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pcDNA3-p73alpha

Expression vector for human p73 alpha, based on pcDNA3

Catalog number RDB02962
Resource name pcDNA3-p73alpha
Clone info. Expression vector for human p73 alpha, based on pcDNA3. cDNA fragment 106 to 2095nt of NM_005427.3 is cloned.
Comment Known differences (at least): C to T substitution at 629nt (Thr, synonym) of NM_005427.3; C to T substitution at 1922nt (Gly, synonym) of NM_005427.3.
Vector backbone pcDNA 3.1(+) (Plasmid)
Size of vector backbone 5,400 bp
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human p73 cDNA
Depositor|Developer Shimotohno, Kunitada |
Other clones in our bank
External Database
Human p73
          Reference sequence
            Y11416,NM_005427.3

          Distribution information

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          RDB02962 pcDNA3-p73alpha DNA solution Please contact us.

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Remarks, protocol and/or map (gif) RDB02962.gif

          Original reference

          original Ueda, Y., New p73 variants with altered C-terminal structures have varied transcriptional activities. Oncogene, 18, 4993-4998 (1999). PMID 10490834.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB15723_A8A4p1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV-Forward
          Sequence file: RDB15723_A8A4a.seq check
          >02962_15723_A8A4_CMV-Forward_C10_07_ABI24.ab1
              1 CTTTTCAAAT CCCGCCCATT GACGCAATGG GCGGTAGGCG TGTACGGTGG GAGGTCTATA
             61 TAAGCAGAGC TCTCTGGCTA ACTAGAGAAC CCACTGCTTA CTGGCTTATC GAAATTAATA
            121 CGACTCACTA TAGGGAGACC CAAGCTTGGT ACCGAGCTCG GATCCGGAAG ATGGCCCAGT
            181 CCACCGCCAC CTCCCCTGAT GGGGGCACCA CGTTTGAGCA CCTCTGGAGC TCTCTGGAAC
            241 CAGACAGCAC CTACTTCGAC CTTCCCCAGT CAAGCCGGGG GAATAATGAG GTGGTGGGCG
            301 GAACGGATTC CAGCATGGAC GTCTTCCACC TGGAGGGCAT GACTACATCT GTCATGGCCC
            361 AGTTCAATCT GCTGAGCAGC ACCATGGACC AGATGAGCAG CCGCGCGGCC TCGGCCAGCC
            421 CCTACACCCC AGAGCACGCC GCCAGCGTGC CCACCCACTC GCCCTACGCA CAACCCAGCT
            481 CCACCTTCGA CACCATGTCG CCGGCGCCTG TCATCCCCTC CAACACCGAC TACCCCGGAC
            541 CCCACCACTT TGAGGTCACT TTCCAGCAGT CCAGCACGGC CAAGTCAGCC ACCTGGACGT
            601 ACTCCCCGCT CTTGAAGAAA CTCTACTGCC AGATCGCCAA GACATGCCCC ATCCAGATCA
            661 AGGTGTCCAC CCCGCCACCC CCAGGCACTG CCATCCGGGC CATGCCTGTT TACAAGAAAG
            721 CGGAGCACGT GACCGACGTC GTGAAACGCT GCCCCAACCA CGAGCTCGGG AGGGACTTCA
            781 ACGAAGGACA GTCTGCTCCA GCCAGCCACC TCATCCGCGT GGAAGGCAAT AATCTCTCGC
            841 AGTATGTGGA TGACCCTGTC ACCGGCAGGC AGAGCGTCGT GGTGCCCTAT GAGCCACCAC
            901 AGGTGGGGGA CGGAATTCAC CACCATCCTG TACAACTTCA TGTGTAACAG CAGCTGTGTA
            961 GGGGGGCATG AACCGGGCGG GCCCATCCCT CATCATCATC ACCCTGGAGA TGCGGGATGG
           1021 GCAGGTGCTG GGCCGCCGTC CCTTTGAGGC CGCCATCTGC GCCTGTCCTG ACCGCGACGA
          1081 AAGCTGAATG AAGAACCACT ACCGGGGAGT AAGCAGGATC T
          //
          Primer: BGH_rev2
          Sequence file: RDB15723_A8A4b.seq check
          >02962_15723_A8A4_BGH_rev2_D10_10_ABI24.ab1
              1 CGTGCCAGGG TCAAGAGGCA CGGGGGAGGG GCAACAACAG ATGGCTGGCA ACTAGAAGGC
             61 ACAGTCGAGG CTGATCAGCG AGCTCTAGCA TTTAGGTGAC ACTATAGAAT AGGGCCCTCT
            121 AGATGCATGC TCGAGTCGAC TGGACACACA GGAAGGAGAG GGGAGGCAGC TTGGGTCTCT
            181 GGGCGGTGGC GCAGGCTGCA GCCAGGCGAG GCCCTCAGTG GATCTCGGCC TCCGTGAACT
            241 CCTCCTTGAT GGGCTGCTTG CGGGCCTTGC AGTCGGGCAG GTCGAAGCCG AAGTCCGCCC
            301 ACTCGTCAGG GCCACCGCCT GGGCCGCCGC GGTTGGGGAT GGTGATGGTG TGGCGCACGC
            361 GGAAGTGCAC GGCCTCCATG ACCCGCTGGC GCTGCAGTTC CCCTGAGCCG CCGATGGAGA
            421 TGGTGGCCGC GTTGCTAGAG CGGAGCAGCT GCTGCGCGGT GCTGTAGTCG TGGCCCTGCT
            481 TCAGGTCCTG CAGGCCCCGC CAGATGGTCA TGCGGTACTG CTCGGGGATC TTCAGGGCCC
            541 CCAGGTCCTC AATGGTCAGG TTCTGCAGGT GGTAAATGCT CTGTAACCCT TGGGAGGTGA
            601 AATACTCGAT GCAGTTTGGA CACCCCAATC CTGTTAAAAA ACTGACGAGG CTGGGGTCGG
            661 CGTGGTAGGG GGGTGGCGGA GTGCAGTGGG ACCCCGAGAC CATGGACTGG GCGCTGTGGC
            721 TGCTGCTCAT CTCGCCGTTG GCTGGCACTG CGTGGCCATG GTTGTTGAGC ATCCCGGGGC
            781 CCACGGGCCC CAGGTTGGGT GTAGCTGCCG AACTGTGCGG GGGAGGCTGG CCCACCAGCT
            841 GGTTGACGGA GGGCAGCTTG TTCATGCCCC CGTGCACCTT GTTCATGGGC GAGAGGACCG
            901 GCCCGTAGGA CGGGGGGCTG TAGGTGACTC GGCCTCTGTA GGAGCTGCTG CTGCTGCCGA
            961 TAGGAGTCCA CCAGTGGCTG CGGGCACCCA ACTCCATCAG CTCCAGGCTC TCTTTCAGCT
           1021 TTCATCAGGA TCTCAAAGTC TCCCGGCCTC GCCACCTGAA GGTAGTTACG TGTTCCTCGT
          1081 CTCCATGACG TCGGCATCTC ACATCGGGTC ACCAAGGACG GGGACGGCCA GGGGGGG
          //
          Primer: SV40pro_F
          Sequence file: RDB15723_A8A4c.seq check
          >02962_15723_A8A4_SV40pro_F_E10_13_ABI24.ab1
              1 GCTTGGGGCT TGGGACTTTC CAGAGTAGTG AGGAGGCTTT TTTGGAGGCC TAGGCTTTTG
             61 CAAAAAGCTC CCGGGAGCTT GTATATCCAT TTTCGGATCT GATCAAGAGA CAGGATGAGG
            121 ATCGTTTCGC ATGATTGAAC AAGATGGATT GCACGCAGGT TCTCCGGCCG CTTGGGTGGA
            181 GAGGCTATTC GGCTATGACT GGGCACAACA GACAATCGGC TGCTCTGATG CCGCCGTGTT
            241 CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG ACCGACCTGT CCGGTGCCCT
            301 GAATGAACTG CAGGACGAGG CAGCGCGGCT ATCGTGGCTG GCCACGACGG GCGTTCCTTG
            361 CGCAGCTGTG CTCGACGTTG TCACTGAAGC GGGAAGGGAC TGGCTGCTAT TGGGCGAAGT
            421 GCCGGGGCAG GATCTCCTGT CATCTCACCT TGCTCCTGCC GAGAAAGTAT CCATCATGGC
            481 TGATGCAATG CGGCGGCTGC ATACGCTTGA TCCGGCTACC TGCCCATTCG ACCACCAAGC
            541 GAAACATCGC ATCGAGCGAG CACGTACTCG GATGGAAGCC GGTCTTGTCG ATCAGGATGA
            601 TCTGGACGAA GAGCATCAGG GGCTCGCGCC AGCCGAACTG TTCGCCAGGC TCAAGGCGCG
            661 CATGCCCGAC GGCGAGGATC TCGTCGTGAC CCATGGCGAT GCCTGCTTGC CGAATATCAT
            721 GGTGGAAAAT GGCCGCTTTT CTGGATTCAT CGACTGTGGC CGGCTGGGTG TGGCGGACCG
            781 CTATCAGGAC ATAGCGTTGG CTACCCGTGA TATTGCTGAA GAGCTTGGCG GCGAATGGGC
            841 TGACCGCTTC CTCGTGCTTT ACGGTATCGC CGCTCCCGAT TCGCAGCGCA TCGCCTTCTA
            901 TCGCCTTCTT GACGAGTTCT TCTGAGCGGG ACTCTGGGGG TTCGAAATGA CCGACCAAGC
            961 GACGCCCAAC CCTGCCATCA CGAGATTTCG ATTCCCACCG CCCGCCCTTC TATGAAGGTT
           1021 GGGGCTTTCG GAATCGTTTT TCCAGGACGT CGGCTGGGAT GATTCCCTCC AGCGCGGGGA
           1081 ATCTCAATGC TTGGAGCTCT CGTCAACCTC GACTGGTTAT GCAGCTTTAT AATTGGTTAA
          1141 CA
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          original Ueda, Y., New p73 variants with altered C-terminal structures have varied transcriptional activities. Oncogene, 18, 4993-4998 (1999). PMID 10490834.

          2022.05.18

          GNP_filter3_RDBDEP_html_220516.pl