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pAxcwit (#RDB03120)

A dual cassette for constructing recombinant adenovirus

Clone info. A dual cassette for constructing recombinant adenovirus improved from pAxcw. Please note that pAxcwit has been improved as pAxcwit2 (RDB05212), which contains a Csp45I site and an additional PacI site for linearizing the cosmid vector by restriction enzymes.
Comment The pAxcwit cosmid is a derivative of pAxcw (RDB 917) and includes the Ad5 genome from 0 to 100 map units (mus). The cosmid vector, which is a promoter-less vector, can be used to clone an expression cassette that is driven by the promoter of interest at the unique SwaI site in the E1 region. The pAxcwit cosmid, digested with Csp45I, can generate rAd upon transfection of HEK293 cells. The Axcwit cosmid can also be used to generate rAd by the COS-TPC method [Miyake et al., Proc. Natl. Acad. Sci. USA 93, 1320-24, 1996].
Vector backbone charomid 9-11 (Cosmid, use packaging extracts for transforming E. coli host)
Size of vector backbone 11 kb
Selectable markers Amp^r
Gene/insert name AdV_5 E1, E3-deleted adenovirus 5 genome Genomic DNA
Depositor|Developer Saito, Izumu |
 
Sequence RDB03120y.seq
Sequence RDB03120z.seq
Additional map
Remarks, protocol and/or map (pdf) RDB03120.pdf

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Terashima, M., Exp. Med., 21, 931-936(2003)).
Remarks Remember that you will be working with samples containing infectious virus.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Terashima, M., Exp. Med., 21, 931-936(2003))。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB03120 pAxcwit Under QC test. Please contact us. DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The pAxcwit was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB03120).

Reference section:

Fukuda, H., Terashima, M., Koshikawa, M., Kanegae, Y., Saito, I., Possible mechanism of adenovirus generation from a cloned viral genome tagged with nucleotides at its ends. Microbiol. Immunol., 50, 643-654 (2006). PMID 16924150. [link to RRC of NBRP]
Terashima, M., Kondo, S., Kanegae, Y., Saito, I., [Japanese text] Exp. Med., 21, 931-936 (2003).

Further references such as user reports and related articles (go to bottom)


QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


References

Original, user report and related articles

original Fukuda, H., Possible mechanism of adenovirus generation from a cloned viral genome tagged with nucleotides at its ends. Microbiol. Immunol., 50, 643-654 (2006). PMID 16924150. [link to RRC of NBRP]
original Terashima, M., [Japanese text] Exp. Med., 21, 931-936 (2003).
user_report Kojima, Y., Oncolytic gene therapy combined with double suicide genes for human bile duct cancer in nude mouse models. J. Surg. Res. 157 (1): e63-70 (2009). PMID 19345377. [link to RRC of NBRP]