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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

S-HA-pRc/CMV (#RDB02136)

Expressing HA-tagged fusion protein, CMV promoter.

Alternative name S-HA, single HA-tagged expression vector
Clone info. Expression vector. Expressing HA-tagged fusion protein under the control of CMV promoter.
Vector backbone pRc/CMV (Plasmid)
Size of vector backbone 5.5 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Growth conditions 37 C, LB+Amp
Depositor|Developer Takemoto, Yoshihiro | Hashimoto, Yasuhiro | Furuta, Masaaki | Sato, Mitsuru |
 
Sequence RDB02136z.seq
Additional map
Remarks, protocol and/or map (pdf) RDB02136.pdf

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (DNA Cell Biol., 16, 893-896 (1997)).
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (DNA Cell Biol., 16, 893-896 (1997))。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB02136 S-HA-pRc/CMV DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The S-HA-pRc/CMV was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB02136).

Reference section:

Takemoto, Y., Sato, M., Furuta, M., Hashimoto, Y., Expression plasmid vectors with convenient subcloning sites in lambda gt11 that efficiently produce detectable tagged proteins. DNA Cell Biol., 16, 893-896 (1997). PMID 9260932. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB13988_A6Cmp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016)
Region: CMV pro,T7 pro,HA,SP6 pro,bGH pA,M13 ori
Sequence file: RDB13988_A6Cma.seq check
>02136_13988_A6Cm_2_CMV-Forward_A01_01.ab1
    1 NNNNNNNNNN CNNTTGNNGC NNTGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC
   61 AGAGCTCTCT GGCTAACTAG AGAACCCACT GCTTACTGGC TTATCGAAAT TAATACGACT
  121 CACTATAGGG AGACCCAAGC TTCCATGGCC TACCCCTACG ACGTGCCCGA CTACGCCGCG
  181 GCCGCTCGAG CATGCATCTA GAGGGCCCTA TTCTATAGTG TCACCTAAAT GCTAGAGCTC
  241 GCTGATCAGC CTCGACTGTG CCTTCTAGTT GCCAGCCATC TGTTGTTTGC CCCTCCCCCG
  301 TGCCTTCCTT GACCCTGGAA GGTGCCACTC CCACTGTCCT TTCCTAATAA AATGAGGAAA
  361 TTGCATCGCA TTGTCTGAGT AGGTGTCATT CTATTCTGGG GGGTGGGGTG GGGCAGGACA
  421 GCAAGGGGGA GGATTGGGAA GACAATAGCA GGCATGCTGG GGATGCGGTG GGCTCTATGG
  481 CTTCTGAGGC GGAAAGAACC AGCTGGGGCT CGAGGGGGGA TCCCCACGCG CCCTGTAGCG
  541 GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG
  601 CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC
  661 CCCGTCAAGC TCTAAATCGG GNGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC
  721 TCGACCCCNA AAAACTTGAN TANGGTGANG GNTCACGTAG TGGGNCATCG CCCNGATAGA
  781 NGGNTTTTCG CCCTTTGACG TNNGANTNNN NNTTCTTNNN TANTGNNNTC NNGNNNNNAN
  841 NTNNNNCNNC ACTCNNCCCT ATNNNNGNNN ANTCNTTTGA NTNATNNNGN ANTTNNNNNN
901 NTNNNNNNNN NN
//
Primer: SV40pro_F_V2 (Pr0154)
Region: NeoR
Sequence file: RDB13988_A6Cmb.seq check
>02136_13988_A6Cm_2_SV40pro_F_V2_B01_04.ab1
    1 NNNNNNNNNN NNNNNNNNNN CNNNGCTTTT GCNNNNNNTC CCGGGNNCTT GGATATCCNT
   61 TTTCGGATCT GATCAAGAGA CAGGATGAGG ATCGTTTCGC ATGATTGAAC AAGATGGATT
  121 GCACGCAGGT TCTCCGGCCG CTTGGGTGGA GAGGCTATTC GGCTATGACT GGGCACAACA
  181 GACAATCGGC TGCTCTGATG CCGCCGTGTT CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT
  241 TTTTGTCAAG ACCGACCTGT CCGGTGCCCT GAATGAACTG CAGGACGAGG CAGCGCGGCT
  301 ATCGTGGCTG GCCACGACGG GCGTTCCTTG CGCAGCTGTG CTCGACGTTG TCACTGAAGC
  361 GGGAAGGGAC TGGCTGCTAT TGGGCGAAGT GCCGGGGCAG GATCTCCTGT CATCTCACCT
  421 TGCTCCTGCC GAGAAAGTAT CCATCATGGC TGATGCAATG CGGCGGCTGC ATACGCTTGA
  481 TCCGGCTACC TGCCCATTCG ACCACCAAGC GAAACATCGC ATCGAGCGAG CACGTACTCG
  541 GATGGAAGCC GGTCTTGTCG ATCAGGATGA TCTGGACGAA GAGCATCAGG GGCTCGCGCC
  601 AGCCGAACTG TTCGCCAGGC TCAAGGCGCG CATGCCCGAC GGCGAGGATC TCGTCGTGAC
  661 CCATGGCGAT GCCTGCTTGC CGAATATCAT GGTGGAAAAT GGCCGCTTTT CTGGATTCAT
  721 CGACTGTGGC CGGCTGGGTG TGGCGGACCG CTATCAGGAC ATAGCGTTGG CTACCCGTGA
  781 TATTGCTGAA GAGCTTGGCG GCGAATGGNC TGACCGCTTC CTCGTGCTTT ACGGTATCGC
  841 CGCTCCCGAT TCNCAGCGCA TCGCCTTCTA TCGCCTTCTT GACGANTNCT TCTGNNCGGG
  901 ANTCTGGGGG NTCGAAATGA CCGANNNAGC GACNCCCAAC CTGNCNTCAC NANANTTCGA
  961 TNNCNNCNGN CGCCNTCTAT GAAANNNNGG GCNNNNGNNN NNTTTTNNNG GNNNNNNNNN
1021 N
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Takemoto, Y., Expression plasmid vectors with convenient subcloning sites in lambda gt11 that efficiently produce detectable tagged proteins. DNA Cell Biol., 16, 893-896 (1997). PMID 9260932. [link to RRC of NBRP]