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pAxCAhFL (#RDB02019)

Shuttle vector to generate rAd expressing human FAS Ligand

Clone info. Shuttle vector to generate recombinant adenovirus Human Fas Ligand expression. cDNA fragment 195 to 1126nt of NM_000639.2 (CDS, 195 to 1040nt) is cloned.
Comment Use the COS-TPC method for production of adenovirus. Known difference: C to T substitution at 756nt of NM_000639.2 (Leu, synonym).
Vector backbone pAxcw (Cosmid, use packaging extracts for transforming E. coli host)
Size of vector backbone 42.5 kb
Selectable markers Amp^r
Gene/insert name Human Fas ligand (FasL) cDNA
Depositor|Developer Hamada, Hirofumi |
 
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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR (Human Gene Therapy, 9, 2683-2689 (1998).) and an acknowledgment to the DEPOSITOR are requested.
Additional terms and conditions:
Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
Remarks Remember that you will be working with samples containing infectious virus.
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 非営利機関の非営利学術研究へ提供する。利用者が本件リソースを利用した研究成果に基づき特許申請、商業活動等を行う場合は、利用者は事前に寄託者に連絡する。利用者は、研究成果の公表にあたって寄託者の指定する文献 (Human Gene Therapy, 9, 2683-2689 (1998).) を引用し、謝辞の表明を必要とする。
MTAに書く付加的使用条件：
CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB02019 pAxCAhFL DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pAxCAhFL was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB02019).

Reference section:

Shinoura, N., Ohashi, M., Yoshida, Y., Asai, A., Kirino, T., Saito, I., Hamada, H., Construction, propagation, and titer estimation of recombinant adenoviruses carrying proapoptotic genes. Human Gene Therapy, 9, 2683-2689 (1998). PMID 9874266. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15036_A7Egp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1
Sequence file: RDB15036_A7Ega.seq check
>02019_15036_A7Eg_pAxCA_F1_F05_17_ABI24.ab1
    1 TCAAACCATG GTTTTCCTTG GCCTTTCTTT CTTTTTCCTA CAGCTCCTGG GCAACGTGCT
   61 GGTTATTGTG CTGTCTCATC ATTTTGGCAA AGAATTCGAT AAGCTTGCAT GCAGCAGCCC
  121 TTCAATTACC CATATCCCCA GATCTACTGG GTGGACAGCA GTGCCAGCTC TCCCTGGGCC
  181 CCTCCAGGCA CAGTTCTTCC CTGTCCAACC TCTGTGCCCA GAAGGCCTGG TCAAAGGAGG
  241 CCACCACCAC CACCGCCACC GCCACCACTA CCACCTCCGC CGCCGCCGCC ACCACTGCCT
  301 CCACTACCGC TGCCACCCCT GAAGAAGAGA GGGAACCACA GCACAGGCCT GTGTCTCCTT
  361 GTGATGTTTT TCATGGTTCT GGTTGCCTTG GTAGGATTGG GCCTGGGGAT GTTTCAGCTC
  421 TTCCACCTAC AGAAGGAGCT GGCAGAACTC CGAGAGTCTA CCAGCCAGAT GCACACAGCA
  481 TCATCTTTGG AGAAGCAAAT AGGCCACCCC AGTCCACCCC CTGAAAAAAA GGAGCTGAGG
  541 AAAGTGGCCC ATTTAACAGG CAAGTCCAAC TCAAGGTCCA TGCCTCTGGA ATGGGAAGAC
  601 ACCTATGGAA TTGTCCTGCT TTCTGGAGTG AAGTATAAGA AGGGTGGCCT TGTGATCAAT
  661 GAAACTGGGT TGTACTTTGT ATATTCCAAA GTATACTTCC GGGGTCAATC TTGCAACAAC
  721 CTGCCCCTGA GCCACAAGGT CTACATGAGG AACTCTAAGT ATCCCCAGGA TCTGGTGATG
  781 ATGGAGGGGA AGATGATGAG CTACTGCACT ACTGGGCAGA TGTGGGCCCG CAGCAGCTAC
  841 CTGGGGGGCA GTGTTCAATC TTACCAGTGC TGATCATTTA TATGTCAACG TATCTGAGCT
  901 CTCTCTGGTC AATTTTGAGG AATCTCAGAC GTTTTTCGGC TTATATAAGC TCTAAGAGAA
  961 GCACTTTGGA TTCTTTCCAT TATGATTCTT TGTTACAGGC ACCGAGAATG TTGTATTCGG
 1021 TGAGGTCTTC TTACATGCAG CCCGGGGGAT CCACTAGTTC TAGAATTCAC TCTCAAGGTG
 1081 CAGCCTGCCT ATTCAGAAGT GATGCTGGTG GTGCCCAAAT GCCTGGCTTC ACATACACTT
1141 GAGATTCTTT TTCCCCTCTT GCCAAAAATT ATGGGGAACA TCTATGTGA
//
Primer: pAxCA_R1
Sequence file: RDB15036_A7Egb.seq check
>02019_15036_A7Eg_1_pAxCA_R1_F07_16_ABI24.ab1
    1 CAAGTCAACA AACGAACCCT TTCTAGATAA AGCAGCCTGC ACCTGAGGAG TGAATTCTAG
   61 AACTAGTGGA TCCCCCGGGC TGCATGTAAG AAGACCCTCA CCGAATACAA CATTCTCGGT
  121 GCCTGTAACA AAGAATCATA ATGGAAAGAA TCCCAAAGTG CTTCTCTTAG AGCTTATATA
  181 AGCCGAAAAA CGTCTGAGAT TCCTCAAAAT TGACCAGAGA GAGCTCAGAT ACGTTGACAT
  241 ATAAATGATC AGCACTGGTA AGATTGAACA CTGCCCCCAG GTAGCTGCTG CGGGCCCACA
  301 TCTGCCCAGT AGTGCAGTAG CTCATCATCT TCCCCTCCAT CATCACCAGA TCCTGGGGAT
  361 ACTTAGAGTT CCTCATGTAG ACCTTGTGGC TCAGGGGCAG GTTGTTGCAA GATTGACCCC
  421 GGAAGTATAC TTTGGAATAT ACAAAGTACA ACCCAGTTTC ATTGATCACA AGGCCACCCT
  481 TCTTATACTT CACTCCAGAA AGCAGGACAA TTCCATAGGT GTCTTCCCAT TCCAGAGGCA
  541 TGGACCTTGA GTTGGACTTG CCTGTTAAAT GGGCCACTTT CCTCAGCTCC TTTTTTTCAG
  601 GGGGTGGACT GGGGTGGCCT ATTTGCTTCT CCAAAGATGA TGCTGTGTGC ATCTGGCTGG
  661 TAGACTCTCG GAGTTCTGCC AGCTCCTTCT GTAGGTGGAA GAGCTGAAAC ATCCCCAGGC
  721 CCAATCCTAC CAAGGCAACC AGAACCATGA AAAACATCAC AAGGAGACAC AGGCCTGTGC
  781 TGTGGTTCCC TCTCTTCTTC AGGGGTGGCA GCGGTAGTGG AGGCAGTGGT GGCGGCGGCG
841 GCGGAGGTGG TAGTGGTGGC GGTGGCGGTG GTGGTGGTGG CC
//
Primer: pAxcwit2-Fw
Sequence file: RDB15036_A7Egc.seq check
>02019_15036_A7Eg_1_pAxcwit2_Fw_D07_10_ABI24.ab1
    1 GCCCGCAGTG CCCTCCCCAC CACGTGGTCG GGGCCTCAGC TCCTCGGTCA CATCCAGCAT
   61 CACAGGCTGG TTCCTAATAT GTTTACCGCC ACACTCGCAG GGTCTGCACC TGGTGCGGGT
  121 CTCATCGTAC CTCAGCACCT TCCAGATCCA TCGTCGCGAG TCGACAATCG ATGTCGACAT
  181 TGATTATTGA CTAGTTATTA ATAGTAATCA ATTACGGGGT CATTAGTTCA TAGCCCATAT
  241 ATGGAGTTCC GCGTTACATA ACTTACGGTA AATGGCCCGC CTGGCTGACC GCCCAACGAC
  301 CCCCGCCCAT TGACGTCAAT AATGACGTAT GTTCCCATAG TAACGCCAAT AGGGACTTTC
  361 CATTGACGTC AATGGGTGGA GTATTTACGG TAAACTGCCC ACTTGGCAGT ACATCAAGTG
  421 TATCATATGC CAAGTACGCC CCCTATTGAC GTCAATGACG GTAAATGGCC CGCCTGGCAT
  481 TATGCCCAGT ACATGACCTT ATGGGACTTT CCTACTTGGC AGTACATCTA CGTATTAGTC
  541 ATCGCTATTA CCATGGTCGA GGTGAGCCCC ACGTTCTGCT TCACTCTCCC CATCTCCCCC
  601 CCCTCCCCAC CCCCAATTTT GTATTTATTT ATTTTTTAAT TATTTTGTGC AGCGATGGGG
661 GCGGGGGGGG GGGGGGGGGG
//
Primer: Amp_R
Sequence file: RDB15036_A7Egd.seq check
>02019_15036_A7Eg_1_Amp_R_E07_13_ABI24.ab1
    1 TAGGGGGAAC CAGGCGTGAG GAGGCTTGTA TCAGGGTTAT TGTCTCATGA GCGGATACAT
   61 ATTTGAATGT ATCCTATTAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA
  121 GTGCCACCTG ACGTCTAAGA AACCATTATT ATCATGACAT TAACCTATAA AAATAGGCGT
  181 ATCACGAGGC CCTTTCGTCT TCAAGAATTC CGGATCCGGG CCAATATGAT AATGAGGGGG
  241 TGGAGTTTGT GACGTGGCGC GGGGCGTGGG AACGGGGCGG GTGACGTAGT AGTGTGGCGG
  301 AAGTGTGATG TTGCAAGTGT GGCGGAACAC ATGTAAGCGA CGGATGTGGC AAAAGTGACG
  361 TTTTTGGTGT GCGCCGGTGT ACACAGGAAG TGACAATTTT CGCGCGGTTT TAGGCGGATG
  421 TTGTAGTAAA TTTGGGCGTA ACCGAGTAAG ATTTGGCCAT TTTCGCGGGA AAACTGAATA
  481 AGAGGAAGTG AAATCTGAAT AATTTTGTGT TACTCATAGC GCGTAATATT TGTCTAGGGC
  541 CGCGGGGACT TTGACCGTTT ACGTGGAGAC TCGCCCAGGT GTTTTTCTCA GGTGTTTTCC
  601 GCGTTCCGGG TCAAAGTTGG CGTTTTATTA TTATAGTCAG CATCGATGAG CTTGATAGCT
  661 TGGGCTGCAG GTCGAGGGAT CTCCATAAGA GAAGAGGGAC AGCTATGACT GGGAGTAGTC
  721 AGGAGAGGAG GAAAAATCTG GCTAGTAAAA CATGTAAGGA AAATTTTAGG GATGTTAAAG
  781 AAAAAAATAA CACAAAACAA AATATAAAAA AAATCTAACC TCAAGTCAAG GCTTTTCTAT
  841 GGAATAAGGA ATGGACAGCA GGGGGCTGTT TCATATACTG ATGACCTCTT TATAGCCAAC
  901 CCTTTGTTCA TGGCAGCCAG CATATGGGCA TATGTTGCCC AAACTCTAAA CCAAATACTC
  961 ATTCTGATGT TTTAATGATT TGCCCTCCCA TATGTCCTTC CGAGTGAGAG ACAACAAAAA
 1021 ATTCCCAACA CACTAATTGC ATGAAAATAA AATTTCCCTT TTATTAGGCC AGAAGTCAGA
 1081 TGGCTTCAAG GGGGCTTCAT GATGTCCCCA TATTTTTTGG GCCAGAAGGG AAAGGATCCT
1141 CAATTGTATT TGGTGGAGCA AGGGACAATT GGCCCACACA CGCAATCCAA CCATTGC
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Shinoura, N., Construction, propagation, and titer estimation of recombinant adenoviruses carrying proapoptotic genes. Human Gene Therapy, 9, 2683-2689 (1998). PMID 9874266. [link to RRC of NBRP]