RDB01692 - pCM-TK

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

pCM-TK (#RDB01692)

CM-HSV-TK genomic DNA

Alternative name	CM-TK
Clone info.	HSV TK (thymidine kinase) under regulation of CMV promoter.
Comment	CMV promoter was from pUHD 15-1. PhCMV*-1 and SV40 poly(A) sequences have been removed from pUHD 10-3 vector.
Vector backbone	pUHD10-3 (Plasmid)
Size of vector backbone	6,646 bp
Selectable markers	Amp^r
Gene/insert name	HSV thymidine kinase Genomic DNA
Depositor\|Developer	Hamada, Hirofumi \|

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Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature (J. Leukoc. Biol., 60, 181-190 (1996)) designated by the DEPOSITOR and an acknowledgment to the DEPOSITOR are requested.

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature (J. Leukoc. Biol., 60, 181-190 (1996)) designated by the DEPOSITOR and an acknowledgment to the DEPOSITOR are requested.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	非営利機関の非営利学術研究へ提供する。利用者が本件リソースを利用した研究成果に基づき特許申請、商業活動等を行う場合は、利用者は事前に寄託者に連絡する。利用者は、研究成果の公表にあたって寄託者の指定する文献 (J. Leukoc. Biol., 60, 181-190 (1996)) を引用し、謝辞の表明を必要とする。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB01692	pCM-TK	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCM-TK was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB01692).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB15400_A7Iep1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward Sequence file: RDB15400_A7Iea.seq
>01692_15400_A7Ie_CMV_Forward_A01_01_ABI08.ab1 1 ACGTATCACT CCGCCCATTG ACGCAAATGG GCGGTAGGCG TGTACGGTGG GAGGTCTATA 61 TAAGCAGAGC TCGTTTAGTG AACCGTCAGA TCGCCTGGAG ACGCCATCCA CGCTGTTTTG 121 ACCTCCATAG AAGACACCGG GACCGATCCA GCCTCCGCGG CCCCGAATTC CGGGGATCGA 181 TCTTGGTGGC GTGAAACTCC CGCACCTCTT CGGCAAGCGC CTTGTAGAAG CGCGTATGGC 241 TTCGTACCCC TGCCATCAAC ACGCGTCTGC GTTCGACCAG GCTGCGCGTT CTCGCGGCCA 301 TAGCAACCGA CGTACGGCGT TGCGCCCTCG CCGGCAGCAA GAAGCCACGG AAGTCCGCCT 361 GGAGCAGAAA ATGCCCACGC TACTGCGGGT TTATATAGAC GGTCCTCACG GGATGGGGAA 421 AACCACCACC ACGCAACTGC TGGTGGCCCT GGGTTCGCGC GACGATATCG TCTACGTACC 481 CGAGCCGATG ACTTACTGGC AGGTGCTGGG GGCTTCCGAG ACAATCGCGA ACATCTACAC 541 CACACAACAC CGCCTCGACC AGGGTGAGAT ATCGGCCGGG GACGCGGCGG TGGTAATGAC 601 AAGCGCCCAG ATAACAATGG GCATGCCTTA TGCCGTGACC GACGCCGTTC TGGCTCCTCA 661 TATCGGGGGG GAGGCTGGGA GCTCACATGC CCCGCCCCCG GCCCTCACCC TCATCTTCGA 721 CCGCCATCCC ATCGCCGCCC TCCTGTGCTA CCCGGCCGCG CGATACCTTA TGGGCAGCAT 781 GACCCCCCAG GCCGTGCTGG CGTTCGTGGC CCTCATCCCG CCGACCTTGC CCGGCACAAA 841 CATCGTGTTG GGGGCCCTTC CGGAGGACAG ACACATCGAC CGCCTGGCCA AACGCCAGCG 901 CCCCGGCGAG CGGCTTGACC TGGCTATGCT GGCCGCGATT CGCCGCGTTT ACGGGCTGCT 961 TGCCAATACG GTGCGGTATC TGCAGGGCGG CGGGTCGTGG CGGGAGGATT GGGGACAGCT 1021 TTCGGGACGG CCGTGCCGCC CCAGGGTGCC GAGCCCCAGA GCAACGCCGG CTCACGACCC 1081 CATATTCGGG GAACACGTTA ATTAACCTTG AATCCGGGCC CCGAAGTTGC TGGGTCCCCC 1141 CCAAACG //
Primer: HSV TK pA_R Sequence file: RDB15400_A7Ieb.seq
>01692_15400_A7Ie_HSV_TK_pA_R_C11_08_ABI24.ab1 1 AGGGGACTTC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCATC TCCCGGGCAA 61 ACGTGCGCGC CAGGTCGCAG ATCGTCGGTA TGGAGCCGGG GGTGGTGACG TGGGTCTGGA 121 CCATCCCGGA GGTAAGTTGC AGCAGGGCGT CCCGGCAGCC GGCGGGCGAT TGGTCGTAAT 181 CCAGGATAAA GACGTGCATG GGACGGAGGC GTTTGGCCAA GACGTCCAAG GCCCAGGCAA 241 ACACGTTGTA CAGGTCGCCG TTGGGGGCCA GCAACTCGGG GGCCCGAAAC AGGGTAAATA 301 ACGTGTCCCC GATATGGGGT CGTGGGCCCG CGTTGCTCTG GGGCTCGGCA CCCTGGGGCG 361 GCACGGCCGT CCCCGAAAGC TGTCCCCAAT CCTCCCGCCA CGACCCGCCG CCCTGCAGAT 421 ACCGCACCGT ATTGGCAAGC AGCCCGTAAA CGCGGCGAAT CGCGGCCAGC ATAGCCAGGT 481 CAAGCCGCTC GCCGGGGCGC TGGCGTTTGG CCAGGCGGTC GATGTGTCTG TCCTCCGGAA 541 GGGCCCCCAA CACGATGTTT GTGCCGGGCA AGGTCGGCGG GATGAGGGCC ACGAACGCCA 601 GCACGGCCTG GGGGGTCATG CTGCCCATAA GGTATCGCGC GGCCGGGTAG CACAGGAGGG 661 CGGCGATGGG ATGGCGGTCG AAGATGAGGG TGAGGGCCGG GGGCGGGGCA TGTGAGCTCC 721 CAGCCTCCCC CCCGATATGA GGAGCCAGAA CGGCGTCGGT CACGGCATAA GGCATGCCCA 781 TTGTTATCTG GGCGCTTGTC ATTACCACCG CCGCGTCCCC GGCCGATATC TCACCCTGGT 841 CGAGGCGGTG TTGTGTGGTG TAGATGTTCG CGATTGTCTC GGAAGCCCCC AGCACCTGCC 901 AGTAAGTCAT CGGCTCGGGT ACGTAGACGA TATCGTCGCG CGAACCCAGG GCCACCAGCA 961 GTTGCGTGGT GGTGGTTTTC CCCATCCCGT GAGGACCGTC TATATAAACC CGCAGTAGCG 1021 TGGGCATTTT TCTGCTCCAG GCGGACTTCC GTGGCTTCTT GCTGCCCGGC GAGGGCGCAC 1081 GCGTACGTCG GTTTGCTATG ACGCGAGACG CGCAGCTTGA TTCGAAACGC AGACGCCGAT 1141 GTTTGGAATT GC //
Primer: PGKpA_R Sequence file: RDB15400_A7Iec.seq
>01692_15400_A7Ie_PGKpA_R_B01_02_ABI08.ab1 1 GGGCCTAACT TCTTACAAGT ATGACAGGAA AACTTCCATT TTAGTGGACA TCTTTATTGT 61 TTAATAGATC ATCAATTTCT GCAGGTCCTG GCCGGGGTCC CGCGGAAACT CGGCCGTGGT 121 GACCAATACA AAACAAAAGC GCTCCTCGTA CCAGCGAAGA AGGGGCAGAG ATGCCGTAGT 181 CAGGTTTAGT TCGTCCGGCG GCGCCAGAAA TCCGCGCGGT GGTTTTTGGG GGTCGGGGGT 241 GTTTGGCAGC CACAGACGCC CGGTGTTCGT GTCGCGCCAG TACATGCGGT CCATGCCCAG 301 GCCATCCAAA AACCATGGGT CTGTCTGCTC AGTCCAGTCG TGGACCTGAC CCCACGCAAC 361 GCCCAAAATA ATAACCCCCA CGAACCATAA ACCATTCCCC ATGGGGGACC CCGTCCCTAA 421 CCCACGGGGC CCGTGGCTAT GGCAGGGCTT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG 481 CCTTCACCCG AACTTGGGGG GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC 541 CAATGGGGTC TCGGTGGGGT ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA 601 TGAACAAACG ACCCAACACC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 661 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCATC TCCCGGGCAA 721 ACGTGCGCGC CAGGTCGCAG ATCGTCGGTA TGGAGCCGGG GGTGGTGACG TGGGTCTGGA 781 CCATCCCGGA GGTAAGTTGC AGCAGGGCGT CCCGGCAGCC GGCGGGCGAT TGGTCGTAAT 841 CCAGGATAAA GACGTGCATG GGGACGGAGG CGTTTGGCCA AGACGTCCAA GGCCCAGGCA 901 AACACGTTGT ACAGGTCGCC GTTGGGGGCC AGCAACTCGG GGGGCCCGAA ACAGGGTAAA 961 TAACGTGTCC CCGATATGGG GTCGTGGGCC CGCGTTGCTC TGGGGCTTCG GCACCCTGGG 1021 GGCGGGCACG GCCGTTCCCC CGAAAAGCTG TCCCAATCCT CCCGGCCACG GACCCGCCCG 1081 CCTCCTGCCA GGATACCGGC ACCGTAATGG GCAAGGCCAG CCCGTTAACG CGGCGAAATC 1141 CGCCGCCAGC CAATAGACTA AGGTTCAAAG CTCAGTCCTT CGCTGT //
Primer: SV40pA3'Element Reverse Sequence file: RDB15400_A7Ied.seq
>01692_15400_A7Ie_SV40pA3'ElementReverse_B11_05_ABI24.ab1 1 GACTCAAGGG GGGGCGGACC TATGGAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG 61 GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT 121 AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC 181 AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCCAATAC GCAAACCGCC TCTCCCCGCG 241 CGTTGGCCGA TTCATTAATG CAGCTGAACG GTCTGGTTAT AGGTACATTG AGCAACTGAC 301 TGAAATGCCT CAAAATGTTC TTTACGATGC CATTGGGATA TATCAACGGT GGTATATCCA 361 GTGATTTTTT TCTCCATTTT AGCTTCCTTA GCTCCTGAAA ATCTCGCCAA GCTTCTGATG 421 GAATTAGAAC TTGGCAAAAC AATACTGAGA ATGAAGTGTA TGTGGAACAG AGGCTGCTGA 481 TCTCGTTCTT CAGGCTATGA AACTGACACA TTTGGAAACC ACAGTACTTA GAACCACAAA 541 GTGGGAATCA AGAGAAAAAC AATGATCCCA CGAGAGATCT ATAGATCTAT AGATCATGAG 601 TGGGAGGAAT GAGCTGGCCC TTAATTTGGT TTTGCTTGTT TAAATTATGA TATCCAACTA 661 TGAAACATTA TCATAAAGCA ATAGTAAAGA GCCTTCAGTA AAGAGCAGGC ATTTATCTAA 721 TCCCACCCCA CCCCCACCCC CGTAGCTCCA ATCCTTCCAT TCAAAATGTA GGTACTCTGT 781 TCTCACCCTT CTTAACAAAG TATGACAGGA AAAACTTCCA TTTTAGTGGA CATCTTTATT 841 GTTTAATAGA TCATCAATTT CTGCAGGTCC TGGCCGGGGT CCCGCGGAAA CTCGGCCGTG 901 GTGACCAATA CAAAACAAAA GCGCTCCTCG TACCAGCGAA GAAAGGGCAG AGATGCCGTA 961 GTCAGGTTTA GTTCGTCCGG CGGCGCCAGA AATCCGCGCG GTGGGTTTTT GGGGGGTCGG 1021 GGGGGTGTTT GGCAGCCACA GACGCCCGGG TGTTCGTGTC GCGCCAGTAC ATGCGGGTCC 1081 AATGCCCAGG CCCATCCAAA ATCATGGGTC CTGTCCTGCT TCAGGTTCCA GTCGGTGGAC 1141 CTGGACCCCC CACGGCCA //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

user_report	Kawasaki M., Inducible liver injury in the transgenic rat by expressing liver-specific suicide gene. Biochem. Biophys. Res. Commun., 311, 920-928 (2003). PMID 14623269. [link to RRC of NBRP]
user_report	Mizuguchi H., Enhanced antitumor effect and reduced vector dissemination with fiber-modified adenovirus vectors expressing herpes simplex virus thymidine kinase. Cancer Gene Ther., 9, 236-242 (2002). PMID 11896439. [link to RRC of NBRP]