Resource data sheet
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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

S-T7-pRc/CMV (#RDB02137)

Expressing T7-tagged fusion protein, CMV promoter.

Alternative name S-T7, single T7-tagged expression vector
Clone info. Expression vector. Expressing T7-tagged fusion protein under the control of CMV promoter.
Vector backbone pRc/CMV (Plasmid)
Size of vector backbone 5.5 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Growth conditions 37 C, LB+Amp
Depositor|Developer Takemoto, Yoshihiro | Hashimoto, Yasuhiro | Furuta, Masaaki | Sato, Mitsuru |
 
Sequence RDB02137z.seq
Additional map
Remarks, protocol and/or map (pdf) RDB02137.pdf

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (DNA Cell Biol., 16, 893-896 (1997)).
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (DNA Cell Biol., 16, 893-896 (1997))。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB02137 S-T7-pRc/CMV DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The S-T7-pRc/CMV was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB02137).

Reference section:

Takemoto, Y., Sato, M., Furuta, M., Hashimoto, Y., Expression plasmid vectors with convenient subcloning sites in lambda gt11 that efficiently produce detectable tagged proteins. DNA Cell Biol., 16, 893-896 (1997). PMID 9260932. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB13052_A5A5.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward
Sequence file: RDB13052_A5A5a.seq check
>02137_13052_A5A5_1_CMV-Forward_H01_22.ab1
    1 CTTAACTCGC CCCATTGACG CAATGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG
   61 CAGAGCTCTC TGGCTAACTA GAGAACCCAC TGCTTACTGG CTTATCGAAA TTAATACGAC
  121 TCACTATAGG GAGACCCAAG CTTCCATGGC CAGCATGACC GGCGGCCAGC AGATGGGCGC
  181 GGCCGCTCGA GCATGCATCT AGAGGGCCCT ATTCTATAGT GTCACCTAAA TGCTAGAGCT
  241 CGCTGATCAG CCTCGACTGT GCCTTCTAGT TGCCAGCCAT CTGTTGTTTG CCCCTCCCCC
  301 GTGCCTTCCT TGACCCTGGA AGGTGCCACT CCCACTGTCC TTTCCTAATA AAATGAGGAA
  361 ATTGCATCGC ATTGTCTGAG TAGGTGTCAT TCTATTCTGG GGGGTGGGGT GGGGCAGGAC
  421 AGCAAGGGGG AGGATTGGGA AGACAATAGC AGGCATGCTG GGGATGCGGT GGGCTCTATG
  481 GCTTCTGAGG CGGAAAGAAC CAGCTGGGGC TCGAGGGGGG ATCCCCACGC GCCCTGTAGC
  541 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC
  601 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT
  661 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC
  721 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG
  781 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA
  841 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG
  901 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTTAAC
  961 AAAATATTAA CGCTTACAAT TTAAATATTT GCTTATACAA TCTTCCTGTT TTTGGGGCTT
 1021 TTCTGATTAT CAACCCGGGG GTGGTACCGA GCTCGAATTC TGTCAATGTG TGTTCAGTAG
 1081 GGTGTGAAAG TCCCCAGGCT CCCAGCAAGG CAGAGTATGC CAAAGCATGC ATCCTCAAAT
1141 TAGTTCAAGC CAACCCCAAA
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Takemoto, Y., Expression plasmid vectors with convenient subcloning sites in lambda gt11 that efficiently produce detectable tagged proteins. DNA Cell Biol., 16, 893-896 (1997). PMID 9260932. [link to RRC of NBRP]