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AxCALacZ (#RDB01745)

CAG promoter + lacZ

Clone info. A recombinant adenovirus expressing beta-galactosidase (lacZ) under control of CAG promoter.
Vector backbone pAxcw (Adenovirus)
Size of vector backbone -
Growth remarks 5 percent FCS is fine.
Gene/insert name E. coli lacZ (beta-D-galactosidase) Genomic DNA
Depositor|Developer Saito, Izumu |
 
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Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Kanegae, Y., Nucleic Acids Res., 23, 3816-3821(1995)).
Additional terms and conditions:
Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
Remarks Please ensure RCAs are not in stocks prior to use of recombinant adenoviruses. (https://brc.riken.jp/dna/GRP0046e)
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
遺伝子組換え生物の受入れ確認書 [Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Kanegae, Y., Nucleic Acids Res., 23, 3816-3821(1995))。
MTAに書く付加的使用条件：
CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。
備考 【追加提供依頼書】受入れ確認書(書式J)
実験に使用する前に、タイターを上げた試料中にRCAが混入していないことを確認してください。(https://brc.riken.jp/dna/GRP0046j)
組換え体提供にかかる書式が必要です。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB01745 AxCALacZ Under QC test. Please contact us. Virus, lysate of infected cell culture JPY 32,340 plus cost of shipping containers, dry ice (if required) and shipping charge

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The AxCALacZ was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB01745).

Reference section:

Kanegae, Y., Lee, G., Sato, Y., Tanaka, M., Nakai, M., Sakaki, T., Sugano, S., Saito, I., Efficient gene activation in mammalian cells by using recombinant adenovirus expressing site-specific Cre recombinase. Nucleic Acids Res., 23, 3816-3821 (1995). PMID 7479022. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

References

Original, user report and related articles

original Kanegae, Y., Efficient gene activation in mammalian cells by using recombinant adenovirus expressing site-specific Cre recombinase. Nucleic Acids Res., 23, 3816-3821 (1995). PMID 7479022. [link to RRC of NBRP]
reference Niwa, H., Efficient selection for high-expression transfectants with a novel eukaryotic vector. Gene, 108 (2): 193-200 (1991). PMID 1660837. [link to RRC of NBRP]
user_report Shen, Y.H., Arg-Gly-Asp (RGD)-Modified E1A/E1B Double Mutant Adenovirus Enhances Antitumor Activity in Prostate Cancer Cells In Vitro and in Mice. PLoS One. 11 (1): e0147173 (2016). PMID 26799485. [link to RRC of NBRP]
user_report Kelloniemi, A., TSC-22 up-regulates collagen 3a1 gene expression in the rat heart. BMC Cardiovasc. Disord. 15 (1): 122 (2015). PMID 26464165. [link to RRC of NBRP]
user_report Fukuda K., E1A, E1B double-restricted replicative adenovirus at low dose greatly augments tumor-specific suicide gene therapy for gallbladder cancer. Cancer Gene Ther., 16, 126-136 (2009). PMID 18818710. [link to RRC of NBRP]
user_report Suetsugu A., Differentiation of mouse hepatic progenitor cells induced by hepatocyte nuclear factor-4 and cell transplantation in mice with liver fibrosis. Transplantation, 86, 1178-1186 (2008). PMID 19005397. [link to RRC of NBRP]
user_report Nakade K., JDP2 suppresses adipocyte differentiation by regulating histone acetylation. Cell Death Differ., 14, 1398-1405 (2007). PMID 17464331. [link to RRC of NBRP]
user_report Wakayama M., E1A, E1B double-restricted adenovirus with RGD-fiber modification exhibits enhanced oncolysis for CAR-deficient biliary cancers. Clin. Cancer Res., 13, 3043-3050 (2007). PMID 17505007. [link to RRC of NBRP]
user_report Nakayama Y., CTLA-4Ig suppresses liver injury by inhibiting acquired immune responses in a mouse model of fulminant hepatitis. Hepatology, 42, 915-924 (2006). PMID 16175605. [link to RRC of NBRP]
user_report Lee J.H., Suppression of PTEN expression during aggregation with retinoic acid in P19 mouse embryonal carcinoma cells. Biochem. Biophys. Res. Commun., 347, 715-722 (2006). PMID 16842746. [link to RRC of NBRP]
user_report Seo E., Effective gene therapy of biliary tract cancers by a conditionally replicative adenovirus expressing uracil phosphoribosyltransferase: significance of timing of 5-fluorouracil administration. Cancer Res., 65, 546-552 (2005). PMID 15695398. [link to RRC of NBRP]
user_report Nishida M., G alpha 12/13- and reactive oxygen species-dependent activation of c-Jun NH2-terminal kinase and p38 mitogen-activated protein kinase by angiotensin receptor stimulation in rat neonatal cardiomyocytes. J. Biol. Chem., 280, 18434-18441 (2005). PMID 15743761. [link to RRC of NBRP]
user_report Fujii T., Galpha12/13-mediated production of reactive oxygen species is critical for angiotensin receptor-induced NFAT activation in cardiac fibroblasts. J. Biol. Chem., 280, 23041-23047 (2005). PMID 15826947. [link to RRC of NBRP]
user_report Kojima Y., Adenovirus-mediated p53 gene transfer to the bile duct by direct administration into the bile in a rat cholangitis model. J. Surg. Res., 128, 126-131 (2005). PMID 16005898. [link to RRC of NBRP]
user_report Kojima Y., Adenovirus-mediated p53 gene transfer to the biliary tract in a rat cholangitis model. J. Japan Biliary Assoc., 18, 174-181 (2004). [link to RRC of NBRP]
user_report Inoshima I., Induction of CDK inhibitor p21 gene as a new therapeutic strategy against pulmonary fibrosis. Am. J. Physiol. Lung Cell. Mol. Physiol., 286, L727-L733 (2004). PMID 15003936. [link to RRC of NBRP]
user_report Miao G., Development of donor-specific immunoregulatory T-cells after local CTLA4Ig gene transfer to pancreatic allograft. Transplantation, 78, 59-64 (2004). PMID 15257039. [link to RRC of NBRP]
user_report Naiki T., Functional activity of human hepatoma cells transfected with adenovirus-mediated hepatocyte nuclear factor (HNF)-4 gene. Cell Transplant., 13, 393-403 (2004). PMID 15468681. [link to RRC of NBRP]
user_report Arai K., Differential Requirement of G{alpha}12, G{alpha}13, G{alpha}q, and G{beta gamma} for Endothelin-1-Induced c-Jun NH2-Terminal Kinase and Extracellular Signal-Regulated Kinase Activation. Mol. Pharmacol., 63, 478-488 (2003). PMID 12606754. [link to RRC of NBRP]
user_report Iwaki T., Recombinant adenovirus vector bearing antisense macrophage migration inhibitory factor cDNA prevents acute lipopolysaccharide-induced liver failure in mice. Lab. Invest., 83, 561-570 (2003). PMID 12695559. [link to RRC of NBRP]
user_report Akamaru Y., Ex vivo and systemic transfer of adenovirus-mediated CTLA4Ig gene combined with a short course of FK506 therapy prolongs islet graft survival. Transpl. Immunol., 11, 91-100 (2003). PMID 12727480. [link to RRC of NBRP]
user_report Yazawa M., Effect of an adenoviral vector that expresses the canine p53 gene on cell growth of canine osteosarcoma and mammary adenocarcinoma cell lines. Am. J. Vet. Res., 64, 880-888 (2003). PMID 12856773. [link to RRC of NBRP]
user_report Kanatsu-Shinohara M., Adenovirus-mediated gene delivery and in vitro microinsemination produce offspring from infertile male mice. Proc. Natl. Acad. Sci. U. S. A., 99, 1383-1388 (2002). PMID 11818536. [link to RRC of NBRP]
user_report Furumoto K., Interleukin-12-gene transduction makes DCs from tumor-bearing mice an effective inducer of tumor-specific immunity in a peritoneal dissemination model. Immunol. Lett., 83, 13-20 (2002). PMID 12057850. [link to RRC of NBRP]
user_report Hirai S., MAPK-upstream protein kinase (MUK) regulates the radial migration of immature neurons in telencephalon of mouse embryo. Development, 129, 4483-4495 (2002). PMID 12223406. [link to RRC of NBRP]
user_report Nagai-Tamai Y., Regulated protein-protein interaction between aPKC and PAR-3 plays an essential role in the polarization of epithelial cells. Genes Cells, 7, 1161-1171 (2002). PMID 12390250. [link to RRC of NBRP]
user_report Maruyama Y., Galpha(12/13) mediates alpha(1)-adrenergic receptor-induced cardiac hypertrophy. Circ. Res., 91, 961-969 (2002). PMID 12433842. [link to RRC of NBRP]
user_report Ozawa K., Expression of the oxygen-regulated protein ORP150 accelerates wound healing by modulating intracellular VEGF transport. J. Clin. Invest., 108, 41-50 (2001). PMID 11435456. [link to RRC of NBRP]
user_report Kobayashi, N., Successful retroviral gene transfer of simian virus 40 T antigen and herpes simplex virus-thymidine kinase into human hepatocytes. Cell Transplant, 10 (4-5): 377-381 (2001). PMID 11549057. [link to RRC of NBRP]
user_report Kobayashi N., Cre/loxP-based reversible immortalization of human hepatocytes. Cell Transplant., 10, 383-386 (2001). PMID 11549058. [link to RRC of NBRP]
user_report Kobayashi N., Rapidly functional immobilization of immortalized human hepatocytes using cell adhesive GRGDS peptide-carrying cellulose microspheres. Cell Transplant., 10, 387-392 (2001). PMID 11549059. [link to RRC of NBRP]
user_report Kobayashi N., Prevention of acute liver failure in rats with reversibly immortalized human hepatocytes. Science, 287, 1258-1262 (2000). PMID 10678831. [link to RRC of NBRP]
user_report Ohsawa T., Enhancement of adenovirus-mediated gene transfer into dermal fibroblasts in vitro and in vivo by polyethylene glycol 6000. J. Dermatol., 27, 244-251 (2000). PMID 10824488. [link to RRC of NBRP]
user_report Nishida M., G alpha(i) and G alpha(o) are target proteins of reactive oxygen species. Nature, 408, 492-495 (2000). PMID 11100733. [link to RRC of NBRP]
user_report Tatebe S., Adenovirus-mediated transfer of wild-type p53 gene results in apoptosis or growth arrest in human cultured gastric carcinoma cells. Int. J. Oncol., 15, 229-235 (1999). PMID 10402231. [link to RRC of NBRP]
user_report Nakamura T., Hepatoprotective action of adenovirus-transferred HNF-3gamma gene in acute liver injury caused by CCl4. FEBS Let., 459, 1-4 (1999). PMID 10508906. [link to RRC of NBRP]
user_report Nakamura T., Adenovirus-mediated gene expression in the septal cells of cirrhotic rat livers. J. Hepatol., 30, 101-106 (1999). PMID 9927156. [link to RRC of NBRP]
user_report Nakamura T., Adenovirus-transferred HNF-3 gamma conserves some liver functions in primary cultured hepatocytes of adult rats. Biochem. Biophys. Res. Commun., 253, 352-357 (1998). PMID 9878541. [link to RRC of NBRP]
user_report Kanamori Y., A newly developed adenovirus-mediated transfer of a wild-type p53 gene increases sensitivity to cis-diamminedichloroplatinum (II) in p53-deleted ovarian cancer cells. Eur. J. Cancer., 34, 1802-1806 (1998). PMID 9893672. [link to RRC of NBRP]