RDB01886 - pCAhCTLA4Ig

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

pCAhCTLA4Ig (#RDB01886)

Expression clone of human CTLA4 and IgG1

Alternative name	pCAccCTLA4Ig
Clone info.	Expression clone of uman CTLA4 and IgG1.
Vector backbone	pCAcc (Plasmid)
Size of vector backbone	4.7 kb
Selectable markers	Amp^r
Gene/insert name	Human CTLA4 cDNA Human cDNA
Depositor\|Developer	Hamada, Hirofumi \|

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Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link]

Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.(Science, 245, 631-635 (1989).) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Additional terms and conditions:
Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.(Science, 245, 631-635 (1989).) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. Additional terms and conditions: Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	非営利機関の非営利学術研究へ提供する。利用者が本件リソースを利用した研究成果に基づき特許申請、商業活動等を行う場合は、利用者は事前に寄託者に連絡する。利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Science, 245, 631-635 (1989).)。利用者は、研究成果の公表にあたって謝辞の表明を必要とする。 MTAに書く付加的使用条件： CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学宮崎純一博士への謝辞の表明を必要とする。

必要書類

提供依頼書

[依頼書の記入例

]
提供同意書 (MTA, 非営利学術目的用)[Word

] [MTAの記入例

]
手続きの概要は、「提供申込みについて[link]」をご覧ください。

MTAに書く使用条件

非営利機関の非営利学術研究へ提供する。利用者が本件リソースを利用した研究成果に基づき特許申請、商業活動等を行う場合は、利用者は事前に寄託者に連絡する。利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Science, 245, 631-635 (1989).)。利用者は、研究成果の公表にあたって謝辞の表明を必要とする。
MTAに書く付加的使用条件：
CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学宮崎純一博士への謝辞の表明を必要とする。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB01886	pCAhCTLA4Ig	DNA solution	Please contact us.

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCAhCTLA4Ig was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB01886).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB12738_A4Bs.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCAF1 Sequence file: RDB12738_A4Bsa.seq
>01886_12738_A4Bs_pAxCAF1_A11_02.ab1 1 TCGGCGGGGC GGAGTACGAT CTTCTCATGT TATGCCTTCT TCTTTTTCCT ACAGCTCCTG 61 GGCAACGTGC TGGTTATTGT GCTGTCTCAT CATTTTGGCA AAGAATTCGA TAAGCTTCAC 121 CATGGGTGTA CTGCTCACAC AGAGGACGCT GCTCAGTCTG GTCCTTGCAC TCCTGTTTCC 181 AAGCATGGCG AGCATGGCAA TGCACGTGGC CCAGCCTGCT GTGGTACTGG CCAGCAGCCG 241 AGGCATCGCC AGCTTTGTGT GTGAGTATGC ATCTCCAGGC AAAGCCACTG AGGTCCGGGT 301 GACAGTGCTT CGGCAGGCTG ACAGCCAGGT GACTGAAGTC TGTGCGGCAA CCTACATGAT 361 GGGGAATGAG TTGACCTTCC TAGATGATTC CATCTGCACG GGCACCTCCA GTGGAAATCA 421 AGTGAACCTC ACTATCCAAG GACTGAGGGC CATGGACACG GGACTCTACA TCTGCAAGGT 481 GGAGCTCATG TACCCACCGC CATACTACCT GGGCATAGGC AACGGAACCC AGATTTATGT 541 AATTGATCCA GAACCGTGCC CAGATTCTGA TCAGGAGCCC AAATCTTCTG ACAAAACTCA 601 CACATCCCCA CCGTCCCCAG CACCTGAACT CCTGGGGGGA CCGTCAGTCT TCCTCTTCCC 661 CCCAAAACCC AAGGACACCC TCATGATCTC CCGGACCCCT GAGGTCACAT GCGTGGTGGT 721 GGACGTGAGC CACGAAGACC CTGAGGTCAA GTTCAACTGG TACGTGGACG GCGTGGAGGT 781 GCATAATGCC AAGACAAAGC CGCGGGAGGA GCAGTACAAC AGCACGTACC GTGTGGTCAG 841 CGTCCTCACC GTCCTGCACC AGGACTGGCT GAATGGCAAG GAGTACAAGT GCAAGGTCTC 901 CAACAAAGCC CTCCCAGCCC CCATCGAGAA AACCATCTCC AAAGCCAAAG GGCAGCCCCG 961 AGAACCACAG GTGTACACCC TGCCCCCATC CCGGGATGAG CTGACCAAGA AACCAGGGTC 1021 AGCCCTGACC CTGCCCTGGT CAAGGCTTCT ATCCCAGCGA CATCGCCCGT GGAGTGGGAG 1081 AGCAATGGGG CAGCCCGGGA GAACACTACT AGGACACGGC ATCCGATGCC TGACTCCGAC 1141 GGCCTTCCTT TCTTTCCCTC C //
Primer: pAxCA_R1 Sequence file: RDB12738_A4Bsb.seq
>01886_12738_A4Bs_pAxCAR1_B11_05.ab1 1 TTTGGGGAAA AGGGCATTGG CCACACCAGC CACCACCTTC TGATAGGCAG CCTGCACCTG 61 AGGAGTGAAT TCTAGATTAT TTACCCGGAG ACAGGGAGAG GCTCTTCTGC GTGTAGTGGT 121 TGTGCAGAGC CTCATGCATC ACGGAGCATG AGAAGACGTT CCCCTGCTGC CACCTGCTCT 181 TGTCCACGGT GAGCTTGCTG TAGAGGAAGA AGGAGCCGTC GGAGTCCAGC ACGGGAGGCG 241 TGGTCTTGTA GTTGTTCTCC GGCTGCCCAT TGCTCTCCCA CTCCACGGCG ATGTCGCTGG 301 GATAGAAGCC TTTGACCAGG CAGGTCAGGC TGACCTGGTT CTTGGTCAGC TCATCCCGGG 361 ATGGGGGCAG GGTGTACACC TGTGGTTCTC GGGGCTGCCC TTTGGCTTTG GAGATGGTTT 421 TCTCGATGGG GGCTGGGAGG GCTTTGTTGG AGACCTTGCA CTTGTACTCC TTGCCATTCA 481 GCCAGTCCTG GTGCAGGACG GTGAGGACGC TGACCACACG GTACGTGCTG TTGTACTGCT 541 CCTCCCGCGG CTTTGTCTTG GCATTATGCA CCTCCACGCC GTCCACGTAC CAGTTGAACT 601 TGACCTCAGG GTCTTCGTGG CTCACGTCCA CCACCACGCA TGTGACCTCA GGGGTCCGGG 661 AGATCATGAG GGTGTCCTTG GGTTTTGGGG GGAAGAGGAA GACTGACGGT CCCCCCAGGA 721 GTTCAGGTGC TGGGGACGGT GGGGATGTGT GAGTTTTGTC AGAAGATTTG GGCTCCTGAT 781 CAGAATCTGG GCACGGTTCT GGATCAATTA CATAAATCTG GGTTCCGTTG CCTATGCCCA 841 GGTAGTATGG CGGTGGGTAC ATGAGCTCCA CCTTGCAGAT GTAGAGTCCC GTGTCCATGG 901 CCCTCAGTCC TTGGATAGTG AGGTTCACTT GATTTCCACT GGAGGTGCCC GTGCAGATGG 961 AATCATCTAG GAAGGTCAAC TCATTCCCCA TCATGTAGGT TGCCCGCACA GACTTCAGTC 1021 ACCTGGCTGT CAGCCCTGCC GAAGCACTGT CACCCGGACC TCAGTGCTTT GCCTGAGATG 1081 CATACTCACA CACAAGGCTG GGCGATGCTC GGCTGCTGGC AGGTTACACA GCAGCTTGGC 1141 ACGGGTGCAT TTGACAATGC AACT //