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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pAMH10

E. coli - S. cerevisiae shuttle vector

Catalog number RDB01947
Resource name pAMH10
Alternative name E. coli-S. cerevisiae shuttle vector
Clone info. E. coli-S. cerevisiae shuttle vector, 9E10 epitope (Myc-tag), centromere-type ori, HIS3, ADH1 promoter, Ampicillin
Vector backbone pAMH10 (Plasmid)
Size of vector backbone 4,495 bp
Selectable markers Amp^r (E. coli), HIS3 (S. cerevisiae)
Depositor|Developer Iha, Hidekatsu |
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External Database

            Reference sequence
              
             
            Sequence RDB01947z.seq
            Remarks, protocol and/or map (gif) RDB01947.gif
            Remarks, protocol and/or map (gif) RDB01947z.gif

            Distribution information

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            RDB01947 pAMH10 DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            How to cite this biological resource

            Materials & Methods section:

            The pAMH10 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB01947).

            Reference section:

            Iha, H., Tsurugi, K., Shuttle-vector system for Saccharomyces cerevisiae designed to produce C-terminal-Myc-tagged fusion proteins. Biotechniques, 25, 936-938 (1998). PMID 9863043. [link to RRC of NBRP]

            Further references such as user reports and related articles (go to bottom)


            Tips

            Featured content

            Featured content Expression Vector Backbone of Saccharomyces cerevisiae (English text)

            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB13042_A4Lj.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: ADHrev
            Sequence file: RDB13042_A4Lja.seq check
            >01947_13042_A4Lj_ADHrev_G08_20.ab1
                1 GTGCTATCAG ATAAGATTTT CGTTTTAAAC CTAAGAGTCA CTTTAAAATT TGTATACACT
               61 TATTTTTTTT ATAACTTATT TAATAATAAA AATCATAAAT CATAAGAAAT TCGCCCGGAA
              121 TTAGCTTGGC TGCAGGTCGA CTCACAGATC CTCTTCGGAA ATCAGTTTCT GTTCAGAATT
              181 CGAGCTCGGT ACCCGGGGAT CCTCTAGAGT CAGCTTGGAG TTGATTGTAT GCTTGGTATA
              241 GCTTGAAATA TTGTGCAGAA AAAGAAACAA GGAAGAAAGG GAACGAGAAC AATGACGAGG
              301 AAACAAAAGA TTAATAATTG CAGGTCTATT TATACTTGAT AGCAAGACAG CAAACTTTTT
              361 TTTATTTCAA ATTCAAGTAA CTGGAAGGAA GGCCGTATAC CGTTGCTCAT TAGAGAGTAG
              421 TGTGCGTGAA TGAAGGAAGG AAAAAGTTTC GTGTGCTTCG AGATACCCCT CATCAGCTCT
              481 GGAACAACGA CATCTGTTGG TGCTGTCTTT GTCGTTAATT TTTTCCTTTA GTGTCTTCCA
              541 TCATTTTTTT GTCATTGCGG ATATGGTGAG ACAACAACGG GGGAGAGAGA AAAGAAAAAA
              601 AAAGAAAAGA AGTTGCATGC AAGCTCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA
              661 AACGCGCGAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA
              721 TAATGGTTTC TTAGGGTCCT TTTCATCACG TGCTATAAAA ATAATTATAA TTTAAATTTT
              781 TTAATATAAA TATATAAATT AAAAATAGAA AGTAAAAAAA GAAATTAAAG AAAAAATAGT
              841 TTTTGTTTTC CGAAGATGTA AAAGACTCTA GGGGGATCGC CAACAAATAC TACCTTTTAT
              901 CTTGCTCTTC CTGCTCTCAG GTATTAATGC CGAATTGTTT CATCTTGTCT GTGTAGAAGA
              961 CCACACACGA AAATCCTGTG ATTTTACATT TTACTTTATC GTTAATCGAA ATGTATATCT
             1021 ATTTAATCTG CTTTTCTGTC TAATTAAATA TATATGTAAG TACGCTTTTT TGTGAAATTT
             1081 TTAAACCTTT GATTATGTTT TCTTCATTCG GTAACTCGTC CTACCGTCTT TAATTTAACG
            1141 TTTTCTCATA
            //
            Primer: Amp_R
            Sequence file: RDB13042_A4Ljb.seq check
            >01947_13042_A4Lj_Amp_R_H08_23.ab1
                1 TCGTTTTCAT ATTATTGAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG
               61 AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC
              121 CTGACGGATC GCTTGCCTGT AACTTACACG CGCCTCGTAT CTTTTAATGA TGGAATAATT
              181 TGGGAATTTA CTCTGTGTTT ATTTATTTTT ATGTTTTGTA TTTGGATTTT AGAAAGTAAA
              241 TAAAGAAGGT AGAAGAGTTA CGGAATGAAG AAAAAAAAAT AAACAAAGGT TTAAAAAATT
              301 TCAACAAAAA GCGTACTTTA CATATATATT TATTAGACAA GAAAAGCAGA TTAAATAGAT
              361 ATACATTCGA TTAACGATAA GTAAAATGTA AAATCACAGG ATTTTCGTGT GTGGTCTTCT
              421 ACACAGACAA GATGAAACAA TTCGGCATTA ATACCTGAGA GCAGGAAGAG CAAGATAAAA
              481 GGTAGTATTT GTTGGCGATC CCCCTAGAGT CTTTTACATC TTCGGAAAAC AAAAACTATT
              541 TTTTCTTTAA TTTCTTTTTT TACTTTCTAT TTTTAATTTA TATATTTATA TTAAAAAATT
              601 TAAATTATAA TTATTTTTAT AGCACGTGAT GAAAAGGACC CTAAGAAACC ATTATTATCA
              661 TGACATTAAC CTATAAAAAT AGGCGTATCA CGAGGCCCTT TCGTCTCGCG CGTTTCGGTG
              721 ATGACGGTGA AAACCTCTGA CACATGCAGA GCTTGCATGC AACTTCTTTT CTTTTTTTTT
              781 CTTTTCTCTC TCCCCCGTTG TTGTCTCACC ATATCCGCAA TGACAAAAAA ATGATGGAAG
              841 ACACTAAAGG AAAAAATTAA CGACAAAGAC AGCACCAACA GATGTCGTTG TTCCAGAGCT
              901 GATGAGGGGT ATCTCGAAGC ACACGAAACT TTTTCCTTCC TTCATTCACG CACACTACTC
              961 TCTAATGAGC AACGGTATAC GGCCTTCCTT CCAGTTACTT GAATTTGAAA TAAAAAAAAA
             1021 GTTTGCTGTC TTGCTATCAA GTATAAAATA GACCTGCAGT ATAATCTTTT GTTTCCTTCG
             1081 TCATGTCTCG ATCCCTTTTC TTCTTGCTCC TTTTTCCTGG CACAATTATT TCAAGGCTTA
            1141 TTACCAAGGC AATTAACCAA ATTCCAA
            //
            Primer: HIS3pro_F
            Sequence file: RDB13042_A4Ljc.seq check
            >01947_13042_A4Lj_HIS3pro_F_A09_03.ab1
                1 TAGCAATTAT ACATTATATA AAGTAATGTG ATTTCTTCGA AGAATATACT AAAAAATGAG
               61 CAGGCAAGAT AAACGAAGGC AAAGATGACA GAGCAGAAAG CCCTAGTAAA GCGTATTACA
              121 AATGAAACCA AGATTCAGAT TGCGATCTCT TTAAAGGGTG GTCCCCTAGC GATAGAGCAC
              181 TCGATCTTCC CAGAAAAAGA GGCAGAAGCA GTAGCAGAAC AGGCCACACA ATCGCAAGTG
              241 ATTAACGTCC ACACAGGTAT AGGGTTTCTG GACCATATGA TACATGCTCT GGCCAAGCAT
              301 TCCGGCTGGT CGCTAATCGT TGAGTGCATT GGTGACTTAC ACATAGACGA CCATCACACC
              361 ACTGAAGACT GCGGGATTGC TCTCGGTCAA GCATTTAAAG AGGCCCTAGG GGCCGTGCGT
              421 GGAGTAAAAA GGTTTGGATC AGGATTTGCG CCTTTGGATG AGGCACTTTC CAGAGCGGTG
              481 GTAGATCTTT CGAACAGGCC GTACGCAGTT GTCGAACTTG GTTTGCAGAG GGAGAAAGTA
              541 GGAGATCTCT CTTGCGAGAT GATCCCGCAT TTTCTTGAAA GTTTTGCAGA GGCAAGCAGA
              601 ATTACCCTCC ACGTTGATTG TCTGCGAGGC AAGAATGATC ATCACCGTAG TGAGAGTGCG
              661 TTCAAGGCTC TTGCGGTTGC CATAAGAGAA GCCACCTCGC CCAATGGAAC CAACGATGTT
              721 CCCTCCACCA AAGGTGTTCT TATGTAGTGA CACCGATTAT TTAAAGCGGC AAGCTTACAT
              781 TTTATGTTAG CTGGTGGACT GACGCCAGAA AATGTTGGTG ATGCGCTTAG ATTAAATGGC
              841 GTTATTGGTG TTGATGTAAG CGGAGGTGTG GAGACAAATG GTGTAAAAGA CTCTAACAAA
              901 ATAGCAAATT TCGTCAAAAA TGCTAAGAAA TAGGTTATTA CTGAGTAGTA TTTATTTAAG
              961 TATTGTTTGT GCACTTGCCG GTAGAGGTGT GGTCAATAAG AGCGACCTCA TGCTATACCT
             1021 GAGAAGCAAC CCTGACCTAC AGGAAAGAGT TACTCAAGAA TAAGAATTTT CGTTTTAAAC
             1081 CTAGGAGTCA CTTTAAAATT TGTATACACT TATTTTTTTT TTTATACCTA TTATATAAAA
            1141 ATTCATTAAA TCCATAAGAA ATTCCGCCCG GGAATATAAG CCTGGGGCCT GC
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Iha, H., Shuttle-vector system for Saccharomyces cerevisiae designed to produce C-terminal-Myc-tagged fusion proteins. Biotechniques, 25, 936-938 (1998). PMID 9863043. [link to RRC of NBRP]

            2023.08.17

            GNP_filter3_RDBDEP_html_230702.pl