RDB01903 - pCAUT

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pCAUT (#RDB01903)

E. coli UPRTase + HSV-TK, genomic DNA

Alternative name	E. coli UPRTase + HSV-TK genomic DNA
Clone info.	Expression vector of E. coli UPRTase and HSV-TK fusion protein.
Vector backbone	pCAGGS (Plasmid)
Size of vector backbone	4.7 kb
Selectable markers	Amp^r (E. coli)
Gene/insert name	E. coli uracil phosphoribosyl transferase (UPRTase) Genomic DNA HSV thymidine kinase genomic DNA
Depositor\|Developer	Hamada, Hirofumi \|

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Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Additional terms and conditions:
Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. Additional terms and conditions: Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	非営利機関の非営利学術研究へ提供する。利用者が本件リソースを利用した研究成果に基づき特許申請、商業活動等を行う場合は、利用者は事前に寄託者に連絡する。利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する。利用者は、研究成果の公表にあたって謝辞の表明を必要とする。 MTAに書く付加的使用条件： CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学宮崎純一博士への謝辞の表明を必要とする。

必要書類

提供依頼書

[依頼書の記入例

]
提供同意書 (MTA, 非営利学術目的用)[Word

] [MTAの記入例

]
手続きの概要は、「提供申込みについて[link]」をご覧ください。

MTAに書く使用条件

非営利機関の非営利学術研究へ提供する。利用者が本件リソースを利用した研究成果に基づき特許申請、商業活動等を行う場合は、利用者は事前に寄託者に連絡する。利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する。利用者は、研究成果の公表にあたって謝辞の表明を必要とする。
MTAに書く付加的使用条件：
CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学宮崎純一博士への謝辞の表明を必要とする。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB01903	pCAUT	DNA solution	Please contact us.

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCAUT was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB01903).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB13439_A5Fi.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1 Sequence file: RDB13439_A5Fia.seq
>01903_13439_A5Fi_pAxCA_F1_F05_17.ab1 1 CTCGCCGAAG GAACCGAAAT TCTCATGTTC ATGCCTTCTT CTTTTTCCTA CAGCTCCTGG 61 GCAACGTGCT GGTTATTGTG CTGTCTCATC ATTTTGGCAA AGAATTCCAC CATGAAGATC 121 GTGGAAGTCA AACACCCACT CGTCAAACAC AAGCTGGGAC TGATGCGTGA GCAAGATATC 181 AGCACCAAGC GCTTTCGCGA ACTCGCTTCC GAAGTGGGTA GCCTGCTGAC TTACGAAGCG 241 ACCGCCGACC TCGAAACGGA AAAAGTAACT ATCGAAGGCT GGAACGGCCC GGTAGAAATC 301 GACCAGATCA AAGGTAAGAA AATTACCGTT GTGCCAATTC TGCGTGCGGG TCTTGGTATG 361 ATGGACGGTG TGCTGGAAAA CGTTCCGAGC GCGCGCATCA GCGTTGTCGG TATGTACCGT 421 AATGAAGAAA CGCTGGAGCC GGTACCGTAC TTCCAGAAAC TGGTTTCTAA CATCGATGAG 481 CGTATGGCGC TGATCGTTGA CCCAATGCTG GCAACCGGTG GTTCCGTTAT CGCGACCATC 541 GACCTGCTGA AAAAAGCGGG CTGCAGCAGC ATCAAAGTTC TGGTGCTGGT AGCTGCGCCA 601 GAAGGTATCG CTGCGCTGGA AAAAGCGCAC CCGGACGTCG AACTGTATAC CGCATCGATT 661 GATCAGGGAC TGAACGAGCA CGGATACATT ATTCCGGGCC TCGGCGATGC CGGTGACAAA 721 ATCTTTGGTA CGAAAGGAGG ATCCGGCGGG GGCGGTGGAG GAGGGGGTAT GGCTTCGTAC 781 CCCTGCCATC AACACGCGTC TGCGTTCGAC CAGGCTGCGC GTTCTCGCGG CCATAGCAAC 841 CGACGTACGG CGTTGCGCCC TCGCCGGCAG CAAGAAGCCA CGGAAGTCCG CCTGGAGCAG 901 AAAATGCCCA CGCTACTGCG GGTTTATATA GACGGTCCTC ACGGGATGGG GAAAACCACC 961 ACCACGCAAC TGCTGGTGGC CCTGGGTTCG CGCGACGATA TCGTCTACGT ACCCGAGCCG 1021 ATGACTTACT GGCAGGTGCT GGGGGCTTCC GAGACAATCG CGAACATCTA CACCACACAA 1081 CACCGCCTCG ACACGTGAGA TATCGGCCGG GGGACGCGCC GTTGTATGAC AGCGCCCAGA 1141 ATACATGGGC ATGCCTTATT GCCCGTGAAC CGAACGCCCG ATTG //
Primer: HSV TK pA_R Sequence file: RDB13439_A5Fib.seq
>01903_13439_A5Fi_HSVTKpA_R_H09_24.ab1 1 AGGGGGATCT TCGGTATTGT CTCCTTCCGT GTTTCAGTTA GCCTCCCCCA TCTCCCGGGC 61 AAACGTGCGC GCCAGGTCGC AGATCGTCGG TATGGAGCCG GGGGTGGTGA CGTGGGTCTG 121 GACCATCCCG GAGGTAAGTT GCAGCAGGGC GTCCCGGCAG CCGGCGGGCG ATTGGTCGTA 181 ATCCAGGATA AAGACGTGCA TGGGACGGAG GCGTTTGGCC AAGACGTCCA AGGCCCAGGC 241 AAACACGTTG TACAGGTCGC CGTTGGGGGC CAGCAACTCG GGGGCCCGAA ACAGGGTAAA 301 TAACGTGTCC CCGATATGGG GTCGTGGGCC CGCGTTGCTC TGGGGCTCGG CACCCTGGGG 361 CGGCACGGCC GTCCCCGAAA GCTGTCCCCA ATCCTCCCGC CACGACCCGC CGCCCTGCAG 421 ATACCGCACC GTATTGGCAA GCAGCCCGTA AACGCGGCGA ATCGCGGCCA GCATAGCCAG 481 GTCAAGCCGC TCGCCGGGGC GCTGGCGTTT GGCCAGGCGG TCGATGTGTC TGTCCTCCGG 541 AAGGGCCCCC AACACGATGT TTGTGCCGGG CAAGGTCGGC GGGATGAGGG CCACGAACGC 601 CAGCACGGCC TGGGGGGTCA TGCTGCCCAT AAGGTATCGC GCGGCCGGGT AGCACAGGAG 661 GGCGGCGATG GGATGGCGGT CGAAGATGAG GGTGAGGGCC GGGGGCGGGG CATGTGAGCT 721 CCCAGCCTCC CCCCCGATAT GAGGAGCCAG AACGGCGTCG GTCACGGCAT AAGGCATGCC 781 CATTGTTATC TGGGCGCTTG TCATTACCAC CGCCGCGTCC CCGGCCGATA TCTCACCCTG 841 GTCGAGGCGG TGTTGTGTGG TGTAGATGTT CGCGATTGTC TCGGAAGCCC CCAGCACCTG 901 CCAGTAAGTC ATCGGCTCGG GTACGTAGAC GATATCGTCG CGCGAACCCA GGGCCACCAG 961 CAGTTGCGTG GTGGTGGTTT TCCCCATCCC GTGAGGACCG TCTATATAAA CCCGCAGTAG 1021 CGTGGGCATT TTCTGCTCCA GGCGGGACTT CCGTGGCTTC TTGCTGCCGG CGAGGGCGCA 1081 ACGCCCGTAC GTCGCTTGCT ATGGCCGCGA GAACGCGCAG CTTATTCGGA CGGCAGACGC 1141 CGTGTTGATG GCCAAGGGGA TAACGAAGCC ATATACCCCC CCATCGT //
Primer: Reverse2 Sequence file: RDB13439_A5Fic.seq
>01903_13439_A5Fi_Reverse2_G09_21.ab1 1 AATACCTGAT TACGCCAGCT TCTGATGGAA TTAGAACTTG GCAAAACAAT ACTGAGAATG 61 AAGTGTATGT GGAACAGAGG CTGCTGATCT CGTTCTTCAG GCTATGAAAC TGACACATTT 121 GGAAACCACA GTACTTAGAA CCACAAAGTG GGAATCAAGA GAAAAACAAT GATCCCACGA 181 GAGATCTATA GATCTATAGA TCATGAGTGG GAGGAATGAG CTGGCCCTTA ATTTGGTTTT 241 GCTTGTTTAA ATTATGATAT CCAACTATGA AACATTATCA TAAAGCAATA GTAAAGAGCC 301 TTCAGTAAAG AGCAGGCATT TATCTAATCC CACCCCACCC CCACCCCCGT AGCTCCAATC 361 CTTCCATTCA AAATGTAGGT ACTCTGTTCT CACCCTTCTT AACAAAGTAT GACAGGAAAA 421 ACTTCCATTT TAGTGGACAT CTTTATTGTT TAATAGATCA TCAATTTCTG CAGGTCCTGG 481 CCGGGGTCCC GCGGAAACTC GGCCGTGGTG ACCAATACAA AACAAAAGCG CTCCTCGTAC 541 CAGCGAAGAA GGGGCAGAGA TGCCGTAGTC AGGTTTAGTT CGTCCGGCGG CGCCAGAAAT 601 CCGCGCGGTG GTTTTTGGGG GTCGGGGGTG TTTGGCAGCC ACAGACGCCC GGTGTTCGTG 661 TCGCGCCAGT ACATGCGGTC CATGCCCAGG CCATCCAAAA ACCATGGGTC TGTCTGCTCA 721 GTCCAGTCGT GGACCTGACC CCACGCAACG CCCAAAATAA TAACCCCCAC GAACCATAAA 781 CCATTCCCCA TGGGGGACCC CGTCCCTAAC CCACGGGGCC CGTGGCTATG GCAGGGCTTG 841 CCGCCCCGAC GTTGGCTGCG AGCCCTGGGG CCTTCACCCG AACTTGGGGG GTGGGGGTGG 901 GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG GGTATCGACA 961 GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC ACCCGTGCGT 1021 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TTCCGGTATT GTCTCTTTCC 1081 GTGTTTCAGT TTAGCCTCCC CCATCTCCGG GCAACGTGGC GCGCAGGTTC GCAGATCGTT 1141 CGGGTGGAAG CCCGGGGGTG GTGACGGTGG GGTTCTTGGA CCCCAATC //
Primer: SV40pA3'Element Reverse Sequence file: RDB13439_A5Fid.seq
>01903_13439_A5Fi_SV40pA3'ElementReverse_F12_18.ab1 1 GCTCAGAGGG CGGAGCCTAT GGAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT 61 TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC 121 GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG 181 AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC CAATACGCAA ACCGCCTCTC CCCGCGCGTT 241 GGCCGATTCA TTAATGCAGC GGATCCAGAC ATGATAAGAT ACATTGATGA GTTTGGACAA 301 ACCACAACTA GAATGCAGTG AAAAAAATGC TTTATTTGTG AAATTTGTGA TGCTATTGCT 361 TTATTTGTAA CCATTATAAG CTGCAATAAA CAAGTTAGCT TTTTGCAAAA GCCTAGGCCT 421 CCAAAAAAGC CTCCTCACTA CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT 481 GCATAAATAA AAAAAATTAG TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT 541 AGGGGCGGGA TGGGCGGAGT TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCGGA 601 TCCGCTGGCA CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATG 661 TGAGTTAGCT CACTCATTAG GCACCCCAGG CTTTACACTT TATGCTTCCG GCTCGTATGT 721 TGTGTGGAAT TGTGAGCGGA TAACAATTTC ACACAGGAAA CAGCTATGAC CATGATTACG 781 CCAAGCTTCT GATGGAATTA GAACTTGGCA AAACAATACT GAGAATGAAG TGTATGTGGA 841 ACAGAGGCTG CTGATCTCGT TCTTCAGGCT ATGAAACTGA CACATTTGGA AACCACAGTA 901 CTTAGAACCA CAAAGTGGGA ATCAAGAGAA AAACAATGAT CCCACGAGAG ATCTATAGAT 961 CTATAGATCA TGAGTGGGAG GAATGAGCTG GCCCTTAATT TGGGTTTTGC TTGTTTAAAT 1021 TATGATATCC ACTATGAAAC ATTATCATAA GCATAGTAAG AGCTTCAGTA AGAGCAGCAT 1081 TTATCTATTC CACCCACCCC CACCCCCGTA GCTCAATCCT TCCAATTCAA TGTAAGGTAC 1141 TCTGGTTCTC ACCTTCTTAA CAAAGTATTG AAACAAGGGA //