Please review the QC test results indicated by icon below as well as clone information before placing your order.

pGML10

E. coli - S. cerevisiae shuttle vector

Sequence	RDB01957z.seq
Remarks, protocol and/or map (gif)	RDB01957.gif
Remarks, protocol and/or map (gif)	RDB01957z.gif

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Please review the QC test results indicated by icon below as well as clone information before placing your order.

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB12344_A3Lk.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: ADHrev Sequence file: RDB12344_A3Lka.seq
>01957_12344_A3Lk_ADHrev_A12_03.ab1     1 TTAGGGATTA ATCAGGAATA AGATTTTCGT TTTAAACCTA AGAGTCACTT TAAAATTTGT    61 ATACACTTAT TTTTTTTATA ACTTATTTAA TAATAAAAAT CATAAATCAT AAGAAATTCG   121 CCCGGAATTA GCTTGGCTGC AGGTCGACTC ACAGATCCTC TTCGGAAATC AGTTTCTGTT   181 CAGAATTCGA GCTCGGTACC CGGGGATCCT CTAGAGTCAA TTCGATCCGG GGTTTTTTCT   241 CCTTGACGTT AAAGTATAGA GGTATATTAA CAATTTTTTG TTGATACTTT TATTACATTT   301 GAATAAGAAG TAATACAAAC CGAAAATGTT GAAAGTATTA GTTAAAGTGG TTATGCAGTT   361 TTTGCATTTA TATATCTGTT AATAGATCAA AAATCATCGC TTCGCTGATT AATTACCCCA   421 GAAATAAGGC TAAAAAACTA ATCGCATTAT CATCCTATGG TTGTTAATTT GATTCGTTCA   481 TTTGAAGGTT TGTGGGGCCA GGTTACTGCC AATTTTTCCT CTTCATAACC ATAAAAGCTA   541 GTATTGTAGA ATCTTTATTG TTCGGAGCAG TGCGGCGCGA GGCACATCTG CGTTTCAGGA   601 ACGCGACCGG TGAAGACGAG GACGCACGGA GGAGAGTCTT CCTTCGGAGG GCTGTCACCC   661 GCTCGGCGGC TTCTAATCCG TACTTCAATA TAGCAATGAG CAGTTAAGCG TATTACTGAA   721 AGTTCCAAAG AGAAGGTTTT TTTAGGCTAA GATAATGGGG CTCTTTACAT TTCCACAACA   781 TATAAGTAAG ATTAGATATG GATATGTATA TGGATATGTA TATGGTGGTA ATGCCATGTA   841 ATATGATTAT TAAACTTCTT TGCGTCCATC CAAAAAAAAA AGTAAGAATT TTTGAAAATT   901 CAATATAAAT GACAGCTCAG TTACAAAGTG AAAGTGACCT GCAGGCATGC AAGCTCTGCA   961 TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG ACGAAAGGGC CCTCGTGATA  1021 CGCCCTATTT TTTATAGGTT AATGTCATGA ATAATAAATG GTTTTCTTAG GGTCCTTTTC  1081 ATCACGTGCT AATAAAAATA TTATAATTTT AATTTTTTTA ATATCACTAT ATAAGTAAAA 1141 TAGGAAAGGT AAAAAAAGAA CTAAAAGCAA AAAATTAGGC TTCTG //
Primer: Amp_R Sequence file: RDB12344_A3Lkb.seq
>01957_12344_A3Lk_Amp_R_G10_19.ab1     1 TTGCTTCATA TTATTGAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA    61 ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC   121 TGACGGATCG CTTGCCTGTA ACTTACACGC GCCTCGTATC TTTTAATGAT GGAATAATTT   181 GGGAATTTAC TCTGTGTTTA TTTATTTTTA TGTTTTGTAT TTGGATTTTA GAAAGTAAAT   241 AAAGAAGGTA GAAGAGTTAC GGAATGAAGA AAAAAAAATA AACAAAGGTT TAAAAAATTT   301 CAACAAAAAG CGTACTTTAC ATATATATTT ATTAGACAAG AAAAGCAGAT TAAATAGATA   361 TACATTCGAT TAACGATAAG TAAAATGTAA AATCACAGGA TTTTCGTGTG TGGTCTTCTA   421 CACAGACAAG ATGAAACAAT TCGGCATTAA TACCTGAGAG CAGGAAGAGC AAGATAAAAG   481 GTAGTATTTG TTGGCGATCC CCCTAGAGTC TTTTACATCT TCGGAAAACA AAAACTATTT   541 TTTCTTTAAT TTCTTTTTTT ACTTTCTATT TTTAATTTAT ATATTTATAT TAAAAAATTT   601 AAATTATAAT TATTTTTATA GCACGTGATG AAAAGGACCC TAAGAAACCA TTATTATCAT   661 GACATTAACC TATAAAAATA GGCGTATCAC GAGGCCCTTT CGTCTCGCGC GTTTCGGTGA   721 TGACGGTGAA AACCTCTGAC ACATGCAGAG CTTGCATGCC TGCAGGTCAC TTTCACTTTG   781 TAACTGAGCT GTCATTTATA TTGAATTTTC AAAAATTCTT ACTTTTTTTT TGGATGGACG   841 CAAAGAAGTT TAATAATCAT ATTACATGGC ATTACCACCA TATACATATC CATATACATA   901 TCATATCTAT CTACTAATAT GTGGTGGAAA TGTAAGAGCT CCATTATTCT TAGCTAAAAA   961 AACTTCTTCT TTGGACTTCA GTATACCGCT ACTGCTCATG CTATATTGAG TACGATAAAG  1021 CGGCGACCGG TGAACGCCCT CGAGGAGACT CTCCTCGTGC GTCTCTCCTA CCGATCCGAT 1081 CTGAACGCAT GTGACCTCGG CGACTGATCC GA //
Primer: LEU2pro_F Sequence file: RDB12344_A3Lkc.seq
>01957_12344_A3Lk_LEU2pro_F_H10_22.ab1     1 CACTTGGCAG TTGGACAATA TTTATTTAAG GACCTATTGT TTTTTCCAAT TAGCCTGGTT    61 AGCAATCGTC TTACTTTCTA ACTTTTCTTA CCTTTTACAT TTCAGCAATA TATATATATA   121 TATTTCAAGG ATATACCATT CTAATGTCTG CCCCTAAGAA GATCGTCGTT TTGCCAGGTG   181 ACCACGTTGG TCAAGAAATC ACAGCCGAAG CCATTAAGGT TCTTAAAGCT ATTTCTGATG   241 TTCGTTCCAA TGTCAAGTTC GATTTCGAAA ATCATTTAAT TGGTGGTGCT GCTATCGATG   301 CTACAGGTGT TCCACTTCCA GATGAGGCGC TGGAAGCCTC CAAGAAGGCT GATGCCGTTT   361 TGTTAGGTGC TGTGGGTGGT CCTAAATGGG GTACAGGTAG TGTTAGACCT GAACAAGGTT   421 TACTAAAAAT CCGTAAAGAA CTTCAATTGT ACGCCAACTT AAGACCATGT AACTTTGCAT   481 CCGACTCTCT TTTAGACTTA TCTCCAATCA AGCCACAATT TGCTAAAGGT ACTGACTTCG   541 TTGTTGTCAG AGAATTAGTG GGAGGTATTT ACTTTGGTAA GAGAAAGGAA GACGATGGTG   601 ATGGTGTCGC TTGGGATAGT GAACAATACA CCGTTCCAGA AGTGCAAAGA ATCACAAGAA   661 TGGCCGCTTT CATGGCCCTA CAACATGAGC CACCATTGCC TATTTGGTCC TTGGATAAAG   721 CTAATGTTTT GGCCTCTTCA AGATTATGGA GAAAAACTGT GGAGGAAACC ATCAAGAACG   781 AATTTCCTAC ATTGAAGGTT CAACATCAAT TGATTGATTC TGCCGCCATG ATCCTAGTTA   841 AGAACCCAAA CCCACCTAAA TGGTATTATA ATCACCAGCA ACATGTTTGG TGATATCATC   901 TCCGATGAAG CCTCCGTTAT CCCAGGTTCC TTGGGTTTGT TGCCATCTGC GTCCTTGGCC   961 TCTTTGCCAG ACAAGAACAC CGCATTTGGG TTTGTACGAA CCATGCCACG GGTTTCTGCT  1021 CCAGATTTTG CCAAGAATAA GGTCAACCCC TATCGCCACT ATCTTGTCTG CTGCAATGAT  1081 GTTGAAAATG TCAATTGACT TGCTGAAGAG TAGCATGAAG ATGCCAGTAA AAGGTTTGGA 1141 TGCAAGGTAT ATCAAGAAAC ACAC //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.02

GNP_filter3_RDBDEP_html_230501.pl