Resource data sheet
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pAML10 (#RDB01949)

E. coli - S. cerevisiae shuttle vector


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Iha, H., Biotechniques, 25, 936-938 (1998). PMID 9863043. [PubMed] [Article] [RRC of NBRP]
Test sheet Data Sheet open 
 
Alternative name E. coli-S. cerevisiae shuttle vector
Clone info. E. coli-S. cerevisiae shuttle vector, 9E10 epitope (Myc-tag), centromere-type ori, LEU2, ADH1 promoter, Ampicillin
Vector backbone pAML10 (Plasmid)
Size of vector backbone 5,124 bp
Selectable markers Amp^r (E. coli), LEU2 (S. cerevisiae)
Growth conditions 37 C, LB+Amp
Depositor|Developer Iha, Hidekatsu |

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution Acknowledgement to Depositor and Developer in publication.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
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MTAに書く使用条件 研究結果の発表時、謝辞に寄託者および樹立者名を入れること。

Catalog # Resource name Shipping form Fee
RDB01949 pAML10 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

How to cite this biological resource

Materials & Methods section:

The pAML10 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB01949).

Reference section:

Iha, H., Tsurugi, K., Shuttle-vector system for Saccharomyces cerevisiae designed to produce C-terminal-Myc-tagged fusion proteins. Biotechniques, 25, 936-938 (1998). PMID 9863043. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Iha, H., Shuttle-vector system for Saccharomyces cerevisiae designed to produce C-terminal-Myc-tagged fusion proteins. Biotechniques, 25, 936-938 (1998). PMID 9863043. [PubMed] [Article] [RRC of NBRP]