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pAxCAhDCT (reverse) (#RDB02317)

Shuttle vector to produce rAd expressing human DCT

Alternative name 037C35, Dopachrome tautomerase
Clone info. Shuttle vector to produce recombinant adenovirus expressing Human DOPAchrome tautomerase/tyrosinase related protein 2 (TRP2) gene. Direction : reverse.
Comment Produce adenovirus by COS-TPC method.
Vector backbone pAxCAwt (RDB01678) (Cosmid, use packaging extracts for transforming E. coli host)
Size of vector backbone 44,820 bp
Selectable markers Amp^r
Growth conditions 37 C, LB+Amp
Gene/insert name Human DOPAchrome tautomerase (DT,DCT/TRP-2,TYRP2) cDNA
Depositor|Developer DNA Bank, |
 
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Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for not-for-profit research in the not-for-profit organization.
Additional terms and conditions:
Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
Remarks Remember that you will be working with samples containing infectious virus.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する。 本件リソースは非営利機関にのみ提供し、非営利研究にのみ利用することができる。
付加的使用条件：
CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Availability Shipping form Fee
RDB02317 pAxCAhDCT (reverse) Under QC test. Please contact us. DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The pAxCAhDCT (reverse) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB02317).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

reference Yokoyama, K., Cloning of the human DOPAchrome tautomerase/tyrosinase-related protein 2 gene and identification of two regulatory regions required for its pigment cell-specific expression. J. Biol. Chem., 269, 27080-27087(1994). PMID 7929451. [PubMed]
reference Yokoyama, K., Molecular cloning and functional analysis of a cDNA coding for human DOPAchrome tautomerase/tyrosinase-related protein-2. Biochim. Biophys. Acta, 1217, 317-321 (1994). PMID 8148378. [PubMed] [Article]