA
  541 GAAGGGGACG GCGGCGCGGC GACGCACGAA GGCCCTCCCC GCCCATTTCC TTCCTGCCGG
  601 CGCCGCACCG CTTCGCCCCG CGCCCGCTAG AGGGGGTGCG GCGGCGCCTC CCAGATTTCG
  661 GCTCCGCACA GATTTGGGAC AAAGGAAGTC CCTGCGCCCT CTCGCACGAT TACCATAAAA
  721 GGCAATGGCT GCGGCTCGCC GCGCCTCGAC AGCCGCCGGC GCTCCGGGGG CCGCCGCGCC
781 CCTCCCCCGA GCC
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Kanegae, Y., Efficient gene activation in mammalian cells by using recombinant adenovirus expressing site-specific Cre recombinase. Nucleic Acids Res., 23, 3816-3821 (1995). PMID 7479022. [link to RRC of NBRP]
reference Niwa, H., Efficient selection for high-expression transfectants with a novel eukaryotic vector. Gene, 108 (2): 193-200 (1991). PMID 1660837. [link to RRC of NBRP]
user_report Mitsui, S., A mental retardation gene, motopsin/prss12, modulates cell morphology by interaction with seizure-related gene 6. Biochem. Biophys. Res. Commun., 436 (4): 638-644 (2013). PMID 23770366. [link to RRC of NBRP]
user_report Maeda M., Targeted gene therapy toward astrocytoma using a Cre/loxP-based adenovirus system. Brain Res., 1081, 34-43 (2006). PMID 16529724. [link to RRC of NBRP]
user_report Kamei, K., Transgenic mouse for conditional, tissue-specific Cox-2 overexpression Genesis, 44, 177-182 (2006). PMID 16604526. [link to RRC of NBRP]
user_report Kudo T., Transgenic expression of a dominant-negative connexin26 causes degeneration of the organ of Corti and non-syndromic deafness. Hum. Mol. Genet., 12, 995-1004 (2003). PMID 12700168. [link to RRC of NBRP]
user_report Honma M., Developmental alteration of nerve injury induced glial cell line-derived neurotrophic factor (GDNF) receptor expression is crucial for the determination of injured motoneuron fate. J. Neurochem., 82, 961-975 (2002). PMID 12358802. [link to RRC of NBRP]
user_report Nagayama Y., Targeting the replication of adenovirus to p53-defective thyroid carcinoma with a p53-regulated Cre/loxP system. Cancer Gene Ther., 8, 36-44 (2001). PMID 11219492. [link to RRC of NBRP]